Synergistic effect of supercritical CO and organic solvent on exfoliation of graphene: experiment and atomistic simulation studies.

In this work, experiments and molecular dynamics (MD) simulations are carried out to explore the synergistic effect of supercritical CO2 (scCO2) and organic solvent on intercalation and exfoliation of graphene. Experimental characterizations via transmission electron microscopy, atomic force microscopy and Raman spectroscopy indicate that by combining scCO2 and organic solvent (N-methylpyrrolidone, NMP), few-layer graphene is successfully exfoliated from graphite, among which over 30% is 1-4 layers, and 55% is 5-8 layers. Systematic experiments have shown that compared with pure scCO2 or NMP, the mixed scCO2 and NMP can significantly increase the amount of graphene and the rate of few-layer graphene, and the optimum volume fraction of NMP is 25%.

Knockdown and Melatonin Supplementation in Embryo Development: Involvement of Mitochondrial Function and DNA Methylation.

In addition to pineal gland, many cells, tissues, and organs also synthesize melatonin (-acetyl-5-methoxytryptamine). Embryos are a group of special cells and whether they can synthesize melatonin is still an open question. However, melatonin application promoted embryo development in many species in condition.

Responses of Transgenic Melatonin-Enriched Goats on LPS Stimulation and the Proteogenomic Profiles of Their PBMCs.

The anti-inflammatory activity of melatonin (MT) has been well documented; however, little is known regarding endogenously occurring MT in this respect, especially for large animals. In the current study, we created a MT-enriched animal model (goats) overexpressing the MT synthetase gene . The responses of these animals to lipopolysaccharide (LPS) stimulation were systematically studied.