[Preparation and characterization of anti-Drosophila Dnop5 antibody].

Aim: To prepare the rabbit antibody against Dnop5 and identify its specificity.

Methods: Dnop5 cDNA was amplified by RT-PCR, and then was subcloned into the fusion expression vectors pET28a(+). After being expressed in E.

Heat shock protein 90 indirectly regulates ERK activity by affecting Raf protein metabolism.

Extracellular signal-regulated protein kinase (ERK) has been implicated in the pathogenesis of several nerve system diseases. As more and more kinases have been discovered to be the client proteins of the molecular chaperone Hsp90, the use of Hsp90 inhibitors to reduce abnormal kinase activity is a new treatment strategy for nerve system diseases. This study investigated the regulation of the ERK pathway by Hsp90.

[Preparation and specificity analysis of anti-Drosophila Dxl6 antibody].

In order to study the function of Dxl6 which is a novel member of SR protein family, its cDNA was cloned by RT-PCR, and the sequences of its RS domain and its middle part were subcloned into two fusion express vectors, pGEX-4T-1His(6)C and pET32a. After expressing in E.coli BL21, the truncated proteins of Dxl6 RS domain part and Dxl6 middle part in pGEX-4T-1His(6)C were purified and used to immunize rabbits.

[Expression analysis of mouse homologous proteins with human aldose reductase like-1].

Objective: To detect expression of mouse ARL-1 homologous proteins in mouse tissues, and analyze homology, genetic distance and phylogenetic relationship between human aldose reductase like-1 (ARL-1) and mouse homologous proteins.

Methods: Homology of mouse ARL-1 homologous proteins with human ARL-1 was analyzed by software Clustal X 1.8 using GenBank and Swiss-Prot database; genetic distance and phylogenetic relationship between mouse ARL-1 homologous proteins and human ARL-1 were analyzed by software Mega 2.

Preparation and characterization of polyclonal antibodies against ARL-1 protein.

Aim: To prepare and characterize polyclonal antibodies against aldose reductase-like (ARL-1) protein.

Methods: ARL-1 gene was inserted into the E. coli expression vector pGEX-4T-1(His)(6)C and vector pQE-30.

Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli.

Enterokinase (EC 3.4.21.