Morphological and molecular identification of Sarcocystis spp. from the sika deer (Cervus nippon), including two new species Sarcocystis frondea and Sarcocystis nipponi.

Diaphragm muscles of 25 sika deer (Cervus nippon) farmed in Lithuania were examined for sarcocysts of Sarcocystis species. Two new Sarcocystis species, Sarcocystis frondea and Sarcocystis nipponi, were observed using light microscopy (LM) and transmission electron microscopy (TEM) and characterized by 18S ribosomal DNA (rDNA) and subunit I of cytochrome c oxidase (cox1) sequence analyses. By LM, sarcocysts of S.

Sarcocystis entzerothi n. sp. from the European roe deer (Capreolus capreolus).

In the present study, we describe Sarcocystis entzerothi n. sp. from the European roe deer (Capreolus capreolus) based on the microscopical and DNA analysis.

Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania.

The diaphragm muscles of eight sika deer (Cervus nippon) bred in Lithuania were examined for Sarcocystis cysts. Two Sarcocystis species, Sarcocystis taeniata, which were previously reported in Canadian moose (Alces alces) and Argentinean red deer (Cervus elaphus), and Sarcocystis pilosa n. sp.

Molecular identification of Sarcocystis rileyi sporocysts in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) in Lithuania.

Despite the fact that Sarcocystis rileyi is one of the earliest described species of the genus Sarcocystis forming macrocysts in ducks, the life cycle of this species is still unknown in Europe. Sarcocystis spp. oocysts/sporocysts were observed in faeces of four of 23 (17.

Description of Sarcocystis lari sp. n. (Apicomplexa: Sarcocystidae) from the great black-backed gull, Larus marinus (Charadriiformes: Laridae), on the basis of cyst morphology and molecular data.

A morphological type of Sarcocystis cysts found in one of two examined great black-backed gull, Larus marinus (Linnaeus) (Laridae), is considered to represent a new species for which the name Sarcocystis lari sp. n. is proposed and its description is provided.

Molecular and morphological investigations of Sarcocystis corvusi sp. nov. from the jackdaw (Corvus monedula).

One type of sarcocyst was found in two of eight investigated jackdaws (Corvus monedula) and proposed as Sarcocystis corvusi sp. nov. By light microscope, cysts resembled a thick thread and were very long (the largest fragment found amounted to 6 mm) and relatively thin (up to 60 μm).

Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the order Anseriformes.

On the basis of the already published morphological, 18S rDNA, 28S rDNA data (Kutkienė et al., Parasitol Res 99:562-565, 2006; Parasitol Res 102:691-696, 2008; Parasitol Res 104:329-336, 2009), and ITS-1 region investigation results of sarcocysts presented in this paper, Sarcocystis albifronsi sp. nov.

Sarcocystis sp. from the herring gull (Larus argentatus) identity to Sarcocystis wobeseri based on cyst morphology and DNA results.

Having studied 11 herring gulls (Larus argentatus) Sarcocystis cysts were found in neck and leg muscles of 4 birds. One type of sarcocysts (cyst type I) that have a thin (∼1.0 μm), smooth, or slightly wavy cyst wall without clearly visible protrusions and small (6.

Identification of Sarcocystis rileyi from the mallard duck (Anas platyrhynchos) in Europe: cyst morphology and results of DNA analysis.

Macroscopic cysts of Sarcocystis in ducks were recorded in Europe, but they were not investigated in more detail. Results of light and electron microscopy as well as 18S rDNA, 28S rDNA and ITS-1 region sequences of Sarcocystis macrocysts isolated from naturally infected mallard duck (Anas platyrhynchos) from Lithuania are presented in this paper. According to ultrastructure results, macrocysts examined corresponds to S.

The mallard duck (Anas platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis).

Morphometric and DNA investigation results of Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis) and Sarcocystis sp.

Sarcocystis in the birds family Corvidae with description of Sarcocystis cornixi sp. nov. from the hooded crow (Corvus cornix).

Having studied 67 birds of six species of the family Corvidae, Sarcocystis cysts were found in 16 (23.9%) individuals belonging to three species. The highest prevalence of infection (35.

Sarcocystis sp. from the goldeneye (Bucephala clangula) and the mallard (Anas platyrhynchos): cyst morphology and ribosomal DNA analysis.

By light microscopy, cysts of Sarcocystis sp. (cyst type I) from the goldeneye (Bucephala clangula) seemed filamentous with a smooth and thin (<1 microm) cyst wall. Ultrastructurally, the cyst wall surface was irregular with minute undulations of the primary cyst wall.

Sarcocystis spp. in birds of the order Anseriformes.

Having studied 342 birds of 20 species of the order Anseriformes, we found Sarcocystis cysts in 100 individuals (29.2+/-2.5%) belonging to 15 species.