Photochromic and luminescence properties of a hybrid compound based on indoline spiropyran of the coumarin type and azomethinocoumarin.

A novel hybrid compound whose molecule includes the photochromic spiropyran fragment and two fluorophores - coumarin and salicylideneimine - was synthesized by the reaction of two equivalents of 8-formyl-7-hydroxy-4-methylcoumarin with 5-amino-1,3,3-trimethyl-2-methyleneindoline. Its spectral-kinetic and fluorescence properties were studied in comparison with model compounds - 7-hydroxy-4-methyl-coumarin, spiropyran of the coumarin type, and azomethinocoumarin in toluene and ethanol. It was shown that the hybrid compound exhibited photochromic transformations in both solvents.

[DIRECT REVASCULIZATION OF THE MYOCARDIUM FROM LEFT-SIDE MINITHORACOTOMY - MODERN PERFORMANCE OF THE OPERATION NAMED AFTER V.I. KOLESOV].

The article provides the results of minimally invasive direct revasculization of myocardium at the period from 2012 to 2014. Given method was used in 49 interventions. There weren't any lethal cases or myocardial infarctions.

[Application of a device for determination of the transplant length during operation of the aorta-coronary bypass].

Determination of the necessary length of the bypass is one of the principal stages of operation of the aorta-coronary bypass. The greatest difficulty of the determination of the bypass length is the first-priority applying of proximal anastomoses. It requires the surgeon to have great experience, the operation to be longer.

[Specific features of the technique of performing coronary bypass operations on the beating heart].

The article presents a technique of performing coronary bypass operations on the beating heart used by the authors in 847 patients which is based on high thoracic epidural anesthesia, primary formation of proximal anastomoses of the aorto-coronary bypasses and specified succession of the formation of distal, stabilization of the local part of the heart in the field of placing distal anastomosis and access to the lateral and posterior surface of the heart with vacuum stabilizers, using blower-humidifier and intracoronary shunts.

[Experience of left ventricular assist device "Incor" as "bridge to recovery" implantation in patients with end stage congestive heart failure].

Left ventricular assist device "Incor" ("Berlinhear", Germany) implantation experience in patient with ischemic cardiomiopathy and severe congestive heart failure is presented. Left ventricular assist device implantation was followed by coronary artery bypass grafting simultaneously. Total assist time was 211 days.

[Significance of CRF and dopamine receptors in amygdala for reinforcing effects of opiates and opioids on self-stimulation of lateral hypothalamus in rats].

Bipolar electrodes were implanted in the lateral hypothalamus in a group of 44 Wistar male rats in order to study self-stimulation reaction in the Skinner box. Simultaneously, microcanules were implanted into the central nucleus of the amygdala to inject the drugs (1 microl per injection). The blockade of corticoliberin (CRF) receptors (astressin, 1 microg) or Na+influx currents (xycaine or lidocain 1 microg) by the intrastructural administration of drugs into the amygdala decreased self-stimulation reaction of the lateral hypothalamus in rats by 29-55%.

[Delayed behavioral and morphological consequences in activation of the stress-antistress system in early ontogeny stages in rats].

Wistar rat pups aged 4-10-17 days were injected intraperitoneally with corticoliberin (corticotrophin-releasing hormone, CRH) in doses of 0.5 - 1.0 - 2.

[Features of surgical treatment of ischemic heart disease in women].

The features of preoperative status, course of the perioperative period and immediate results of coronary shunting were compared and analyzed in 523 patients, 56 of them were women. In female patients there were more often factors negatively influencing the course of the postoperative period. No additional intraoperative risk factors were found.

[Pharmacodynamic metoclopramide (cerucal) test in differential diagnosis of hypoprolactinemic hypogonadism syndrome in women].

Roentgenology revealed pituitary macroadenoma in 13 of 67 women with hyperprolactinemia. Craniographic structural abnormalities of the sella turcica suggested pituitary microadenoma in 20 women. Metoclopramide (cerucal) test was performed in the patients and a control group of 9 healthy women.

[Hyperprolactinemia syndrome in men].

A total of 122 male subjects suffering from secretory infertility were examined. In 25 of them (20 percent) blood prolactin levels were above the norm. Measurements of basal prolactin levels and of its secretion in metoclopramide test helped distinguish two types of hyperprolactinemia syndrome, differing in the pattern of spermatogenesis disorders.

[Immunomorphological research on the formation of the extracellular matrix in epithelial cell cultures].

Formation of extracellular matrix structures in cultures of rat liver epithelial nontransformed cell line IAR2 was studied with antisera to fibronectin, laminin and type IV collagen by immunofluorescence and immunoelectron microscopy of platinum replicas. Fibronectin formed peripheral spots of variable size some of which outlined free cell edges, as well as fibrils located towards the center of single cells or of cellular islands. Similarly distributed structures were seen in isolated matrices.

[Monoclonal antibodies to the proteins of intermediate filaments and of basement membranes in the differential diagnosis of certain forms of human breast tumors].

Monoclonal antibodies to keratins No. 8 and 17 specific for lining epithelium and myoepithelium of the mammary gland, respectively, as well as to basement membrane laminin, entactin, collagen type IV and heparan sulfate proteoglycan were used to the immunohistochemical analysis of 77 benign and malignant human breast lesions and that of 38 cases in which an intraoperative biopsy diagnosis was difficult. Morphologically similar benign and malignant proliferations were distinguished by keratin expression.

[Dopaminergic regulation of the hypothalamo-hypophyseal system in patients with hypophyseal adenomas].

A study was made of the state of the hypothalamohypophyseal system in patients with different types of chromophobe adenoma. Altogether 62 patients were examined using metoclopramide and Parlodel tests (38 patients were with somatotropinoma, 13--with prolactinoma, 11--with somatotropinoma with hyperprolactinemia). The time course of STH, PRL and TSH secretion in the blood was investigated.

[Heterogeneity of basal membranes in human tissues detected by monoclonal antibodies to laminin and entactin].

The distribution of basal membrane glycoproteins, laminin and entactin, was studied immunohistochemically by peroxidase-antiperoxidase technique in different adult human organs: kidneys, liver, heart, skin, spleen and ileum. Monoclonal antibody against entactin (ELM2) reacted with all basal membranes. Monoclonal antibody against laminin (LT3.

[Process formation and dynamics of the spreading of cultured cells on an extracellular matrix of fibroblasts].

Flattening of the normal mouse and human fibroblasts and of the epithelial cells (lines from rat liver IAR-2, trachea of calf foetus FBT and mouse kidney MPTR) was studied on isolated extracellular matrix (EM) formed by human fibroblasts in culture has been studied. EM consisted of fibers, usually parallel to each other. Flattening of the fibroblasts on EM was slower and less even than on glass.

[Isolation and characteristics of the extracellular matrix of cultured cells].

Sodium deoxycholate extraction was used to isolate extracellular matrix from various cultured cells: human and murine embryonic fibroblasts, epithelial lines of mouse (MPTR), rat (IAR 2 and IAR 20), pig (SPEV) and cow (FBT). Protein composition of the matrix was studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunofluorescence. The matrix morphology was investigated by scanning electron microscopy.

[Fibronectin in cultured mouse sarcoma cells induced by foreign bodies].

Indirect fluorescence was used to study distribution of fibronectin in cultured cells of 5 mouse foreign body-induced sarcomas (PS-4, PS-84, PS-100, PS-103, PS-130) and cells of early (2-3 months postimplantation) capsules surrounding implanted plastic films. Accumulation of pericellular or matrix fibronectin in dense cultures compared to sparse ones was noted in all the lines except PS-84. In confluent cultures of these lines, a three-dimensional fibrillar network of fibronectin typical for fibroblast-like cells was observed.