Prediction of Long Non-Coding RNAs Based on Deep Learning.

With the rapid development of high-throughput sequencing technology, a large number of transcript sequences have been discovered, and how to identify long non-coding RNAs (lncRNAs) from transcripts is a challenging task. The identification and inclusion of lncRNAs not only can more clearly help us to understand life activities themselves, but can also help humans further explore and study the disease at the molecular level. At present, the detection of lncRNAs mainly includes two forms of calculation and experiment.

Usefulness of Positron Emission Tomography in Patients with Syphilis: A Systematic Review of Observational Studies.

Background: Diagnosis of syphilis is difficult. Follow-up and therapy evaluation of syphilitic patients are poor. Little is known about positron emission tomography (PET) in syphilis.

Restless Legs Syndrome in Adults in Peking Union Medical College Hospital.

Objective To investigate the clinical characteristics of restless legs syndrome (RLS) in adults in Peking Union Medical College Hospital and explore the sleep quality,fatigue degree,daytime sleepiness,disease severity,depression and anxiety of RLS patients.Methods Totally 4739 consecutive patients who visited the outpatient departments with any sleep complaint or leg discomforts were recruited in the study. Patients under 18 years were excluded.

Clinical significance of TLR3 and TLR4 in peripheral blood mononuclear cells from children with Henoch-Schönlein purpura nephritis.

The aim of the present study was to investigate the expression levels and clinical significance of Toll-like receptor (TLR) 3 and 4 in peripheral blood mononuclear cells (PBMCs) collected from children with Henoch-Schönlein purpura (HSP) nephritis. The randomized controlled trial was conducted between August 2011 and March 2013, and 105 children with a clinical diagnosis of HSP were enrolled in the study. According to the 24-h urinary protein measurements and the presence of renal damage, the 105 cases were divided into groups A, B and C as follows: Group A, children with HSP but without renal damage; group B, children with HSP nephritis but without proteinuria; group C, children with HSP nephritis and proteinuria.

[Prevalence of REM sleep behavior disorder in patients with brainstem lesions].

Objective: To explore the prevalence of REM sleep behavior disorder (RBD) and define the brainstem structures likely to be involved in its pathogenesis in patients with brainstem lesions on magnetic resonance imaging (MRI).

Methods: Sixty-two patients with definite brainstems lesions on brain MRI were recruited from Neurology Department, Peking Union Medical College Hospital. And 66 gender-and-age-matched healthy subjects were included as controls.

Mutations in PRRT2 result in paroxysmal dyskinesias with marked variability in clinical expression.

Background: Paroxysmal dyskinesias (PDs), a clinically and genetically heterogeneous group of episodic movement disorders, include kinesigenic PD (PKD), exercise-induced PD (PED) and non-kinesigenic PD (PNKD). These disorders are all transmitted as autosomal dominant traits with incomplete penetrance. Several PD-related genetic disorders, including PKD and familial infantile convulsions with paroxysmal choreoathetosis (ICCA), mapped to the same region on chromosome 16.

Serum fatty acid profiles using GC-MS and multivariate statistical analysis: potential biomarkers of Alzheimer's disease.

Previous studies showed the relationship between fatty acids and the risk of developing Alzheimer's disease (AD). However, they did not address potential differences in free fatty acid (FFA) profiles that could be used to distinguish between AD patients and healthy controls. In the present study we used gas chromatography-mass spectrometry (GC-MS) technology coupled with multivariate statistical analysis to study profiles of FFA in AD.

[The influence of rotary manipulation on the internal pressure of cervical nucleus pulposus].

Objective: To measure the pressure changes inside the cervical nucleus pulposus in fresh human cervical specimen by imitating different rotary manipulations.

Methods: The load of 100 N was applied for 5 second on the six fresh male cervical samples by using material test system, which imitated the human head weigh and the strength of cervical extensor muscle. After that, traction, rotation and pulling on the samples were performed in different sequence under the force of 150, 200, 300 N respectively.

[Etiological, clinical and imaging characters of reversible posterior encephalopathy syndrome].

Objective: To elucidate the clinical and imaging characters of reversible posterior encephalopathy syndrome (PRES) and to discuss the etiological factors and the probable pathogenesis.

Method: Retrospective analysis of basal diseases, clinical manifestations and imaging characters of 13 PRES patients was conducted with follow-ups.

Results: The common associated diseases are various kinds of kidney disease and renal inadequacy, eclampsia of gravidity and postpartum, connective tissue disease and immunosuppressives, et al.

[Inhibition effect of rhEPO on rhIL6-induced hepcidin mRNA expression in HepG2 cell lines and human primary hepatocytes].

This study was aimed to investigate the effect of rhIL-6 and rhEPO on hepcidin mRNA expression in HepG2 cells and human primary hepatocytes, and mechanism of rhEPO in treatment of anemia of chronic disease (ACD). The HepG2 cells and human primary hepatocytes were cultured with medium containing different concentrations of rhIL-6 and rhEPO for a certain time, then mRNA was isolated and its RT-PCR was performed, the bands were photographed and analyzed by UVI band, the hepcidin and G3PDH mRNA ratio were semi-quantitatively analyzed. The expression levels of hepcidin in GepG2 cells and human primary hepatocytes at different conditions were compared.

[Clinical characteristics of relapsing virus encephalitis and mechanisms of relapse].

Objective: To investigate the clinical characteristics of herpes simplex encephalitis (HSE) and to discuss the mechanism of its relapse.

Method: The clinical data of 6 patients with relapsing encephalitis, 4 male and 1 female, aged 14 - 49, out of 150 encephalitis cases were analyzed: 5 of them were suspected as with HSE clinically, and HSE was confirmed by pathology via biopsy in 2 of the 6 patients. The 5 patients were followed up for 2 - 6 years.

[Study on the correlation between hypertension and the indexes of vascular endothelial function among people living in the community].

Objective: To study the features of hypertension and vessel endothelium functional parameter in people living at the community level as well as the risk factors of hypertension. Differences of angiotensin II (Ang II ), prostacyclin (PGI2) and nitric oxide (NO) among normal group and three hypertension groups were also studied.

Methods: By cluster sampling, 1134 adult Han people were selected from the residential communities.

[Effects of lead exposure to rat placenta and pups during different gestation periods].

Objective: To investigate the effects of lead exposure to rat placenta and pups during different gestation periods.

Methods: All 108 Wistar rats (72 females, 36 males) were randomly divided into four groups. All rats were orally fed with 0.

Common genetic changes in hereditary and sporadic pituitary adenomas detected by comparative genomic hybridization.

Twenty-four pituitary adenomas, both the sporadic type (n = 18) and the type arising in association with either multiple endocrine neoplasia, type 1 (MEN1; n = 2), or Carney complex (CNC, n = 4) were analyzed by comparative genomic hybridization. Twenty-one (88%) tumors displayed chromosomal alterations. The number of chromosomal aberrations in each tumor varied from 2 to greater than 10.

[Blood motilin concentration and enteral nutrition in premature infants].

Objective: To study changes of plasma motilin concentration and it's effect on enteral nutrition in premature infants.

Methods: The plasma motilin concentration of 72 premature infants was measured within 12 hours after birth before enteral feeding and on day 3 and 7 of life by using radioimmunoassay. Sixteen full-term neonates were enrolled as controls.

[Diagnosis and misdiagnosis of adrenoleukodystrophy: a causal analysis].

Objective: To discuss the diagnosis of adrenoleukodystrophy(ALD) and analyse the causes of its misdiagnosis.

Methods: The clinical and laboratory data of six cases with ALD were analyzed.

Results: Among the six cases of ALD, 4 cases were of childhood cerebral ALD, 1 case of Addison only, and 1 case of adolescent cerebral ALD.

[The correlation between the electroencephalogram findings and clinical manifestations of Creutzfeldt-Jakob disease].

Objective: To investigate the correlation between the EEG findings and the clinical signs of Creutzfeldt-Jakob disease (CJD).

Methods: Serial standard recording during different stage of disease was conducted among 13 patients with CJD, 10 males and 3 females, with the year of onset of 58.2 (49 approximately 65).

[Cloning, expression and purification of neural specific HuD cDNA].

Objective: To prokaryoticly express and purify HuD protein and its RNA recognition motifs.

Methods: HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.