The complete mitochondrial genome of the Alpine black swallowtail, Papilio Maackii (Insecta: Lepidoptera: Papilionidae).

The complete mitochondrial genome (mitogenome) of the swallowtail butterfly Papilio maackii has been completed. It is 15,357 bp in length, and contains the typical complement of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA), and 2 ribosomal RNA (rRNA) genes. Two A + T-rich regions are included in this mitogenome.

Wideband tunable optoelectronic oscillator based on a phase modulator and a tunable optical filter.

A widely tunable optoelectronic oscillator (OEO) based on a broadband phase modulator and a tunable optical bandpass filter is proposed and experimentally demonstrated. A tunable range from 4.74 to 38.

The complete mitochondrial genome of the Chinese peacock, Papilio bianor (Insecta: Lepidoptera: Papilionidae).

The sequence of the mitochondrial genome (mitogenome) of the Chinese peacock Papilio bianor has been completed. It is 15,332 bp with an A + T content of 80.6% and contains the typical complement of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA), and 2 ribosomal RNA (rRNA) genes.

Systematic analysis of the gene expression in the livers of nonalcoholic steatohepatitis: implications on potential biomarkers and molecular pathological mechanism.

Non-alcoholic steatohepatitis (NASH) is a severe form of non-alcoholic fatty liver disease (NAFLD). The molecular pathological mechanism of NASH is poorly understood. Recently, high throughput data such as microarray data together with bioinformatics methods have become a powerful way to identify biomarkers and to investigate pathogenesis of diseases.

[Expression and application research of ochratoxin A mimotope by filamentous phage pVIII display system].

Objective: Traditional ochratoxin A(OTA) competitive antigen are high toxicity, high price and difficult preparation. Non-toxic and easy prepared OTA competitive antigen substitutes were expressed by recombinant filamentous phage which have OTA mimotope displayed on its p VI surface.

Methods: Recombinant phagemid pC89-COTA which contain OTA mimotope nucleotide sequence was constructed.

Repair of rabbit femoral condyle bone defects with injectable nanohydroxyapatite/chitosan composites.

Repair of massive bone loss remains a challenge to the orthopaedic surgeons. Autologous and allogenic bone grafts are choice for bone reconstructive surgery, but limited availability, risks of transmittable diseases and inconsistent clinical performances have prompted the development of tissue engineering. In the present work, the bone regeneration potential of nanohydroxyapatite/chitosan composite scaffolds were compared with pure chitosan scaffolds when implanted into segmental bone defects in rabbits.

Effect of dietary advanced glycation end products on mouse liver.

Unlabelled: The exact pathophysiology of non-alcoholic steatohepatitis (NASH) is not known. Previous studies suggest that dietary advanced glycation end products (AGEs) can cause oxidative stress in liver. We aim to study the effects of dietary AGEs on liver health and their possible role in the pathogenesis of NASH.

High performance light-colored nitrile-butadiene rubber nanocomposites.

High mechanical performance nitrile-butadiene rubber (NBR) with light color was fabricated by the method of in situ formation of zinc disorbate (ZDS) or magnesium disorbate (MDS). The in situ formed ZDS and its polymerization via internal mixing was confirmed by X-ray diffaraction. The mechanical properties, ageing resistance, morphology and the dynamic mechanical analysis were fully studied.

[Percutaneous radiofrequency ablation versus hepatic resection for small hepatocellular carcinoma: a meta analysis].

Objective: To evaluate the curative effect of percutaneous radiofrequency ablation (RFA) and hepatic resection (RES) for small hepatocarcinoma eligible for Milan criterion using meta analysis method.

Methods: Retrieved clinical trials comparing percutaneous radiofrequency ablation with RES for small hepatocarcinoma published from 1990 to 2010. A meta-analysis was conducted to estimate overall survival and disease free survival.

Experimental demonstration of 1.08 Tb/s PDM CO-SCFDM transmission over 3170 km SSMF.

Coherent optical single-carrier frequency-division-multiplexing (CO-SCFDM) is a promising candidate for future high-speed long-haul optical fiber transmission system. Being a modified form of coherent optical orthogonal frequency division multiplexing (CO-OFDM), the CO-SCFDM can inherit the advantages such as low computation complexity and high flexibility, while suffers less nonlinear impairment due to much lower peak-to-average power ratio (PAPR). In this paper, we experimentally demonstrate 1.

Mitogenomic analysis of the genus Panthera.

The complete sequences of the mitochondrial DNA genomes of Panthera tigris, Panthera pardus, and Panthera uncia were determined using the polymerase chain reaction method. The lengths of the complete mitochondrial DNA sequences of the three species were 16990, 16964, and 16773 bp, respectively. Each of the three mitochondrial DNA genomes included 13 protein-coding genes, 22 tRNA, two rRNA, one O(L)R, and one control region.

A Rab11a-enriched subapical membrane compartment regulates a cytoskeleton-dependent transcytotic pathway in secretory epithelial cells of the lacrimal gland.

Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathway in LGACs regulated by Rab11a for polymeric immunoglobulin receptor (pIgR) and dIgA. EGFP-tagged Rab11a expressed in primary LGACs labeled a unique membrane compartment of comparable localization to endogenous Rab11a beneath the apical plasma membrane.

Upregulation of hemoglobin expression by oxidative stress in hepatocytes and its implication in nonalcoholic steatohepatitis.

Recent studies revealed that hemoglobin is expressed in some non-erythrocytes and it suppresses oxidative stress when overexpressed. Oxidative stress plays a critical role in the pathogenesis of non-alcoholic steatohepatitis (NASH). This study was designed to investigate whether hemoglobin is expressed in hepatocytes and how it is related to oxidative stress in NASH patients.

Acid secretion-associated translocation of KCNJ15 in gastric parietal cells.

Potassium ions are required for gastric acid secretion. Several potassium channels have been implicated in providing K(+) at the apical membrane of parietal cells. In examining the mRNA expression levels between gastric mucosa and liver tissue, KCNJ15 stood out as the most highly specific K(+) channel in the gastric mucosa.

Phylogenetic evaluation of the taxonomic status of Papilio maackii and P. syfanius (Lepidoptera: Papilionidae).

The taxonomic status of Papilio maackii and P. syfanius has long been disputed. We conducted a molecular phylogenetic study to evaluate the taxonomic status of P.

[Pullout test in expansive unilateral open-door laminoplasty of the cervical spine with OsteoMed M3 plate and screws: the screw orientation matters].

Objective: To study the impact of screw orientation on the pullout strength of OsteoMed M3 titanium screws in expansive unilateral open-door laminoplasty of the cervical spine.

Methods: Six fresh human cervical spine specimens were randomly numbered and OsteoMed M3 plate and screws were used for an expansive unilateral open-door laminoplasty. The screws were inserted in the lateral mass at different extraversion angles (0°, 30° and 45°).

[Expression and clinical significance of caudal type homeobox transcription factor-2 in adult acute lymphocytic leukemia].

This study was aimed to investigate the expression and clinical significance of CDX1, CDX2 and CDX4 genes in acute lymphocytic leukemia (ALL). Expressions of CDX1, CDX2, and CDX4 in 51 adult acute lymphocytic leukemia patients and 14 healthy subjects were detected by reverse transcription polymerase chain reaction (RT-PCR). The results indicated that CDX1, CDX2 and CDX4 were not expressed in 14 healthy persons and 15 CR ALL patients, the positive expression rate of CDX2 gene in de novo ALL patients was 60.

Talin1, a valuable marker for diagnosis and prognostic assessment of human hepatocelluar carcinomas.

Background/aims: Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent cancers in the human population. Despite its significance, there is only limited understanding of pathological mechanisms and therapeutic options. Talin1, a focal adhesion complex protein that is required for cell adhesion and motility, regulates integrin interactions with extracellular matrix (ECM).

Phosphorylation of radixin regulates cell polarity and Mrp-2 distribution in hepatocytes.

Radixin, the dominant ezrin-radixin-moesin (ERM) protein in hepatocytes, has two important binding domains: an NH(2)-terminal region that binds to plasma membrane and a COOH-terminal region that binds to F-actin after a conformational activation by phosphorylation at Thr564. The present studies were undertaken to investigate the cellular changes in expression of radixin in WIF-B cells and to assess radixin distribution and its influence on cell polarity. We used a recombinant adenoviral expression system encoding radixin wild-type and Thr564 mutants fused to cyan fluorescent protein (CFP), as well as conventional immunostaining procedures.

Lipid in the livers of adolescents with nonalcoholic steatohepatitis: combined effects of pathways on steatosis.

Fatty liver is a prerequisite for the development of nonalcoholic steatohepatitis (NASH). The homeostasis of hepatic lipid is determined by the dynamic balance of multiple pathways introducing lipids into or removing lipids from hepatocytes. We aim to study the different contributions of major lipid pathways to fat deposition in NASH livers.

Penetrating cranionasal injury in a child caused by a bicycle spoke.

Penetrating cranionasal injuries are relatively rare, usually occur in young children, and can be caused by a variety of unusual objects. The mortality and disability rates are high without appropriate treatment. We report a penetrating cranionasal injury caused by a bicycle spoke.

Rab27b localizes to the tubulovesicle membranes of gastric parietal cells and regulates acid secretion.

Background & Aims: Rabs are monomeric guanosine triphosphatases that regulate membrane trafficking and acid secretion in gastric parietal cells. Using a proteomics approach, we identified a new Rab, Rab27b, in tubulovesicle membranes and determined its role in parietal cell activation.

Methods: We used mass spectrometry (MS) to identify Rab27b in purified tubulovesicular membrane fractions and used immunoblot and immunofluorescence analyses to study its expression.

[Utilization of functional neck dissection for treatment of recurrent branchial cleft anomalies].

Objective: To evaluate the utilization of functional neck dissection for treatment of recurrent branchial cleft anomalies.

Method: Fifteen patients with recurrent branchial cleft anomalies (fistula and cyst) undergoing functional neck dissection were retrospectively analyzed.

Result: Complications included 2 incisions secondary healing, 1 postoperative choking persisting for 1 months and 1 Horner's syndrome.

A possible mechanism for ezrin to establish epithelial cell polarity.

Ezrin is an important membrane/actin cytoskeleton linker protein, especially in epithelia. Ezrin has two important binding domains: an NH(2)-terminal region that binds to plasma membrane and a COOH-terminal region that binds to F-actin only after a conformational activation by phosphorylation at Thr567 of ezrin. The present experiments were undertaken to investigate the detailed cellular changes in the time course of expression of ezrin-T567 mutants (nonphosphorylatable T567A and permanent phospho-mimic T567D) in parietal cells and to assess ezrin distribution and its influence on the elaborate membrane recruitment processes of these cells.