Effects of capsulorhexis size and position on post-surgical IOL alignment.

In cataract surgery, post-surgical stability of the intraocular lens plays a major role. This study aims to explore how the size and decentration of the capsulorhexis affect intraocular lens decentration and tilt by using numerical methods. Finite element models included zonules, ciliary body, capsular bag, and an IOL with two open-loop haptics were built.

Comparative genomics and phylogenetic analysis of six Malvaceae species based on chloroplast genomes.

The Malvaceae family, comprising 9 subfamilies and 4,225 species, includes economically significant taxa, such as Ceiba pentandra, Gossypium ekmanianum, Gossypium stephensii, Kokia drynarioides, Talipariti hamabo, and Durio zibethinus. Chloroplast (cp) genome research is crucial for elucidating the evolutionary divergence and species identification within this family. In this study, we assembled and annotated cp genomes of six Malvaceae species, conducting comprehensive comparative genomic and phylogenomic analyses.

The Effect of the Zonular Fiber Angle of Insertion on Accommodation.

With age, there is an anterior shift of the ciliary body in the eye, which alters the angle of zonular insertion in older eyes compared with younger eyes. This study aims to simulate lens accommodation with different zonular angles to consider the influence of zonular position on lens accommodative capacity. Models were constructed based on lenses aged 11, 29, and 45 years using a 2D axisymmetric structure that included a capsule, cortex, nucleus, and zonular fibers.

Chromosome-scale genome assembly of oil-tea tree Camellia crapnelliana.

Camellia crapnelliana Tutch., belonging to the Theaceae family, is an excellent landscape tree species with high ornamental values. It is particularly an important woody oil-bearing plant species with high ecological, economic, and medicinal values.

FARPA-based tube array coupled with quick DNA extraction enables ultra-fast bedside detection of antibiotic-resistant pathogens.

Rapid and accurate detection of pathogens and antimicrobial-resistant (AMR) genes of the pathogens are crucial for the clinical diagnosis and effective treatment of infectious diseases. However, the time-consuming steps of conventional culture-based methods inhibit the precise and early application of anti-infection therapy. For the prompt treatment of pathogen-infected patients, we have proposed a novel tube array strategy based on our previously reported FARPA (FEN1-aided recombinase polymerase amplification) principle for the ultra-fast detection of antibiotic-resistant pathogens on site.

Novel PIKfyve/Tubulin Dual-target Inhibitor as a Promising Therapeutic Strategy for B-cell Acute Lymphoblastic Leukemia.

Objective: In B-cell acute lymphoblastic leukemia (B-ALL), current intensive chemotherapies for adult patients fail to achieve durable responses in more than 50% of cases, underscoring the urgent need for new therapeutic regimens for this patient population. The present study aimed to determine whether HZX-02-059, a novel dual-target inhibitor targeting both phosphatidylinositol-3-phosphate 5-kinase (PIKfyve) and tubulin, is lethal to B-ALL cells and is a potential therapeutic for B-ALL patients.

Methods: Cell proliferation, vacuolization, apoptosis, cell cycle, and in-vivo tumor growth were evaluated.

Evaluation the Effect of Sonodynamic Therapy with 5-Aminolevulinic Acid and Sodium Fluorescein by Preclinical Animal Study.

Sonodynamic therapy (SDT) is a novel tumor treatment that combines biosafe sonosensitizers and noninvasive focused ultrasound to eradicate solid tumors. Sonosensitizers such as 5-aminolevulinic acid and fluorescein have great potential in tumor treatment. Here, rodent subcutaneous and brain tumor models were used to evaluate the treatment effect of both 5-ALA- and fluorescein-mediated SDT.

Comparative Genomic Analysis of Asian Cultivated Rice and Its Wild Progenitor () Has Revealed Evolutionary Innovation of the Pentatricopeptide Repeat Gene Family through Gene Duplication.

The pentatricopeptide repeat () gene family is one of the largest gene families in land plants. However, current knowledge about the evolution of the gene family remains largely limited. In this study, we performed a comparative genomic analysis of the gene family in and its wild progenitor, , and outlined a comprehensive landscape of gene duplications.

Ammonia borane positively regulates cold tolerance in Brassica napus via hydrogen sulfide signaling.

Background: Cold stress adversely influences rapeseeds (Brassica napus L.) growth and yield during winter and spring seasons. Hydrogen (H) is a potential gasotransmitter that is used to enhance tolerance against abiotic stress, including cold stress.

Preclinical Evaluation of the Multiple Tyrosine Kinases Inhibitor Anlotinib in Leukemia Stem Cells.

Leukemia stem cells (LSCs) constitute the critical barrier to the cure of acute myeloid leukemia (AML) due to their chemoresistance and immune evasion property. Herein, the role of anlotinib, a multiple tyrosine kinase inhibitor, in killing LSCs and regulating chemoresistance and immune evasion was explored. Anlotinib treatment induced apoptosis of LSC-like cells as well as primary AML LSCs, while sparing the normal mononuclear cells in vitro.

Multiplexed and Rapid AST for Infection by Simultaneously Pyrosequencing Multiple Barcodes Each Specific to an Antibiotic Exposed to a Sample.

Antimicrobial susceptibility testing (AST) is an effective way to guide antibiotic selection. However, conventional culture-based phenotypic AST is time-consuming. The key point to shorten the test is to quantify the small change in the bacterial number after the antibiotic exposure.

High-Sensitivity and Environmentally Friendly Humidity Sensors Deposited with Recyclable Green Microspheres for Wireless Monitoring.

The reliable, high-sensitive, wireless, and affordable requirements for humidity sensors are needed in high-precision measurement fields. Quartz crystal microbalance (QCM) based on the piezoelectric effect can accurately detect the mass changes at the nanogram level. However, water-capture materials deposited on the surface of QCM generally show disadvantages in either cost, sensitivity, or recyclability.

Pharmacological targeting PIKfyve and tubulin as an effective treatment strategy for double-hit lymphoma.

Double-hit lymphoma is one of the most aggressive and refractory lymphoma subtypes with recurrent genetic abnormalities of MYC and BCL-2 or BCL6 rearrangement, leading to a poor prognosis in the present clinical practice. Therefore, new therapeutic strategies for eliminating double-hit lymphomas are urgently needed. Here, we reported that HZX-02-059, a novel PIKfyve and tubulin dual-target inhibitor, showed a highly cytotoxic activity against double-hit lymphoma cell lines in vitro and in vivo through a noncanonical caspase-independent cell death, methuosis.

The complete chloroplast genome sequence of Vahl. (Poaceae).

Vahl. belongs to the Pooideae subfamily of Poaceae. In this study, we sequenced and assembled the complete chloroplast genome of The complete chloroplast genome was 134,718 bp in size, including a large single-copy region of 80,540 bp, a small single-copy region of 11,806 bp and a pair of reverse repeats of 21,186 bp in size.

Characterization of the chloroplast genome sequence of (L.C.Chia, H.L.Fung & Y.L.Yang) N.H.Xia (Poaceae).

(L.C.Chia, H.

The complete chloroplast genome sequence of Hack. (Poaceae).

Hack. belongs to the Arundionideae subfamily of Poaceae. In this study, we sequenced and assembled the complete chloroplast genome of The complete chloroplast genome was 136,919 bp in size, including a large single copy region of 82,039 bp, a small single-copy region of 12,108 bp and a pair of reverse repeats of 21,386 bp in size.

Characterization of chloroplast genome of , a highly adaptable cereal crop with high nutritional reputation.

(L.) Gaertn. is a kind of highly adaptable cereal crop with a high nutritional value with the reputation of 'black pearl'.

Circulating tumour cells at baseline and late phase of treatment provide prognostic value in breast cancer.

To determine the prognostic value of the timing of circulating breast tumour cell measurement during treatment, peripheral blood from 164 patients with breast disease was collected. Circulating tumour cells (CTCs) were enriched by using immunomagnetic nanospheres (IMNs) and were identified by using immunofluorescent staining. The CTC shows nuclear-positive, EpCAM-positive, CK19-positive, and CD45-negative.

Gapless indica rice genome reveals synergistic contributions of active transposable elements and segmental duplications to rice genome evolution.

The ultimate goal of genome assembly is a high-accuracy gapless genome. Here, we report a new assembly pipeline that is used to produce a gapless genome for the indica rice cultivar Minghui 63. The resulting 397.

Sensitive Formaldehyde Detection with QCM Sensor Based on PAAm/MWCNTs and PVAm/MWCNTs.

Two formaldehyde detection methods are proposed by applying composite film quartz crystal microbalance (QCM) sensors. QCM sensor coated with PAAm/MWCNTs and PVAm/MWCNTs shows excellent characteristics of lower limit and high sensitivity. The lower limit of PVAm/MWCNTs is 0.

Molecular Hydrogen Maintains the Storage Quality of Chinese Chive through Improving Antioxidant Capacity.

Chinese chive usually becomes decayed after a short storage time, which was closely observed with the redox imbalance. To cope with this practical problem, in this report, molecular hydrogen (H) was used to evaluate its influence in maintaining storage quality of Chinese chive, and the changes in antioxidant capacity were also analyzed. Chives were treated with 1%, 2%, or 3% H, and with air as the control, and then were stored at 4 ± 1 °C.

Disulfiram/copper shows potent cytotoxic effects on myelodysplastic syndromes via inducing Bip-mediated apoptosis and suppressing autophagy.

Patients with myelodysplastic syndromes (MDS) who resist or fail to respond to hypomethylating agents (HMAs) show very poor outcomes and have no effective treatment strategies. Therefore, new therapeutic approaches are urgently needed for MDS patients harboring adverse prognostic factors. Repurposing disulfiram (DSF), an alcohol-abuse drug, with or without Copper (Cu) has attracted considerable attentions as a candidate anti-tumor therapy in diverse malignancies.