[Effect of jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes].

Objective: To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I(Ca-L)) in ventricular myocytes of guinea pigs.

Methods: Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417.

Roles of adherence and matrix metalloproteinases in growth patterns of fungal pathogens in cornea.

Purpose: To investigate the roles of adherence and matrix metalloproteinases (MMPs) in growth patterns of major fungal pathogens in cornea.

Methods: Ninety-six eyes in 96 rabbits were equally divided into four groups receiving inoculation of fungal conidia of Aspergillus fumigatus, Candida albicans, Fusarium solani, and Penicillium citreo-viride, respectively, to induce fungal keratitis. Corneas in each group were obtained at 2, 8, 16 hr, and 1, 2, 3, 5, and 8 days after inoculation and were subjected to scanning electron microscopy, histopathological examination, and gelatin zymography.

[Effects of lung protective ventilation in prone or supine position on inhibiting adrenomedullin release in acute respiratory distress syndrome caused by pulmonary and extra-pulmonary insults in dog].

Objective: To investigate the changes in adrenomedullin release in bronchial alveolar lavage fluid (BALF) from different lung areas in acute respiratory distress syndrome (ARDS) of produced dogs, by pulmonary and extra-pulmonary causes, with low tidal volume and positive end expiratory pressure (PEEP) treatment under supine and prone position.

Methods: Twenty-four male mongrel dogs were randomly divided into ARDSp (ARDS caused by pulmonary causes) supine group, ARDSp prone group, ARDSexp (ARDS caused by extra-pulmonary causes) supine group, and ARDSexp prone group. A detergent to cause lung injury in ARDSp dogs, and intravenous oleic acid was given in ARDSexp dogs.

[Intraocular implantation of cyclosporine A drug delivery system in the treatment of experimental uveitis].

Objective: To investigate the effects and safety of cyclosporine A drug delivery system (CsA DDS) implanted into vitreous cavity on the treatment of experimental rabbit uveitis.

Method: A model of uveitis was established in 30 New Zealand white rabbits (30 eyes). The rabbits were randomized into control group (group A, 6 eyes), intravitreal non-medicated DDS group (group B, 6 eyes), oral CsA group (group C, 6 eyes) and intravitreal CsA DDS group (group D, 12 eyes).

[Evaluation of antifungal chemotherapeutic effects on fungal keratitis by confocal microscopy].

Objective: To evaluate the validity of confocal microscopy in estimating curative effect and in directing the treatment for fungal keratitis in the process of antifungal chemotherapy.

Methods: Fifty-eight patients, who were confirmed fungal infection by confocal microscopy, were selected from 328 patients with fungal keratitis. All patients received routine topical and/or oral antifungal medication, and were examined by confocal microscopy once a week and one week after discontinuation of the treatment.

[The surgical treatment for posterior capsule opacification in children with pseudophakic eyes].

Objective: To evaluate the surgical technique and outcome of pars plana capsulotomy and vitrectomy with infusion through the limbus to remove the posterior capsule opacification (PCO) in children with pseudophakic eyes.

Methods: This technique was performed in 51 cases (57 eyes) of complicated PCO with pseudophakic eyes, which was almost impossible to remove through the Nd:YAG capsulotomy. Pre- and post-operative visual acuity, intraocular pressure, density of cornea endothelium and complications during and after the operation were observed.

Follow-up study on pulmonary function and lung radiographic changes in rehabilitating severe acute respiratory syndrome patients after discharge.

Objectives: To follow-up on the changes in lung function and lung radiographic pictures of severe acute respiratory syndrome (SARS) patients discharged from Xiaotangshan Hospital in Beijing (by regularly receiving examination), and to analyze retrospectively the treatment strategy in these patients.

Methods: Surviving SARS patients were seen at least twice within 3 months after discharge and underwent SARS-associated coronavirus (SARS-CoV) IgG antibody testing, pulmonary function testing, and chest radiography and/or high-resolution CT (HRCT) examinations at Chinese PLA General Hospital. The treatments received at Xiaotangshan Hospital were analyzed retrospectively and were correlated to later status.

[Study on the dynamics of IgG antibody in 311 patients with severe acute respiratory syndrome].

Objective: To detect the level and dynamic change of severe acute respiratory syndrome (SARS)-coronavirus-specific IgG antibody in conavalescent SARS patients, and to provide information for prevention and vaccine development.

Methods: IgG antibody against coronavirus was detected by ELISA in the blood of 311 convalescent SARS patients for every 2 - 4 weeks. Stata 7.

[Combined penetrating keratoplasty with keratolimbal allograft transplantation in the treatment of severe corneal burns].

Objective: To study the clinical results of combined penetrating keratoplasty with keratolimbal allograft transplantation in the treatment of severe corneal burns.

Methods: The present study consisted of 35 eyes (34 patients) with full thickness corneal opacities and extensive corneal conjunctivalization after severe corneal burns. The duration between the injury and the time of presentation ranged from four months to 23 years.

[The corneal allograft rejection features in CD4 and CD8 knock-out mice].

Objective: To study the mechanism and the features of corneal allograft rejection using a model of CD4 and CD8 knock-out mice.

Methods: The mice were divided into three groups with 20 mice in each group. CD4 knock-out mice, CD8 knock-out mice and C57BL/6 mice were used as recipients and BALB/c mice as corneal graft donors.

[Refractive change and axial growth after bilateral intraocular lens implantation in children with congenital cataract].

Objective: To observe refractive change and axial growth in children with bilateral congenital cataract after intraocular lens (IOL) implantation.

Methods: 38 patients (76 eyes) with congenital cataract, who undergone cataract extraction, posterior continuous curvilinear capsulorhexis, anterior vitrectomy and primary IOL implantation in the capsular bag, were included. Patients were divided into four groups based on their age at surgery: 2 - 3 years (group A), 4 - 5 years (group B), 6 - 7 years (group C) and 8 - 12 years (group D).

[Inhibition of corneal neovascularization by liposomes mediated plasmid encoding human endostatin].

Objective: To evaluate the effect of liposomes mediated plasmid encoding endostatin (ES) for inhibiting experimental corneal neovascularization (CNV).

Methods: Thirty New Zealand albino rabbits were sutured on the superior cornea. Animals were randomly divided into 3 groups.

[Survey surgery delay for age-related cataract by a questionnaire in Qingdao].

Objective: To investigate the reasons why patients suffering hypermature or mature cataract in the urban Qingdao city do not undergo cataract surgery in time.

Methods: 104 inpatients with mature or hypermature age-related cataract were interviewed with a questionnaire concerning medical, economic, social and psychological factors.

Results: The factors related with the surgery delay were medical factor 44.

[Inhibition of proliferation of human lens epithelial cell line HLE-B3 by adenovirus-mediated transfer of antisense c-myc gene].

Objective: To investigate the effects of adenovirus-mediated transfer of antisense c-myc gene on proliferation and survival of human lens epithelial cell.

Methods: Immortalized human lens epithelial cell line (HLE-B3) was infected by replication-deficient adenovirus of antisense c-myc gene (Ad-AS-myc). The expression of c-myc mRNA and protein was detected by RT-PCR and Western blotting respectively.

[Clinical study on the endothelial immune rejection after penetrating keratoplasty].

Objective: To study the relationship between the grafts transparency and the endothelial immune rejection at different periods after penetrating keratoplasty (PKP).

Methods: A following-up was performed on patients received PKP in Shandong Eye Institute and Hospital between January 1994 and December 1998. A total of 648 cases (648 eyes) were involved in this study, including 444 males (444 eyes) and 204 females (204 eyes).

Inhibition of human lens epithelial B-3 cell proliferation by adenovirus-mediated transfer of antisense c-myc construct.

Purpose: To investigate the effects of adenovirus-mediated transfer of antisense c-myc construct on human lens epithelial B-3 (HLE B-3) cell proliferation, apoptosis and cell cycle.

Methods: HLE B-3 cell cultures were transduced with replication-defective adenovirus bearing either a nuclear-targeted beta-galactosidase (Ad-lacZ) or an antisense c-myc construct (Ad-AS-myc). The presence of beta-galactosidase activity in the transduced cultures was detected by immunohistochemical X-Gal staining, while c-myc mRNA and protein expression levels were evaluated by RT-PCR and Western blot analysis.

Dynamic changes of serum SARS-coronavirus IgG, pulmonary function and radiography in patients recovering from SARS after hospital discharge.

Objective: The intent of this study was to examine the recovery of individuals who had been hospitalized for severe acute respiratory syndrome (SARS) in the year following their discharge from the hospital. Parameters studied included serum levels of SARS coronavirus (SARS-CoV) IgG antibody, tests of lung function, and imaging data to evaluate changes in lung fibrosis. In addition, we explored the incidence of femoral head necrosis in some of the individuals recovering from SARS.

[Clinical study of the treatment of acanthamoeba keratitis by penetrating keratoplasty].

Objective: To evaluate the influence of accurate diagnosis and peri-operative management on the recurrence of acanthamoeba keratitis after penetrating keratoplasty.

Methods: A retrospective study of 18 cases of medically unresponsive keratitis in Shandong Eye Institute and Hospital that were confirmed as acanthamoeba keratitis based on laboratory examination or histopathological studies. The diagnosis of acanthamoeba keratitis was made prior to the surgery in 12 cases and delayed until histopathological results in six cases.

[Dynamic changes of immunoglobulin G in convalescents who have suffered from severe acute respiratory syndrome patients].

Objective: To investigate the dynamic changes of the antibody specific to severe acute respiratory syndrome (SARS) coronavirus in convalescents who have suffered from SARS.

Methods: Samples of peripheral blood were collected twice during the first 2 weeks after discharge and then once every 2 - 4 weeks from 310 convalescents of SARS, 131 males and 179 females, aged 18 - 74, discharged from hospitals in Beijing April 3 to June 20 2003 with the average discharge date of June 10, to detect the level of immunoglobulin G (IgG) by ELISA. A curve of level of IgG was drawn to describe the change by the specific month in the year.

[Organ culture for preservation of the cornea: human umbilical cord serum versus fetal bovine serum].

Objective: To evaluate the changes of porcine corneal endothelium, the morphology, histology, ultrastructure, enzymes activity and metabolism of the cornea induced by organ culture with two different media containing fetal bovine serum (FBS) or human umbilical cord serum (HCS).

Methods: Fifty pairs of porcine corneas were preserved at 31 degrees C for 7, 14, 21, 28 days. One cornea of each pair was cultivated in medium I containing 10% FBS (group 1); the other one was stored in medium II containing 10% HCS (group 2).

[Long-term effect of combined penetrating keratoplasty and pars plana vitrectomy with temporary keratoprosthesis].

Objective: To evaluate the long-term effect of combined penetrating keratoplasty, pars plana vitrectomy and temporary keratoprosthesis for the treatment of complicated anterior and posterior segment diseases.

Methods: Combined penetrating keratoplasty and pars plana vitrectomy were performed in 107 eyes with temporary keratoprosthesis. The mean follow-up period was 13 months.

[Effects of prone position ventilation on inflammatory factors in blood and bronchial alveolar lavage fluid of acute respiratory distress syndrome dogs caused by pulmonary and extrapulmonary insults].

Objective: To observe the changes of inflammatory factors in blood and bronchial alveolar lavage fluid (BALF) of different lung areas of acute respiratory distress syndrome (ARDS) dogs caused by pulmonary and extra-pulmonary causes with low tidal volume and positive end expiratory pressure (PEEP) treatment under prone position ventilation.

Methods: 24 male mongrel dogs were randomly divided into 4 equal groups:ARDSp (ARDS caused by pulmonary causes) experimental group, ARDSp control group, ARDSexp (ARDS caused by extra-pulmonary causes) experimental group, and ARDSexp control group. The ARDSp dogs inspired detergent to cause lung injury, and the ARDSexp dogs were injected with oleic acid through central vein.