High-transition-temperature paraffin integration in IFAST device for efficient and robust nucleic acid extraction and detection.

Infectious diseases are prevalent in resource-limited regions with restricted access to health care. Nucleic acid testing is the gold standard for pathogen diagnosis. However, traditional methods are resource-intensive, which limits their use in point-of-care settings.

Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing.

Graphene field-effect transistors (GFETs) are widely used in biosensing due to their excellent properties in biomolecular signal amplification, exhibiting great potential for high-sensitivity and point-of-care testing in clinical diagnosis. However, difficulties in complicated fabrication steps are the main limitations for the further studies and applications of GFETs. In this study, a modular fabrication technique is introduced to construct microfluidic GFET biosensors within 3 independent steps.

Treatment Outcomes of Pulpectomy in Primary Maxillary Incisors Filled with ZOE and Metapex: A Two-year Retrospective Study.

Objectives: To address a literature gap by evaluating, in a larger set of samples, the clinical and radiographic outcomes of pulpectomy in primary maxillary incisors using ZOE and calcium hydroxide/iodoform paste. To also identify the predisposing factors for treatment outcomes.

Study Design: Medical charts of 124 patients (aged 16 to 60 months) and radiographs of their incisors (309 incisors) were reviewed (128 with ZOE and 181 with Metapex).

A novel framework of collaborative early warning for COVID-19 based on blockchain and smart contracts.

Early warning is a vital component of emergency response systems for infectious diseases. However, most early warning systems are centralized and isolated, thus there are potential risks of single evidence bias and decision-making errors. In this paper, we tackle this issue via proposing a novel framework of collaborative early warning for COVID-19 based on blockchain and smart contracts, aiming to crowdsource early warning tasks to distributed channels including medical institutions, social organizations, and even individuals.

Postoperative dental morbidity in children following dental treatment under general anesthesia.

Background: General anesthesia has been widely used in pediatric dentistry in recent years. However, there remain concerns about potential postoperative dental morbidity. The goal of this study was to identify the frequency of postoperative dental morbidity and factors associated with such morbidity in children.

Salivary microbial counts and buffer capacity in children with acute lymphoblastic leukemia.

Purpose: The aim of this study was to evaluate the caries activity in children undergoing maintenance stage chemotherapy courses.

Methods: Forty-six children with acute lymphoblastic leukemia (ALL) were examined, the age ranged from 3 to 12 years with a mean age of 7(1/2) years. They were under maintenance stage chemotherapy at the Department of Pediatric Hematology of Chang-Gung Memorial Hospital, Taoyuan, Taiwan.

Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora.

Lactobacillus paracasei has been demonstrated to inhibit the growth of many pathogenic microbes such as Streptococcus mutans, in vitro. However, its clinical application remains unclear. Here, we examined whether a novel probiotic L.