Effect of PM2.5 air pollution on the global burden of neonatal diarrhea from 1990 to 2019.

The primary risk factor for global disease burden is prolonged exposure to particulate matter (PM) air pollution, which results in health problems and death. However, the global burden of diarrheal diseases attributable to PM2.5 remains unclear.

Bi-magnetic MnO@Ni core-shell binary superparticles: Self-assembly preparation and magnetic behaviors.

Binary superparticles formed by self-assembling two different types of nanoparticles may utilize the synergistic interactions and create advanced multifunctional materials. Bi-magnetic superparticles with a core-shell structure have unique properties due to their specific spatial configurations. Herein, we built MnO@Ni core-shell binary superparticles via an emulsion self-assembly technique.

The global, regional, and national burden of stomach cancer among adolescents and young adults in 204 countries and territories, 1990-2019: A population-based study.

Background: Stomach cancer is a significant health problem in many countries. But healthcare needs of adolescents and young adults (AYAs) stomach cancer patients have been historically neglected. An accurate appraisal of the burden of AYA stomach cancer is crucial to formulating effective preventive strategies.

Driver Gene Alterations in Malignant Progression of Gastric Cancer.

The identification of driver genes is of great importance in modern medical research. It is also an essential factor in the development of individualization and has a positive effect on understanding the causes of cancer. Gene mutations are the primary cause of the outcomes of the process of tumorigenesis.

Effect of ionic liquid assisted hydrothermal carbonization on the properties and gasification reactivity of hydrochar derived from eucalyptus.

This project studies the relationship between the carbonization temperature and ionic liquid (IL) ([Bmim]Cl and [Bmim]OAC) solution and its impact on the structure, properties and gasification reactivity of the hydrochar obtained from eucalyptus via hydrothermal carbonization (HTC). The structure of hydrochar was analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy while its gasification reactivity with air was measured by thermogravimetric analysis at 340 °C. Results showed that the reactivity of hydrochar prepared in the presence of IL was much higher than that prepared using water.

Inhibition of GKN2 Attenuates Acute Gastric Lesions Through the NLRP3 Inflammasome.

Acute gastric lesions are commonly seen in critically ill patients in the intensive care unit and can result in significant upper gastrointestinal bleeding. However, the signaling mechanisms that regulate this severe disease are still unclear. In this study, we explored the involvement of gastrokine 2 (GKN2) in the development of acute gastric lesions in mice.

Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer.

Notch refers to a set of genes encoding a family of transmembrane receptors in mammalian cells. Previous evidence indicated that Notch receptors were implicated in the onset of gastric cancer. However, there is little evidence on the different genetic expression patterns of the four Notch receptors and their values for patient prognosis.

GKN2 promotes oxidative stress-induced gastric cancer cell apoptosis via the Hsc70 pathway.

Background: The GKN2 is a secretory protein, whose levels decrease in gastric cancer. The present study aimed to investigate the expression, function and mechanism of action of GKN2 in gastric cancer.

Methods: Molecular biology assays were performed to elucidate the function and underlying mechanisms of GKN2 in gastric cancer under stress-induced condition in vivo and in vitro.

Fatty liver disease is associated with the severity of acute pancreatitis:A systematic review and meta-analysis.

Background: Fatty liver (FL) has been positively associated with the risk of acute pancreatitis (AP), but whether FL is associated with the severity of AP remains unknown. To this, a meta-analysis was conducted to assess the effect of FL on severity and outcomes of AP.

Method: We searched PubMed, EMBASE and the Cochrane library to identify all eligible studies (up to June 2017).

A five-miRNA signature predicts survival in gastric cancer using bioinformatics analysis.

Abnormal expression of miRNAs is critical for gastric cancer progression. Here, we aimed to identify the differential expression of miRNAs in normal and cancerous gastric tissues and build a nomogram for effectively predicting the survival of patients with gastric cancer. We used high-throughput miRNA data in The Cancer Genome Atlas (TCGA) database for this study.

Long-term outcomes of laparoscopic versus open gastrectomy for advanced gastric cancer: A large cohort study.

Background: The adequacy of laparoscopy-assisted gastrectomy (LAG) for advanced gastric cancer (AGC) treatment remains uncertain. There are few reports on the long-term outcomes of laparoscopic versus open gastrectomy (OG) in AGC using subgroups with adequate patient numbers; which may provide further evidence to validate the long-term efficacy of LAG in AGC.

Methods: A retrospective cohort study included 1198 consecutive AGC patients underwent LAG or OG between September 2005 and October 2012 was conducted to compare the long-term outcomes.

Global transcriptome‑wide analysis of the function of GDDR in acute gastric lesions.

Acute gastric lesions induced by stress are frequent occurrences in medical establishments. The gastric dramatic downrelated gene (GDDR) is a secreted protein, which is abundantly expressed in normal gastric epithelia and is significantly decreased in gastric cancer. In our previous study, it was found that GDDR aggravated stress‑induced acute gastric lesions.

Laparoscopic versus open gastrectomy for gastric cancer with serous invasion: long-term outcomes.

Background: Laparoscopy-assisted gastrectomy (LAG) has already been introduced for advanced gastric cancer (AGC). However, little is known as to whether LAG is also applicable for AGC with serous invasion given the possibility that malignant cell dissemination and local recurrence incidence might increase. This study was designed to evaluate the technical feasibility and oncological adequacy of LAG in comparison with open gastrectomy (OG) for AGC with serous invasion.

Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.

Methyl‑CpG‑binding protein 2 (MeCP2) is a transcriptional repressor that has been implicated in tumor onset and progression. Compared with normal and other tumorous tissue, MeCP2 is highly expressed in well‑differentiated adenocarcinoma and mucinous adenocarcinoma tissues, particularly at the invasion site of colorectal cancer tissues. The aim of the present study was to evaluate the potential of MeCP2 for use as a therapeutic target for human colorectal cancer.

The relationship between multiple clinicopathological features and nerve invasion in pancreatic cancer.

Background: Nerve invasion is a specific type of tumor expansion and characteristic manifestation of pancreatic cancer (PC), with an incidence rate ranging from 50% to 100%. It is an important prognostic factor for pancreatic cancer, and its early detection is helpful in the management of the disease. This study was undertaken to analyze retrospectively the relationship between neural invasion and multiple clinicopathological features and to provide evidences for clinicians in the management of neural invasion in patients with PC.

Evaluation of two inflammation-based prognostic scores in patients with resectable gallbladder carcinoma.

Background: Survival after surgery for gallbladder cancer is generally poor. A number of inflammation-based prognostic scores have been established to help predict survival after surgery for several types of cancer. The objective of this study was to analyze and compare the utility of two inflammation-based prognostic scores, the Glasgow prognostic score (GPS) and the neutrophil-to-lymphocyte ratio (NLR), for predicting survival in patients with gallbladder cancer after surgery with curative intent.

Effect of siRNA-mediated knockdown of eIF3c gene on survival of colon cancer cells.

Eukaryotic initiation factor subunit c (eIF3c) has been identified as an oncogene that is over-expressed in tumor cells and, therefore, is a potential therapeutic target for gene-based cancer treatment. This study was focused on investigating the effect of small interfering RNA (siRNA)-mediated eIF3c gene knockdown on colon cancer cell survival. The eIF3c gene was observed to be highly expressed in colon cancer cell models.

Single-incision laparoscopic cholecystectomy versus multi-incision laparoscopic cholecystectomy: a meta-analysis of randomized clinical trials.

Background: Single-incision laparoscopic cholecystectomy (SILC) is theoretically supposed to be associated with better cosmetic results and less surgical-site pain than multi-incision laparoscopic cholesystectomy (MILC). So far, several relevant randomized controlled trials (RCTs) have been reported, but the results are conflicting.

Materials And Methods: Meta-analysis was conducted with all the qualified RCTs comparing SILC with MILC.

[Implication of different expression of IL-2 mRNA and IL-10 mRNA in CD4(+)CD25(+)T cell induced immune tolerance of liver transplantation in rat].

Objective: To investigate the expression of tolerance-associated interleukin (IL)-2 mRNA and IL-10 mRNA in rats after allogeneic liver transplantation.

Methods: The experimental rats were randomly divided into 3 groups: acute rejection model group (group I), CD4(+)CD25(+)T cell treatment group (group II), donors of the two groups were Wistar while recipients were Sprague-Dawley (SD) rats, and group III as control group, both donors and recipients of this group were SD rats, with 12 rats in each group. Splenic lymphocyte of donor in group II were injected through vena dorsalis penis 7 days before liver transplantation; equal volume normal saline (NS) were injected in rats of group I and III.

Experimental study on operative methods of pancreaticojejunostomy with reference to anastomotic patency and postoperative pancreatic exocrine function.

Aim: To assess the patency of pancreaticoenterostomy and pancreatic exocrine function after three surgical methods.

Methods: A pig model of pancreatic ductal dilation was made by ligating the main pancreatic duct. After 4 wk ligation, a total of 36 piglets were divided randomly into four groups.

[Combination of CD4+ CD25+ regulatory T cell and costimulatory pathway blockade inhibits acute rejection after liver transplantation: experiment with rats].

Objective: To investigate the effect of CD4+ CD25+ regulatory T cell (Treg) combined with anti-CD154 mAb, a costimulatory pathway inhibitor, on acute rejection after liver transplantation.

Methods: CD4+ T cells were isolated from the spleen of a Lewis rat and labeled with CD25-PE antibody. Anti-PE microbeads were added to collect CD4+ CD25+ T cells.

Hypoxia inducible factor-1alpha mediates protective effects of ischemic preconditioning on ECV-304 endothelial cells.

Aim: To investigate whether hypoxia inducible factor-1alpha (HIF-1alpha) is linked to the protective effects of ischemic preconditioning (IP) on sinusoidal endothelial cells against ischemia/reperfusion injury.

Methods: Sinusoidal endothelial cell lines ECV-304 were cultured and divided into four groups: control group, cells were cultured in complete DMEM medium; cold anoxia/warm reoxygenation (A/R) group, cells were preserved in a 4 centigrade UW solution in a mixture of 95% N2 and 5% CO2 for 24 h; anoxia-preconditioning (APC) group, cells were treated with 4 cycles of short anoxia and reoxygenation before prolonged anoxia-preconditioning treatment; and anoxia-preconditioning and hypoxia inducible factor-1alpha (HIF-1alpha) inhibitor (I-HIF-1) group, cells were pretreated with 5 microm of HIF-1alpha inhibitor NS398 in DMEM medium before subjected to the same treatment as group APC. After the anoxia treatment, each group was reoxygenated in a mixture of 95% air and 5% CO2 incubator for 6 h.

[Effect of heme oxugenase-1 on delayed xenograft rejection: experiment of guinea pig-to-rat liver xenotransplantation].

Objective: To investigate the effects and mechanism of heme oxygenase-1 (HO-1) in liver xenotransplantation and mechanism thereof.

Methods: Thirty male guinea-pigs used as donors were injected intravenously with cobra venom factor (CVF) and then randomly divided into 3 groups 24 hours after: Group A injected intraperineally with NaCl, Group B injected intraperineally with cobalt-protoporphyrin (CoPP), heme oxygenase-1 inducer, and Group C injected intraperineally with CoPP and zinc protoporphyrin (ZnPP), HO-1 inhibitor zinc before their livers were harvested. Thirty male SD rats used as recipients underwent the above-mentioned treatment 24 hours before receiving the xenografts.

Establishment of a new pig model for auxiliary partial orthotopic liver transplantation.

Aim: To establish a new pig model for auxiliary partial orthotopic liver transplantation (APOLT).

Methods: The liver of the donor was removed from its body. The left lobe of the liver was resected in vivo and the right lobe was used as a graft.

[Preliminary experimental study on treatment of portal hypertension with auxiliary partial orthotopic liver transplantation].

Objective: To evaluate the therapeutic efficacy of auxiliary partial orthotopic liver transplantation (APOLT) on portal hypertension in liver cirrhosis with amelioration of portal vein congestion, changes in portal vein pressure and status of the graft.

Methods: The recipients were porcine model of biliary cirrhosis reproduced by ligation of the common bile duct. During transplantation, arterial blood pressure, central venous pressure were recorded.