Functional analysis of the key BrSWEET genes for sugar transport involved in the Brassica rapa-Plasmodiophora brassicae interaction.

Plasmodiophora brassicae, the causative agent of clubroot disease, establishes a long-lasting parasitic relationship with its host by inducing the expression of sugar transporters. Previous studies have indicated that most BrSWEET genes in Chinese cabbage are up-regulated upon infection with P. brassicae.

Genome-Wide Identification and Characterization of the Trehalose-6-Phosphate Synthetase Gene Family in Chinese Cabbage () and during Their Interaction.

Trehalose is a nonreducing disaccharide that is widely distributed in various organisms. Trehalose-6-phosphate synthase (TPS) is a critical enzyme responsible for the biosynthesis of trehalose, which serves important functions in growth and development, defense, and stress resistance. Although previous studies have found that the clubroot pathogen Plasmodiophora brassicae can lead to the accumulation of trehalose in infected Arabidopsis organs, it has been proposed that much of the accumulated trehalose is derived from the pathogen.

Sugar Transporters in : Genome-Wide Identification and Functional Verification.

, an obligate intracellular pathogen, can hijack the host's carbohydrates for survival. When the host plant is infected by , a large amount of soluble sugar accumulates in the roots, especially glucose, which probably facilitates the development of this pathogen. Although a complete glycolytic and tricarboxylic acid cycle (TCA) cycle existed in , very little information about the hexose transport system has been reported.

Sensitive immunoassay of von Willebrand factor based on fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticles.

Graphene quantum dots (GQDs) and core-shell Ag@Au nanoparticles (Ag@Au NPs) were synthetized and they were characterized by transmission electron microscope and X-ray photoelectron spectra, respectively. Von Willebrand factor antibody (vWF Ab) was bound on Ag@Au NPs to construct Ag@Au-Ab nanocomposites (Ag@Au-Ab NCs). The fluorescence of GQDs could be effectively quenched by the prepared nanocomposites owing to fluorescence resonance energy transfer (FRET).

One-pot preparation of conductive composite containing boronic acid derivative for non-enzymatic glucose detection.

The composite consisting of poly(azure A), gold nanoparticles and 4-mercaptophenylboronic acid (PAA-AuNPs-MPBA) was prepared on the glassy carbon electrode surface by using a one-pot electropolymerization protocol. The generation of poly(azure A) film, the reduction of HAuCl and the binding of MPBA on metallic gold were simultaneously achieved in the cyclic voltammetric scan process, which was verified by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy measurements. MPBA on the composite could capture glucose based on the specific boronic acid-diol binding and form a stable 5-membered cyclic boronate ester, which prevented the penetration and the charge transfer of the ferri-/ferrocyanide couple on the electrode surface.

Highly sensitive turn-on fluorescence detection of thrombomodulin based on fluorescence resonance energy transfer.

As an integral glycoprotein on the surface of endothelial cells, thrombomodulin (TM) has very high affinity for thrombin. TM has been regarded to be a marker of endothelial damage since it can be released during endothelial cell injury. In this work, a highly sensitive fluorescence method for the quantitative detection of TM was developed.

Real-time monitoring of oxidative injury of vascular endothelial cells and protective effect of quercetin using quartz crystal microbalance.

The adhesion, spreading, and proliferation of human umbilical vein endothelial cell line (HUVEC-C) cells, on a gold electrode were monitored using quartz crystal microbalance (QCM) measurements. The viscodensity effect caused by the normal action of the cells led to a decrease of the resonant frequency and increase of the motional resistance. The oxidative injury of HUVEC-C cells appeared immediately with the addition of HO, exhibiting the decline of cellular spreading area and cell coverage on the electrode surface and resulting in inverted QCM responses.

Non-enzymatic detection of glucose using poly(azure A)-nickel modified glassy carbon electrode.

A simple, sensitive and selective non-enzymatic glucose sensor was constructed in this paper. The poly(azure A)-nickel modified glassy carbon electrode was successfully fabricated by the electropolymerization of azure A and the adsorption of Ni(2+). The Ni modified electrode, which was characterized by scanning electron microscope, cyclic voltammetry, electrochemical impedance spectra and X-ray photoelectron spectroscopy measurements, respectively, displayed well-defined current responses of the Ni(III)/Ni(II) couple and showed a good activity for electrocatalytic oxidation of glucose in alkaline medium.

Label-Free and Sensitive Detection of Thrombomodulin, a Marker of Endothelial Cell Injury, Using Quartz Crystal Microbalance.

Thrombomodulin (TM), an integral glycoprotein on the surface of endothelial cells, can be released during endothelial cell injury and the levels of serum TM are regarded as an important parameter of activity in vasculitides in vivo. Quantitative detection of TM and investigation on the release of soluble thrombomodulin (sTM) by the injured HUVEC-C cells using quartz crystal microbalance (QCM) were achieved in this work. Anti-antibody (AAb) and bovine serum albumin (BSA) were bound on gold nanoparticles (GNPs) to construct BSA-GNPs-AAb nanocomposites and they were characterized by transmission electron microscope, UV-vis, and infrared spectrophotometry, respectively.