[Optimization of Supercritical Fluid Extraction of Total Flavonoids and Monomer Compositions from Taxus Remainder Extracts Free of Taxoids].

Objective: To optimize the supercritical fluid extraction technology of flavonoids from taxus remainder extracts free of taxoids( TREFT) by response surface methodology( RSM).

Methods: By using a central composite design( CCD) in four factors and five levels to optimize the extraction parameters. The effects of extraction time, temperature, pressure and different concentration of ethanol and their interaction on the extraction rate of total flavonoids, amentoflavone, quercetin and ginkgetin which extracted from TREFT by supercritical fluid CO2 were investigated.

Optimization of process parameters of extraction of amentoflavone, quercetin and ginkgetin from Taxus chinensis using supercritical CO2 plus co-solvent.

The effects of extraction time, temperature, pressure and different concentration of ethanol and their interactions on the yields of amentoflavone, quercetin and ginkgetin extracted from Taxus chinensis by supercritical CO2 were investigated by using a central composite design (CCD). An CCD experimental design with four factors and five levels was used to optimize the extraction parameters. Ultra performance liquid chromatography (UPLC) was used to analyze the content of the tree components in the extracts.

Separation and purification of flavonoid from Taxus remainder extracts free of taxoids using polystyrene and polyamide resin.

An efficient separation process of flavonoid from Taxus wallichiana var. mairei remainder extracts free of taxoids was developed in this study. AB-8 macroporous resin and polyamide resin offered the fine adsorption capacity, and its adsorption rate at 30°C fitted well to the Langmuir and Freundich isotherms.