Introduction: Selenium-enriched foxtail millet () represents a functional cereal with significant health benefits for humans. This study endeavors to examine the impact of foliar application of sodium selenite (NaSeO) on foxtail millet, specifically focusing on selenium (Se) accumulation and transportation within various plant tissues.
Methods: To unravel the molecular mechanisms governing selenium accumulation and transportation in foxtail millet, we conducted a comprehensive analysis of selenium content and transcriptome responses in foxtail millet spikelets across different days (3, 5, 7, and 12) under NaSeO treatment (200 μmol/L).