Publications by authors named "Lokendra R Singh"
This investigation aims to identify the reasons for the plumes' visibility, compare the stacks with other sinter plant stacks worldwide, and suggest countermeasures to completely stop the visibility of emissions. The appearance of the sinter plant stack emission changes with time and the background color of the sky due to the scattering effect of the sunlight and incidence angle. The flue gas samples were collected at the outlet of the emission control equipment and observed under optical and scanning electron microscopes.
View Article and Find Full Text PDFDiagnostic utility of LAMP PCR targeting bcsp-31 gene for human brucellosis infection.
Indian J Med Microbiol
September 2023
Article Synopsis
- - The study focuses on developing and evaluating a LAMP PCR test to diagnose human brucellosis, which is a significant public health issue, especially in low-resource settings where traditional PCR is costly and complex.
- - The researchers tested the LAMP PCR’s sensitivity and specificity using both cultured samples and clinical samples, finding it could detect brucella DNA quickly, with results showing 90.91% sensitivity and 99.37% specificity.
- - The LAMP PCR test demonstrated high accuracy in detecting brucellosis in patients, and it’s suggested to be a practical point-of-care option for diagnosing the disease in areas where conventional methods are not feasible.
Introduction: Brucellosis is a neglected zoonotic disease of major public health concern. In India, the incidence of brucellosis remains vastly underreported due to its non-specific clinical presentation and sub-optimal sensitivity of existing gold standard tests. Studies in Northeast India have shown high incidences of brucellosis in livestock, but the region lacks data on human brucellosis despite its high associated risk.
View Article and Find Full Text PDFIn the present study, a comprehensive proteomic analysis of Brucella melitensis (B. melitensis) strain ATCC23457 was carried out to investigate proteome alterations in response to in vitro-induced nutrient stress. Our analysis resulted in the identification of 2440 proteins, including 365 hypothetical proteins and 850 potentially secretory proteins representing ~77.
View Article and Find Full Text PDFArticle Synopsis
- The study developed a targeted mass spectrometry method to identify protein biomarkers for brucellosis in humans and livestock.
- It involved creating synthetic peptides from the Brucella proteome and optimizing assays using serum samples to detect specific peptides related to the disease.
- A key finding was that the peptide NAIYDVVTR was consistently detected in confirmed cases with 100% specificity, paving the way for more accurate brucellosis diagnostics.
Article Synopsis
- - The study investigates viral infections of the central nervous system (CNS) in Central India, focusing on identifying the causes using two methods of reverse transcriptase PCR (RT-PCR) in cerebrospinal fluid (CSF) and blood samples.
- - Testing 150 CSF and 50 blood samples, the research found that viral infections accounted for 14% of cases, with varicella zoster virus (VZV) and Japanese encephalitis virus (JEV) being the most common culprits.
- - Results indicate that the one-step RT-PCR method is more effective than the two-step RT-PCR method for detecting and quantifying viral RNA in clinical samples, highlighting its superior sensitivity.
Article Synopsis
- The text discusses species that cause brucellosis, a disease affecting both humans and animals.
- It presents the whole-genome sequence of strain CIIMS-BH-2, which is identified as belonging to biovar 2.
- The draft genome assembly of CIIMS-BH-2 measures 3.31 Mb and has a G+C content of 57.2%.
We report here the draft genome sequence of CIIMS-PH-1, an isolate obtained from a 16-day-old infant with septicemia. The draft genome of CIIMS-PH-1 consisted of 2,939,183 bp and is a member of sequence type 308, clonal complex 1, and lineage I.
View Article and Find Full Text PDFBackground & Objectives: Chikungunya virus (CHIKV) infection has recently witnessed re-emergence, affecting rural areas of India with high morbidity rates. This prospective study was conducted to evaluate seroprevalence and clinical manifestation in targeted villages reporting cases of CHIKV infection.
Methods: A total of 482 patients were recruited from Kalmana and Kothari villages of Ballarpur; Chandrapur district of Maharashtra state, India during CHIKV outbreaks in 2011-12.
Introduction: We evaluated the incidence and clinical outcome of patients with hypertensive acute ischemic stroke (AIS) admitted to a tertiary care center in Central India. In addition, we examined the status of stroke biomarkers namely neuron-specific enolase (NSE), glial specific protein (S-100ββ), and inter-α-trypsin inhibitor heavy chain 4(ITIH4) in the serum of patients suffering from AIS with hypertension (HTN) and without HTN.
Methods: A total of 104 patients with AIS were enrolled for the study.
Background: Demographic and clinical characteristics are known to influence the outcome in acute ischemic stroke (AIS) patients.
Purpose: This study is aimed at evaluating short- and long-term outcomes in diabetic AIS patients. In addition, the study also evaluates the impact of diabetes on the performance of indigenously reported biomarker, inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) and known biomarkers, neuron-specific enolase (NSE) and glial-derived S-100 beta beta protein (S-100ββ).