Exporter engineering is a promising strategy to construct high-yield Streptomyces for natural product pharmaceuticals in industrial biotechnology. However, available exporters are scarce, due to the limited knowledge of bacterial transporters. Here, we built a workflow for exporter mining and devised a tunable plug-and-play exporter (TuPPE) module to improve the production of macrolide biopesticides in Streptomyces.
View Article and Find Full Text PDFSynth Syst Biotechnol
September 2020
Dramatic decrease of sugar uptake is a general phenomenon in at stationary phase, when antibiotics are extensively produced. Milbemycins produced by are a group of valuable macrolide biopesticides, while the low yield and titer impede their broad applications in agricultural field. Considering that inadequate sugar uptake generally hinders titer improvement of desired products, we mined the underlying sugar uptake systems and fine-tuned their expression in this work.
View Article and Find Full Text PDFIn this paper, a grating-coupled surface plasmon resonance concentration sensor employing a gold and indium tin oxide (Au/ITO) nanoparticle composite instead of metal is proposed. The structure and material parameters of the sensor are discussed and analyzed. Taking the ethylene glycol concentration as an example, the influence of the nanocomposite on the wave vector matching, the influence of the refractive index of the medium to be tested and the influence of the concentration on the refractive index were analyzed in detail.
View Article and Find Full Text PDFA novel actinobacterium, designated strain NEAU-FJL2(T), was isolated from a tomato root (Solanum lycopersicum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-FJL2(T) are consistent with the description of the genus Dactylosporangium.
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
August 2015
A novel endophytic actinomycete, strain NEAU-TX1-15(T), was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15(T). Morphological and chemotaxonomic properties of strain NEAU-TX1-15(T) are consistent with the description of the genus Actinoallomurus.
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