A study on the impact of marital status on the survival status of prostate cancer patients based on propensity score matching.

Marital status is an independent prognostic factor for survival in many types of cancers, but its prognostic impact on patients with prostate cancer (PCa) has not been established. The aim of this study was to explore the independent prognostic factors of PCa and to investigate the effect of marital status on survival outcomes in patients with different stratified by PCa. Using the surveillance, epidemiology, and end results (SEER) database, we collected data on 584,655 PCa patients diagnosed between 1975 and 2019.

Studying the impact of marital status on diagnosis and survival prediction in pancreatic ductal carcinoma using machine learning methods.

Pancreatic cancer is a commonly occurring malignant tumor, with pancreatic ductal carcinoma (PDAC) accounting for approximately 95% of cases. According of its poor prognosis, identifying prognostic factors of pancreatic ductal carcinoma can provide physicians with a reliable theoretical foundation when predicting patient survival. This study aimed to analyze the impact of marital status on survival outcomes of PDAC patients using propensity score matching and machine learning.

Cuproptosis-related genes predict prognosis and trastuzumab therapeutic response in HER2-positive breast cancer.

Breast cancer is the most common diagnosed cancer, the HER2-positive subtype account for 15% of all breast cancer. HER2-targeted therapy is the mainstay treatment for HER2-positive breast cancer. Cuproptosis is a novel form of programmed cell death, and is caused by mitochondrial lipoylation and destabilization of iron-sulfur proteins triggered by copper, which was considered as a key player in various biological processes.

Nanozyme colorimetric sensing of L-cysteine and copper ions based on PtCo nanoparticles@multi-walled carbon nanotubes.

In this work, PtCo bimetallic nanoparticles supported on multi-walled carbon nanotubes (PtCo@MWCNTs) nanohybrid was prepared simply and used for the first time as a novel nanozyme in the colorimetric sensing of L-cysteine (L-Cys) and Cu. Due to its strong enzyme-like catalytic activity, the prepared PtCo@MWCNTs nanohybrid can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to form ox-TMB without HO. Interestingly, the oxidase-like active of PtCo@MWCNTs was effectively suppressed by L-Cys, which could reduce ox-TMB to colorless TMB and lead to a pronounced blue fading, and the absorbance at 652 nm gradually decreased with increasing L-Cys concentration.

Screening a DNA Aptamer Specifically Targeting Integrin β3 and Partially Inhibiting Tumor Cell Migration.

Due to its key roles in malignant tumor progression and reprograming of the tumor microenvironment, integrin β3 has attracted great attention as a new target for tumor therapy. However, the structure-function relationship of integrins β3 remains incompletely understood, leading to the shortage of specific and effective targeting probes. This work uses a purified extracellular domain of integrin β3 and integrin β3-positive cells to screen aptamers, specifically targeting integrin β3 in the native conformation on live cells through the SELEX approach.

Identification of Core Genes Shared by Endometrial Cancer and Ovarian Cancer Using an Integrated Approach.

Ovarian cancer (OC) is one of the most commonly diagnosed cancers among women. Moreover, Endometrial cancer (EC) is a usual genital tract cancer in females; however, the hub genes and molecular pathways shared by these two cancers have not been surveyed yet. So, this study aimed to identify the common candidate genes or biomarkers and molecular pathways in OC and EC.

DPP6 and MFAP5 are associated with immune infiltration as diagnostic biomarkers in distinguishing uterine leiomyosarcoma from leiomyoma.

Objective: Uterine leiomyosarcoma (ULMS) is the most common subtype of uterine sarcoma and is difficult to discern from uterine leiomyoma (ULM) preoperatively. The aim of the study was to determine the potential and significance of immunerelated diagnostic biomarkers in distinguishing ULMS from ULM.

Methods: Two public gene expression profiles (GSE36610 and GSE64763) from the GEO datasets containing ULMS and ULM samples were downloaded.

Protective effects of upregulated gene against the apoptosis of human retinal pigment epithelial cells .

Aim: To investigate the protective effect of heme oxygenase-1 (HO-1) against HO-induced apoptosis in human ARPE-19 cells.

Methods: The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by HO. Functional experiments including cell counting kit-8 (CCK-8) assay, flow cytometry (FCM) and mitochondrial membrane potential assay were conducted.

Zinc protects sperm from being damaged by reactive oxygen species in assisted reproduction techniques.

The aim of this study was to explore the effect of zinc on hydrogen peroxide-induced sperm damage in assisted reproduction techniques. First, sperms were selected from semen samples of 20 healthy men prepared by density gradient centrifugation. Selected sperm were treated with either 0.

[Genetic diagnosis of thalassemia mutations with free fetal DNA in pregnant plasma].

This research was aimed to develop a simple, rapid, accurate and non-invasive method by means of flow-through hybridization technology, which can be used for molecular screening and early prenatal diagnosis for detecting common β-thalassemias mutational genotypes. By using PCR technology combined with flow-through hybridization of low-density gene chip technology, the 6 sets of PCR primer single tube multiplex PCR system and 29 types of DNA probes were designed, then the mutational thalassemias in foetus DNA was rapidly detected in total of 60 anaemia pregnant women plasma. The results showed that 4 cases with deletional α-thalassemias, 3 cases with β-thalassemias, 1 case with mixed type of α & β-thalassemias were detected in foetus DNA of 60 pregnant women plasmas.