Publications by authors named "Lloyd D Murphy"
Article Synopsis
- - Mammalian cells communicate through surface interactions, and proteoglycans play a key role by carrying large sugar chains that help recruit signaling molecules, yet only a few proteoglycans are currently identified.
- - Two enzymes, XT1 and XT2, are responsible for the initial step of adding sugars to proteins but their redundancy complicates the study of proteoglycans.
- - The research introduces a method called bump-and-hole engineering to modify these enzymes, allowing for the specific addition of a chemically marked sugar to proteins, enabling visualization and detailed analysis of proteoglycan structures in cells.
Robust methods for the synthesis of mixed phosphotriesters are essential to accelerate the development of novel phosphate-containing bioactive molecules. To enable efficient cellular uptake, phosphate groups are commonly masked with biolabile protecting groups, such as -acyl-2-thioethyl (SATE) esters, that are removed once the molecule is inside the cell. Typically, bis-SATE-protected phosphates are synthesised through phosphoramidite chemistry.
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