Effect of mitogen-activated protein kinase signal transduction pathway on multidrug resistance induced by vincristine in gastric cancer cell line MGC803.

Aim: To investigate the correlation between mitogen-activated protein kinase (MAPK) signal transduction pathway and multidrug resistance (MDR) in MGC803 cells.

Methods: Western blot was used to analyze the expression of MDR associated gene in transient vincristine (VCR) induced MGC803 cells, which were treated with or without the specific inhibitor of MAPK, PD098059. Morphologic analysis of the cells treated by VCR with or without PD098059 was determined by Wright-Giemsa staining.

Effect of staurosporine on cycle of human gastric cancer cells.

Aim: To study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.

Methods: Cell proliferation was evaluated by trypan blue dye exclusion method. Apoptotic morphology was observed under a transmission electron microscope.

[Preventive effects of ramosetron and granisetron in prevention of gastrointestinal reaction associated with chemotherapeutic agents: a comparative study].

Objective: To compare the efficacy and safety of ramosetron and granisetron in prevention of gastrointestinal reaction induced by cisplatin or adramycin.

Methods: Fifty chemotherapy-naive patients with histological confirmed malignancies were randomized divided into 2 groups: AB group (ramosetron 0.3 mg was given in first cycle and granisetron 3mg in the second cycle) and BA group (these drugs were given in the reverse sequence).

[Effect of staurosporine on cell cycle of human gastric cancer cells].

Background & Objective: Protein kinase C (PKC) has been considered to be a potentially suitable target for anticancer therapy. This study was designed to investigate the effect of PKC inhibitor staurosporine (ST) on the inhibition of proliferation, the induction of apoptosis, and the change of cell cycle in human gastric cancer cell lines MGC-803 and SGC-7901.

Methods: The inhibition rates of cell proliferation were evaluated by trypan blue exclusion.

WT1 downregulation during K562 cell differentiation and apoptosis induced by bufalin.

Objective: To study the change of WT1 gene expression during human leukemic K562 cell differentiation and apoptosis induced by bufalin.

Methods: Cell viability was determined by trypan blue exclusion, cell differentiation and apoptosis by nitro blue tetrazolium (NBT) reduction test, morphology and flow cytometry, expression of WT1 protein by Western blot analysis, and expression of WT1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results: (1) The cell proliferation was inhibited by bufalin and the IC(50) at 24, 48, 72 h were 0.

[Towards <>: idea, principle and methodology of design and research results].

<>is the first atlas with spatial analysis methods to study on plague distributions, epidemics, controls, foci characters and their relationships with environment in China. This paper briefly introduces the idea, principle and methodology of its design and the main research results from it. The atlas include ten map groups as general maps, environmental background maps, plague epidemic and foci background maps in the world, maps of plague foci and their environments, epidemic maps, distribution maps of reservoirs, distribution maps of vectors, biotype distribution maps of yersinia pestis, maps of control institutions and control result maps.