Draft genome sequences of two multidrug-resistant strains isolated from wastewater in Spain.

We report the draft genome sequences of two strains isolated from wastewater in Galicia (NW Spain). Genomic analysis revealed that both strains harbor multiple genes associated with resistance to sulfonamides, fluoroquinolones, β-latamics, and other drugs.

Methodology to enable high-throughput imaging of Arabidopsis seedlings on cover glass-bottom multiwell plates.

High-throughput imaging enables rapid collection of large datasets and is used widely in many systems. However, this is not often used in plant-based systems due to issues related to the need to mount tissues and autofluorescence of plant metabolites. We therefore developed methodology enabling high-throughput imaging of Arabidopsis roots.

Occurrence and fate of CECs (OMPs, ARGs and pathogens) during decentralised treatment of black water and grey water.

Decentralised wastewater treatment is becoming a suitable strategy to reduce cost and environmental impact. In this research, the performance of two technologies treating black water (BW) and grey water (GW) fractions of urban sewage is carried out in a decentralised treatment of the wastewater produced in three office buildings. An Anaerobic Membrane Bioreactor (AnMBR) treating BW and a Hybrid preanoxic Membrane Bioreactor (H-MBR) containing small plastic carrier elements, treating GW were operated at pilot scale.

Detection of SARS-CoV-2 RNA in bivalve mollusks and marine sediments.

The presence of SARS-CoV-2 in wastewater pose the question of whether this new pandemic virus could be released into watercourses and potentially continue to finally reach coastal waters. In this study, we employed two bivalve molluscan species from the genus Ruditapes as sentinel organisms to investigate the presence of SARS-CoV-2 signals in the marine coastal environment. Estuarine sediments from the natural clam banks were also analyzed.

Clinic and electromyographic results of latissimus dorsi transfer for irreparable posterosuperior rotator cuff tears.

Background: This study examines the clinical and electromyographic results of latissimus dorsi transfer (LDT) using a combined open and arthroscopic technique for the treatment of symptomatic irreparable posterosuperior rotator cuff tears.

Methods: Between 2006 and 2009, LDT was performed in 14 patients (mean age 59 years) with massive and symptomatic irreparable posterosuperior rotator cuff tear. The patients were examined preoperatively and postoperatively with mean follow-up of 52 months using the Constant score, and the integrity of the latissimus dorsi (LD) transfer was assessed by ultrasound in all cases and by MRI in ten cases.

The shortage of critical care physicians: is there a solution?

Objective: The objective of this study is to provide a solution to the critical care physician shortage.

Data Sources: The data sources are Medline search of published articles regarding the critical care physician shortage, the current training model, and the roll of family physicians.

Data Extraction: The US population continues to age, increasing the need for critical care services due to the burden of acute and chronic illnesses.

Tracheal obstruction as a complication of tracheostomy tube malfunction: case report and review of the literature.

Tracheostomy is a procedure frequently used in the intensive care unit for prolonged ventilatory support, long-term airway maintenance, and to prevent the complications of long-term translaryngeal intubation. It is believed that it eases patient care and improves the process of weaning from mechanical ventilation. The timing of tracheostomy is controversial and most physicians prefer translaryngeal intubation for needs of up to 10 days and a tracheostomy if an artificial airway for more than 21 days is anticipated.

Bronchopleural fistulas: an overview of the problem with special focus on endoscopic management.

A bronchopleural fistula (BPF) is a communication between the pleural space and the bronchial tree. Although rare, BPFs represent a challenging management problem and are associated with high morbidity and mortality. By far, the postoperative complication of pulmonary resection is the most common cause, followed by lung necrosis complicating infection, persistent spontaneous pneumothorax, chemotherapy or radiotherapy (for lung cancer), and tuberculosis.

Lipopolysaccharide induces expression of fibronectin alpha 5 beta 1-integrin receptors in human monocytic cells in a protein kinase C-dependent fashion.

LPS is an outer-membrane glycolipid component of gram-negative bacteria known for its fervent ability to activate monocytic cells and for its potent proinflammatory capabilities. In addition, LPS triggers the release of cytokines and chemokines as well as cell-cell adhesion molecules. We postulate that LPS may also affect the expression of matrix-binding integrin receptors, thereby modulating cell-adhesive functions in monocytic cells.

Ethanol ingestion via glutathione depletion impairs alveolar epithelial barrier function in rats.

We determined that rats fed a liquid diet containing ethanol (36% of calories) for 6 wk had decreased (P < 0.05) net vectorial fluid transport and increased (P < 0.05) bidirectional protein permeability across the alveolar epithelium in vivo compared with rats fed a control diet.

Ethanol ingestion increases activation of matrix metalloproteinases in rat lungs during acute endotoxemia.

Previously we reported that alcohol abuse increases the incidence of the acute respiratory distress syndrome (ARDS) in septic patients, and that chronic ethanol ingestion in rats depletes alveolar epithelial glutathione and increases endotoxin-mediated lung edema. In this study we examined a potential mechanism by which ethanol-induced glutathione depletion could predispose to acute lung injury. We hypothesized that glutathione depletion activates matrix metalloproteinases (MMPs), thereby increasing degradation of the alveolar extracellular matrix (ECM) during sepsis.

Coexisting Sjögren's syndrome and sarcoidosis in the lung.

Context: Sjögren's syndrome (SS) and sarcoidosis are diseases of unknown origin that are considered to result from abnormal regulation of the immune system. Pulmonary involvement by SS and sarcoidosis may have similar clinical and radiographic manifestations, making it difficult for the clinician to distinguish between these diseases.

Objectives: This study was undertaken to analyze the characteristics of SS and sarcoidosis in the lung to identify distinguishing features that may assist clinicians in the differentiation of these conditions.