Publications by authors named "Lois E Murray"
Article Synopsis
- The diet of an animal significantly impacts the diversity and metabolic functions of its gut microbiome, particularly in species consuming lignified plant material like porcupines.
- Researchers analyzed the porcupine's microbiome and compared it to 20 other mammals, revealing insights into bacterial diversity across different mammalian groups.
- Results showed that while microbial composition varies, the functionalities are more consistent; particularly, porcupines and beavers possessed more cellulose-degrading enzyme genes compared to carnivores, emphasizing the link between diet and gut microbiome capabilities.
The Gram-negative intracellular pathogen Legionella pneumophila replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV), into which it abundantly releases its chaperonin, HtpB. To determine whether HtpB remains within the LCV or reaches the host cell cytoplasm, we infected U937 human macrophages and CHO cells with L. pneumophila expressing a translocation reporter consisting of the Bordetella pertussisa denylate cyclase fused to HtpB.
View Article and Find Full Text PDFGcs1 is an Arf GTPase-activating protein (Arf-GAP) that mediates Golgi-ER and post-Golgi vesicle transport in yeast. Here we show that the Snc1,2 v-SNAREs, which mediate endocytosis and exocytosis, interact physically and genetically with Gcs1. Moreover, Gcs1 and the Snc v-SNAREs colocalize to subcellular structures that correspond to the trans-Golgi and endosomal compartments.
View Article and Find Full Text PDF