Discovery and characterization of a novel alginate lyase PeAly15 and its truncated protein DUF4962 domain from Paenibacillus elgii HSFD1: Elucidation the molecular mechanism of endolytic mode.

Exolytic alginate lyases belonging to the PL15 family consist of two distinct domains: DUF4962 and Hepar_II_III. However, the specific functional roles of these domains have been scarcely investigated. In this study, we identified a novel alginate lyase, designated PeAly15, through comprehensive genomic analysis of the alginate-degrading bacterium Paenibacillus elgii HSFD1.

Optimization of the Cryoprotectants for Direct Vat Set Starters in Sichuan Paocai Using Response Surface Methodology.

The quality of Sichuan paocai in natural fermentation is often inconsistent due to the complexity of its microbial community and environmental influences. To address this, dominant microbial strains were selectively inoculated to improve the product's quality and safety. However, vacuum freeze-drying, commonly used to prepare direct vat set (DVS) starters, can significantly damage strains due to freezing stress.

Novel hybrid system based on carboxymethyl chitosan hydrogel encapsulating drug loaded nanoparticles for prolonged release of Vancomycin in the treatment of bacterial infection.

Current bacterial infections clinical treatments, such as intravenous antibiotic administration and local injection, suffer from short action duration, repeated administrations, and severe cell toxicity. To address these limitations, it is imperative to develop sustained drug release system with prolonged antimicrobial effects. In this work, a hybrid system was prepared using EDC/NHS catalyzed crosslinking-based carboxymethyl chitosan (CMCS) hydrogel as a carrier to encapsulate biodegradable nanoparticles (NPs) loaded with vancomycin, an efficient antibacterial drug.

Metal-organic cages based catalytic hybrid hydrogels for enhanced wound healing: Antibacterial and regenerative effects of Zr-MOC/chitosan composites hydrogel.

Metal-organic cages (MOCs), assembled by the coordination of metal nodes with organic ligands, offer excellent solvent dispersion, functionalization potential, and abundant binding sites, making them ideal for hybrid hydrogel synthesis. Herrin, a novel Zr-MOC/CS hybrid hydrogel was developed by crosslinking Zr-based metal-organic cages (Zr-MOC) and chitosan (CS) using dibenzaldehyde-functionalized polyethylene glycol (DF-PEG) as crosslinker, marking the first instance of incorporating Zr-MOC into a hydrogel matrix. The composite hydrogel leverages the catalytic activity of Zr-MOC to convert trace HO into hydroxyl radicals (·OH), delivering enhanced antibacterial performance.

New Insights on Hepatitis B Virus Viral Transcription in Single Hepatocytes.

The hepatitis B virus (HBV) infects approximately 290 million people globally, with chronic infection sustained by persistent viral gene expression. Recent single-cell analyses of HBV viral transcripts have uncovered novel features of HBV transcription and provided fresh insights into its regulation at the single-cell level. In this review, we summarize the latest advancements in understanding HBV viral transcription in individual hepatocytes and highlight emerging technologies that hold promise for future research.

Co-expression of α-L-rhamnosidase and β-glucosidase in Aspergillus niger and its application in enzymatic production of quercetin.

Quercetin (3,3',4',5,7-pentahydroxy flavonoid) is a bioactive flavonoid with significant medicinal properties. Similarly, Rutin (quercetin-3-O-rutinoside or 3',4',5,7-tetrahydroxy-flavone-3-rutinoside) is widely recognized flavonoid, and is converted to quercetin by α-L-rhamnosidase and β-D-glucosidase. In this study, engineered Aspergillus niger co-expressing α-L-rhamnosidase and β-glucosidase was successfully constructed, and engineered strain Rha1-BGL8 was obtained by enzyme activity assay.

Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice.

The α-L-rhamnosidase (rha1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047ΔpyrG, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α-L-rhamnosidase activities of 20.

Biochemical Properties of a Novel Cold-Adapted GH19 Chitinase with Three Chitin-Binding Domains from CSC-1 and Its Potential in Biocontrol of Plant Pathogenic Fungi.

GH19 (glycoside hydrolase 19) chitinases play crucial roles in the enzymatic conversion of chitin and biocontrol of phytopathogenic fungi. Herein, a novel multifunctional chitinase of GH19 (Chi19A), which contains three chitin-binding domains (ChBDs), was successfully cloned from CSC-1 and heterologously expressed in . We also generated truncated mutants of Chi19A_ΔI, Chi19A_ΔIΔII, and Chi19A_CatD consisting of two ChBDs and a catalytic domain, one ChBD and a catalytic domain, and only a catalytic domain, respectively.

Double-network polyphenol chitosan hydrogels with instant aldehyde-β-cyclodextrin-based structure as potential for treating bacterially infected wounds.

Treatment of multiple bacterial infected wounds by eliminating bacteria and promoting tissue regeneration remains a clinical challenge. Herein, dual-network hydrogels (CS-GA/A-β-CD) with snap-structure were designed to achieve curcumin immobilization, using gallic acid-grafted chitosan (CS-GA) and aldehyde-β-cyclodextrin (A-β-CD) crosslinked. A-β-CD were able to achieve rapid dissolution (≥222.

Heterologous Expression and Biochemical Characterization of a Novel Lytic Polysaccharide Monooxygenase from CSC-1.

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of recalcitrant polysaccharides. There are limited reports on LPMOs capable of concurrently catalyzing the oxidative cleavage of both cellulose and chitin. In this study, we identified and cloned a novel LPMO from the newly isolated bacterium CSC-1, designated as LPMO10.

The Discovery, Molecular Cloning, and Characterization of Dextransucrase DexA and Its Active Truncated Mutant from NN710.

Dextransucrases play a crucial role in the production of dextran from economical sucrose; therefore, there is a pressing demand to explore novel dextransucrases with better performance. This study characterized a dextransucrase enzyme, DexA, which was identified from the NN710. This bacterium was isolated from the soil of growing dragon fruit in Guangxi province, China.

Heterodimerization domains in MAP4 KINASEs determine subcellular localization and activity in Arabidopsis.

Signal transduction relies largely on the activity of kinases and phosphatases that control protein phosphorylation. However, we still know very little about phosphorylation-mediated signaling networks. Plant MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASEs (MAP4Ks) have recently gained more attention, given their role in a wide range of processes, including developmental processes and stress signaling.

Decoupled electrolysis for hydrogen production and hydrazine oxidation via high-capacity and stable pre-protonated vanadium hexacyanoferrate.

Decoupled electrolysis for hydrogen production with the aid of a redox mediator enables two half-reactions operating at different rates, time, and spaces, which offers great flexibility in operation. Herein, a pre-protonated vanadium hexacyanoferrate (p-VHCF) redox mediator is synthesized. It offers a high reversible specific capacity up to 128 mAh g and long cycling performance of 6000 cycles with capacity retention about 100% at a current density of 10 A g due to the enhanced hydrogen bonding network.

Bifidobacterial GH146 β-L-arabinofuranosidase for the removal of β1,3-L-arabinofuranosides on plant glycans.

L-Arabinofuranosides with β-linkages are present in several plant molecules, such as arabinogalactan proteins (AGPs), extensin, arabinan, and rhamnogalacturonan-II. We previously characterized a β-L-arabinofuranosidase from Bifidobacterium longum subsp. longum JCM 1217, Bll1HypBA1, which was found to belong to the glycoside hydrolase (GH) family 127.

A novel bi-functional cold-adaptive chitinase from Chitinilyticum aquatile CSC-1 for efficient synthesis of N-acetyl-D-glucosaminidase.

In order to better utilize chitinolytic enzymes to produce high-value N-acetyl-D-glucosamine (GlcNAc) from chitinous waste, there is an urgent need to explore bi-functional chitinases with exceptional properties of temperature, pH and metal tolerance. In this study, we cloned and characterized a novel bi-functional cold-adaptive chitinase called CaChi18A from a newly isolated strain, Chitinilyticum aquatile CSC-1, in Bama longevity village of Guangxi Province, China. The activity of CaChi18A at 50 °C was 4.

Effects of maize straw and its biochar application on soil organic carbon chemical composition and carbon degradation genes in a Moso bamboo forest.

We investigated the effects of maize straw and its biochar application on soil organic carbon chemical composition, the abundance of carbon degradation genes (I) and the composition of I gene community in a Moso bamboo forest, to provide the theoretical and scientific basis for enhancing carbon sequestration. We conducted a one-year field experiment in a subtropical Moso bamboo forest with three treatments: control (0 t C·hm), maize straw (5 t C·hm), and maize straw biochar (5 t C·hm). Soil samples were collected at the 3 and 12 months after the treatment.

Correction: Silver complexes with substituted terpyridines as promising anticancer metallodrugs and their crystal structure, photoluminescence, and DNA interactions.

Correction for 'Silver complexes with substituted terpyridines as promising anticancer metallodrugs and their crystal structure, photoluminescence, and DNA interactions' by Jiahe Li , , 2023, , 9607-9621, https://doi.org/10.1039/D2DT03463H.

Silver complexes with substituted terpyridines as promising anticancer metallodrugs and their crystal structure, photoluminescence, and DNA interactions.

Six silver hexafluoroantimonate complexes (1-6) with 4'-(4'-substituted-phenyl)-2,2':6',2''-terpyridine compounds bearing hydrogen (L1), methyl (L2), methylsulfonyl (L3), chloro (L4), bromo (L5) and iodo (L6) were prepared and characterized by H NMR, C NMR, IR, elemental analysis and single crystal X-ray diffraction. All the compounds exhibit interesting photoluminescence properties in the solid state and solution. data demonstrate that all of them show higher antiproliferative activities than cisplatin against three human carcinoma cell lines, A549, Eca-109 and MCF-7.

α-L-rhamnosidase: production, properties, and applications.

α-L-rhamnosidase [EC 3.2.1.

Discovery of a photoactivatable dimerized STING agonist based on the benzo[]selenophene scaffold.

Stimulator of interferon genes (STING) agonism presents a powerful weapon for cancer immunotherapy. This study reports a novel dimerized STING agonist diBSP01, which exhibited promising STING binding and activation properties , based on the benzo[]selenophene scaffold. Meanwhile, shielding the pharmacophores of diBSP01 with photoremovable protecting groups (PPGs) resulted in the generation of the first photoactivatable STING agonist, caged-diBSP01, that exerted no biological potency in the absence of light stimulation while regaining its STING agonistic activity after 400 nm irradiation.

Synthesis, characterization and anticancer properties: A series of highly selective palladium(II) substituted-terpyridine complexes.

Ten new palladium(II) complexes [PdCl(L)]Cl have been synthesized by the reaction of palladium(II) chloride and ten 4'-(substituted-phenyl)-2,2':6',2''-terpyridine ligands bearing hydrogen(L), p-hydroxyl(L), m-hydroxyl (L), o-hydroxyl (L), methyl (L), phenyl (L), fluoro (L), chloro (L), bromo (L), or iodo (L). Their structures were confirmed by FT-IR, H NMR, elemental analysis and/or single crystal X-ray diffraction analysis. Their in vitro anticancer activities were investigated based on five cell lines, including four cancer cell lines (A549, Eca-109, Bel-7402, MCF-7) and one normal cell line (HL-7702).

Expression and Purification of Glycosyltransferase DnmS from ATCC 27952 and Study on Catalytic Characterization of Its Reverse Glycosyltransferase Reaction.

Anthracyclines are an important class of natural antitumor drugs. They have a conservative aromatic tetracycline backbone that is substituted with different deoxyglucoses. The deoxyglucoses are crucial for the biological activity of many bacterial natural products after the proper modification from glycosyltransferases (GTs).