Publications by authors named "Liubov A Shkodenko"
Article Synopsis
- Rapid and accurate diagnosis of meningitis is crucial to avoid severe outcomes, leading to the development of a new diagnostic assay that doesn't require specialized equipment.
- The assay features dual-priming isothermal amplification (DAMP) with unique internal primers to minimize false results, using Brilliant Green dye for effective fluorescence detection.
- It effectively identifies major meningitis-causing pathogens in clinical samples, demonstrating high specificity and sensitivity, making it suitable for standalone use or integration into point-of-care testing systems.
Structural RNA is a challenging target for recognition by hybridization probes. This chapter addresses the recognition problem of RNA amplicons in samples obtained by multiplex nucleic acid sequence-based amplification (NASBA). The method describes the design of G-quadruplex binary (split) DNA peroxidase sensors that produces colorimetric signal upon recognition of NASBA amplicons.
View Article and Find Full Text PDFVisual detection of ssRNA and dsDNA amplicons was achieved at room temperature without the need for a probe-analyte annealing stage. This approach uses a DNA nanostructure equipped with two analyte-binding arms. Highly selective binding of the third arm leads to the formation of a G-quadruplex structure capable of changing the solution color.
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