Antimicrobial Effects of Aqueous Extract from Calyces of in CD-1 Mice Infected with Multidrug-Resistant Enterohemorrhagic and S Typhimurium.

To determinate the antimicrobial effect of chloramphenicol and aqueous extract against multidrug-resistant enterohemorrhagic (EHEC) and serovar Typhimurium in CD-1 mice. Aqueous extract was isolated from calyces. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of chloramphenicol and aqueous extract were determined for EHEC and .

Hibiscus Acid and Chromatographic Fractions from Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria.

The anti-microbial properties of acetone extracts from calyces, fractions isolated by silica gel chromatography and hibiscus acid purified from some of these fractions and additionally identified by nuclear magnetic resonance spectroscopy, mid-infrared spectroscopy and X-ray diffraction, were studied against both multidrug-resistant strains and pathogenic bacteria. Gel diffusion was used to determine the anti-microbial effects. The mode of action of hibiscus acid was determined by crystal violet assay.