Whole Genome Sequencing of Danish Reveals a Genetically Diverse Collection with Clear Separation from .

() is a newly identified species that has been misidentified as () and is clinically relevant. We identified 25 genomes in our collection of whole genome sequenced . These genomes were compared to publicly available genomes and a phylogeny revealed seven clusters corresponding to seven clonal complexes.

Role of the Listeria monocytogenes 2-Cys peroxiredoxin homologue in protection against oxidative and nitrosative stress and in virulence.

Peroxiredoxins contribute to protection of some bacteria against reactive oxygen intermediates (ROIs) and reactive nitrogen intermediates (RNIs). Listeria monocytogenes, a facultative intracellular bacterial pathogen, interacts with ROIs and RNIs during infection. In this study, we investigated the involvement of the 2-Cys peroxiredoxin (Prx) homologue in L.

Acanthamoeba feature a unique backpacking strategy to trap and feed on Listeria monocytogenes and other motile bacteria.

Despite its prominent role as an intracellular human pathogen, Listeria monocytogenes normally features a saprophytic lifestyle, and shares many environmental habitats with predatory protozoa. Earlier studies claimed that Acanthamoeba may act as environmental reservoirs for L. monocytogenes, whereas others failed to confirm this hypothesis.

Axonal transport of Listeria monocytogenes and nerve-cell-induced bacterial killing.

Listeria monocytogenes (L. monocytogenes) can cause fatal brainstem encephalitis in both sheep and humans. Here we review evidence that the bacteria can be incorporated into axons following a primary cycle of replication in macrophages/dendritic cells after subcutaneous injection in projection areas of peripheral neurons.

Characterization of DegU, a response regulator in Listeria monocytogenes, involved in regulation of motility and contributes to virulence.

The degU (lmo2515) gene encodes a putative response regulator in the food-borne pathogen Listeria monocytogenes. It has 63% amino acid identity to the DegU response regulator of Bacillus subtilis. We have characterized the degU gene product in L.

Role of flagellin and the two-component CheA/CheY system of Listeria monocytogenes in host cell invasion and virulence.

The flagellum protein flagellin of Listeria monocytogenes is encoded by the flaA gene. Immediately downstream of flaA, two genes, cheY and cheA, encoding products with homology to chemotaxis proteins of other bacteria, are located. In this study we constructed deletion mutants with mutations in flaA.

CD44-regulated intracellular proliferation of Listeria monocytogenes.

CD44 has been implicated in immune and inflammatory processes. We have analyzed the role of CD44 in the outcome of Listeria monocytogenes infection in murine bone marrow-derived macrophages (BMM). Surprisingly, a dramatically decreased intracellular survival of L.

Colony-stimulating factor 1-dependent cells protect against systemic infection with Listeria monocytogenes but facilitate neuroinvasion.

By using mice genomically lacking the mononuclear phagocytic growth factor colony-stimulating factor 1 and thereby deficient in macrophage and dendritic cell populations, we show that these cells play a dual role: they constitute a major defense against systemic infection but also facilitate cerebral bacterial invasion by Listeria monocytogenes.