Multi-Omics of Growth in Chicken Exudate Reveals Molecular Remodelling Associated with Altered Virulence and Survival Phenotypes.

is the leading cause of foodborne human gastroenteritis in the developed world. Infections are largely acquired from poultry produced for human consumption and poor food handling is thus a major risk factor. Chicken exudate (CE) is a liquid produced from defrosted commercial chicken products that facilitates growth.

Global Acetylomics of Shows Lysine Acetylation Regulates CadF Adhesin Processing and Human Fibronectin Binding.

Lysine acetylation (KAc) is a reversible post-translational modification (PTM) that can alter protein structure and function; however, specific roles for KAc are largely undefined in bacteria. Acetyl-lysine immunoprecipitation and LC-MS/MS identified 5567 acetylated lysines on 1026 proteins from the gastrointestinal pathogen (∼63% of the predicted proteome). KAc was identified on proteins from all subcellular locations, including the outer membrane (OM) and extracellular proteins.

Mechanical Properties of Aramid/Carbon Hybrid Fiber-Reinforced Concrete.

In this study, aramid fiber (Kevlar 29 fiber) and carbon fiber were added into concrete in a hybrid manner to enhance the static and impact mechanical properties. The coupling agent presence on the surface of carbon fibers was spotted in Scanning Electron Microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) graphs. The carbon fiber with a coupling agent affected the mechanical strength of the reinforced concrete.

Integrated mass spectrometry-based multi-omics for elucidating mechanisms of bacterial virulence.

Despite being considered the simplest form of life, bacteria remain enigmatic, particularly in light of pathogenesis and evolving antimicrobial resistance. After three decades of genomics, we remain some way from understanding these organisms, and a substantial proportion of genes remain functionally unknown. Methodological advances, principally mass spectrometry (MS), are paving the way for parallel analysis of the proteome, metabolome and lipidome.

Proteomics of Growth in Deoxycholate Reveals Cj0025c as a Cystine Transport Protein Required for Wild-type Human Infection Phenotypes.

is a major cause of food-borne gastroenteritis. Proteomics by label-based two-dimensional liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identified proteins associated with growth in 0.1% sodium deoxycholate (DOC, a component of gut bile salts), and system-wide validation was performed by data-independent acquisition (DIA-SWATH-MS).

Proteomics Reveals Multiple Phenotypes Associated with -linked Glycosylation in .

is a major gastrointestinal pathogen generally acquired consumption of poorly prepared poultry. -linked protein glycosylation encoded by the gene cluster targets >80 membrane proteins and is required for both nonsymptomatic chicken colonization and full human virulence. Despite this, the biological functions of -glycosylation remain unknown.