A turn-on fluorescent nanosensor for HS detection and imaging in inflammatory cells and mice.

Hydrogen sulfide (HS) is an endogenously generated gaseous signaling molecule and is known to be involved in the occurrence and development of inflammation. To better understand its physiological and pathological process of inflammation, reliable tools for HS detection in living inflammatory models are desired. Although a number of fluorescent sensors have been reported for HS detection and imaging, water-soluble and biocompatibility nanosensors are more useful for imaging in vivo.

Construction of a robust turn-on fluorescence NIR sensor for rapid detection and imaging of ONOO in inflammatory models.

Peroxynitrite (OONO) is closely related to the occurrence and development of health and inflammatory diseases. The physiological and pathological results of OONO are related to the local concentration of ONOO. Therefore, to develop of a simple, rapid and reliable OONO detection tool is badly needed.

Diagnosis of prenatal 22q11.2 duplication syndrome: a two-case study.

The objective of the study was to perform the prenatal diagnosis of two foetuses with 22q11.2 duplication for 2.5 Mb after noninvasive prenatal testing (NIPT), and to explore the prenatal diagnosis and genetic characteristics of these foetuses.

Markers Associated With Tumor Recurrence in Patients With Breast Cancer Achieving a Pathologic Complete Response After Neoadjuvant Chemotherapy.

Background: Patients who achieve a tumor pathologic complete response (pCR) after neoadjuvant chemotherapy (NAC) have better outcomes than patients with residual tumor. However, tumors still recur in the pCR patients. Therefore, we aim to explore factors associated with tumor recurrence in this patient population.

Clinical and antibody characteristics reveal diverse signatures of severe and non-severe SARS-CoV-2 patients.

Background: COVID-19 pandemic continues, clarifying signatures in clinical characters and antibody responses between severe and non-severe COVID-19 cases would benefit the prognosis and treatment.

Methods: In this study, 119 serum samples from 37 severe or non-severe COVID-19 patients from the First People's Hospital of Yueyang were collected between January 25 and February 18 2020. The clinical features, antibody responses targeting SARS-CoV-2 spike protein (S) and its different domains, SARS-CoV-2-specific Ig isotypes, IgG subclasses, ACE2 competitive antibodies, binding titers with FcγIIa and FcγIIb receptors, and 14 cytokines were comprehensively investigated.

Systemic perturbations of key metabolites in diabetic rats during the evolution of diabetes studied by urine metabonomics.

Background: Elucidation of metabolic profiles during diabetes progression helps understand the pathogenesis of diabetes mellitus. In this study, urine metabonomics was used to identify time-related metabolic changes that occur during the development of diabetes mellitus and characterize the biochemical process of diabetes on a systemic, metabolic level.

Methodology/principal Findings: Urine samples were collected from diabetic rats and age-matched controls at different time points: 1, 5, 10, and 15 weeks after diabetes modeling.

Acceleration of diabetic-wound healing with PEGylated rhaFGF in healing-impaired streptozocin diabetic rats.

Molecular modification with polyethylene glycol (PEGylation) is an effective approach to improve protein biostability, in vivo lifetime and therapeutic potency. In the present study, the recombinant human acid fibroblast growth factor (rhaFGF) was site-selectively PEGylated with 20 kDa mPEG-butyraldehyde. Mono-PEGylated rhaFGF was purified to near homogeneity by Sephadex G 25-gel filtration followed by a Heparin Sepharose TM CL-6B affinity chromatography.

Improved refractory wound healing with administration of acidic fibroblast growth factor in diabetic rats.

The aim of the present study is to investigate the effect and mechanism of acidic fibroblast growth factor (aFGF) on treating refractory wound of diabetic rats. SD rats were randomly divided into control group, diabetes group, and aFGF group. Ulcer skin tissues of three groups of rats were respectively collected on days 7 and 14 after establishment of ulcer model for biochemical test, pathological section and immunohistochemistry to comprehensively evaluate the treatment effect of aFGF on diabetic ulcer.

1H NMR-based metabonomic analysis of metabolic changes in streptozotocin-induced diabetic rats.

Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia, hypoinsulinemia, and ketosis. To access the biochemical process of diabetes, we applied quantitative (1)H NMR-based metabonomics to analyze urine, serum, and liver extracts from streptozotocin-induced diabetic rats. Principle component analysis (PCA) of (1)H NMR spectra disclosed metabolic pattern differences between diabetic and control rats, and identified the related metabolic changes.

Equilibrium solubility and dissolution rate of the lead phosphate chloropyromorphite.

Phosphate addition to lead-contaminated soils can immobilize lead in situ through the formation of lead phosphate minerals such as chloropyromorphite (Pb5(PO4)3Cl). The long-term stability of lead immobilized in lead phosphate precipitates depends on the equilibrium solubility and dissolution rates of the lead phosphate solids. The equilibrium solubility and dissolution kinetics of chloropyromorphite were quantified in a series of batch and flow-through reactors.