A Rapid Field-Visualization Detection Platform for Genetically Modified Soybean 'Zhonghuang 6106' Based on RPA-CRISPR.

Genetically modified (GM) herbicide-tolerant soybean 'Zhonghuang 6106', which introduces a glyphosate-resistant gene, ensures soybean yield while allowing farmers to reduce the use of other herbicides, thereby reducing weed management costs. To protect consumer rights and facilitate government supervision, we have established a simple and rapid on-site nucleic acid detection method for GM soybean 'Zhonghuang 6106'. This method leverages the isothermal amplification characteristics of RPA technology and the high specificity of CRISPR-Cas12a to achieve high sensitivity and accuracy in detecting GM soybean components.

Directional Adaptive Triboelectric Nanogenerator with Wind-Wave Synergy.

To enhance the adaptability and synergy of the triboelectric nanogenerator (TENG) in a wave environment, this paper introduces a directional adaptive triboelectric nanogenerator (DA-TENG) with wind-water synergistic action for wave energy collection. An innovative design combining a wind vane on the top and fan-shaped blade electrodes internally allows the DA-TENG to adjust its swinging direction adaptively to align with the direction of wave motion. The internal multiple power generation units work in coordination, effectively addressing the issues of low efficiency associated with spherical TENGs in capturing multidirectional wave energy.

Energy Storage Triboelectric Nanogenerator Based on Ratchet Mechanism for Random Ocean Energy Harvesting.

The instability of the ocean waves, such as intermittence, randomness, and irregularity, greatly affects the application of a triboelectric nanogenerator (TENG) in its aspects and leads to the irregularity and uncontrollability of its output performance. Hence, the energy storage TENG (ES-TENG) based on the ratchet mechanism is proposed in this work. The ES-TENG uses the ratchet mechanism to store the wave energy in the clockwork spring and then releases it in a centralized way to convert the wave energy into electric energy.

Proteomic and Metabolomic Evaluation of Insect- and Herbicide-Resistant Maize Seeds.

Label-free quantitative proteomic (LFQ) and widely targeted metabolomic analyses were applied in the safety evaluation of three genetically modified (GM) maize varieties, BBL, BFL-1, and BFL-2, in addition to their corresponding non-GM parent maize. A total of 76, 40, and 25 differentially expressed proteins (DEPs) were screened out in BBL, BFL-1, and BFL-2, respectively, and their abundance compared was with that in their non-GM parents. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the DEPs participate in biosynthesis of secondary metabolites, biosynthesis of amino acids, and metabolic pathways.

Analytical method for the out-of-plane buckling of the telescopic boom with guy cables.

The guy cables can effectively improve the bearing capacity for the telescopic boom of the crane, while the introduction of tensioned cables makes the buckling analysis complicated. The primary objective of this study was to propose an analytical method for the out-of-plane buckling of the telescopic boom with the spatial symmetric guy cables. To analyze the influence of the guy cables on the out-of-plane buckling property of the telescopic boom, the deflection differential equation of the multi-stepped telescopic boom with guy cables was established based on the theory of elastic beam, then the buckling characteristic equation to determine the critical load of the telescopic boom was derived.

Adrenomedullin ameliorates podocyte injury induced by puromycin aminonucleoside in vitro and in vivo through modulation of Rho GTPases.

Purpose: Podocyte injury is a key event in proteinuric kidney disease and eventually glomerular scarring. While adrenomedullin (AM), a potent vasodilatory peptide, has been reported to confer renoprotection in several experimental models of kidney diseases, its effect on injured podocytes and the related mechanism is still largely unknown.

Methods: Employing Western blotting analysis, immunoprecipitation and immunofluorescence, we investigated the effects of AM on the expressions of podocyte cytoskeletal proteins and Rho-family small GTPases (Rho GTPases) in puromycin aminonucleoside (PAN)-induced podocyte injury, both in cultured podocytes and in PAN nephrosis rats.