Skeletal muscle-specific Bambi deletion induces hypertrophy and oxidative switching coupling with adipocyte thermogenesis against metabolic disorders.

Skeletal muscle plays a significant role in both local and systemic energy metabolism. The current investigation aims to explore the role of the Bambi gene in skeletal muscle, focusing on its implications for muscle hypertrophy and systemic metabolism. We hypothesize that skeletal muscle-specific deletion of Bambi induces muscle hypertrophy, improves metabolic performance, and activates thermogenic adipocytes via the reprogramming of progenitor of iWAT, offering potential therapeutic strategies for metabolic syndromes.

Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification.

The current single-cell analysis technologies such as fluorescence-activated cell sorting (FACS) and fluorescence-activated droplet sorting (FADS) could decipher the cellular heterogeneity but were constrained by low sorting performance and cell viability. Here, an ultra-sensitive single-cell sorting platform has been developed by integrating the FADS technology with Tetramer-HCR-EvaGreen (THE) fluorescence signal amplification. The THE system produced much higher fluorescence signal than that of the single Tetramer or Tetramer-HCR signal amplification.

Dual modes of DNA N-methyladenine maintenance by distinct methyltransferase complexes.

Stable inheritance of DNA N-methyladenine (6mA) is crucial for its biological functions in eukaryotes. Here, we identify two distinct methyltransferase (MTase) complexes, both sharing the catalytic subunit AMT1, but featuring AMT6 and AMT7 as their unique components, respectively. While the two complexes are jointly responsible for 6mA maintenance methylation, they exhibit distinct enzymology, DNA/chromatin affinity, genomic distribution, and knockout phenotypes.

Shenlian Decoction Ameliorates LPS-Related Inflammation in db/db Mice: Coupling Network Pharmacology With Experimental Verification.

Shenlian (SL) decoction, a renowned traditional Chinese formula for diabetes mellitus, has also been employed to treat intestinal disorders. Previous studies have demonstrated the efficacy of SL decoction in regulating blood glucose and intestinal bacteria. Nevertheless, further analysis is required to elucidate the mechanistic link between SL decoction-mediated improvement of intestinal function and treatment of Type 2 diabetes mellitus (T2DM).

Optimal Timing and Approaches for First Tangential Excision in Patients with ≥70% TBSA Burns: Insights from a Retrospective Cohort of a Mass Casualty Incident.

Objective: We aim to determine the optimal timing and approaches for first tangential excision of severely burned patients ≥70% total body surface area (TBSA).

Background: Early tangential excision is the gold-standard surgical therapy for full-thickness burns. However, there are debates about its optimal timing and approaches for severely burned patients ≥70%TBSA.

Time-varying 3D optical torque via a single beam.

The spin angular momentum (SAM) plays a significant role in light-matter interactions. It is well known that light carrying SAM can exert optical torques on micro-objects and drive rotations, but 3D rotation around an arbitrary axis remains challenging. Here, we demonstrate full control of the 3D optical torque acting on a trapped microparticle by tailoring the vectorial SAM transfer.

Silencing of the plant-derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED.

Background: The whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability and reproductive capacity are linked to its ability to acquire and integrate horizontally transferred genes (HTGs) into its genome. These HTGs increase the physiological and metabolic capacities of this pest, including cholesterol synthesis, which is critical for its survival and reproductive success.

Exploring the Research Focus of RNA-Binding Proteins in Trauma and Burns.

Trauma and burns are leading causes of death and significant global health concerns. RNA-binding proteins (RBPs) play a crucial role in post-transcriptional gene regulation, influencing various biological processes of cellular RNAs. This study aims to review the emerging trends and key areas of research on RBPs in the context of trauma and burns.

Integrating ecological and recreational functions to optimize ecological security pattern in Fuzhou City.

The scientific establishment of the Ecological Security Pattern (ESP) is crucial for fostering the synergistic development of ecological and recreational functions, thereby enhancing urban ecological protection, recreational development, and sustainable growth. This study aimed to propose a novel method of constructing ESP considering both ecological and recreational functions, and to reconstruct ESP by weighing the relationship between ecological protection and recreational development. Utilizing Fuzhou City as a case study, a comprehensive application of methodologies including Morphological Spatial Pattern Analysis (MSPA), landscape connectivity analysis, ArcGIS spatial analysis, social network analysis (SNA), and circuit theory is employed to develop both the ESP and the Recreational Spatial Pattern (RSP).

Learning environment as a predictor for medical students' empathy: a multicenter cross-sectional study from 12 medical schools in China.

Background: Empathy of medical students is crucial, yet it tends to decline as students enter later academic years. Empathy appeared to be affected by the learning environment (LE), which could be a potential contributor. We conducted a cross-sectional study to investigate the association between LE and empathy.

Gene Deficiency of δ Subunit-Containing GABA Receptor in mPFC Lead Learning and Memory Impairment in Mice.

Maintaining GABAergic inhibition within physiological limits in the medial prefrontal cortex (mPFC) is critical for working memory. While synaptic GABAR typically mediate the primary component of mPFC inhibition, the role of extrasynaptic δ-GABAR in working memory remains unclear. To investigate this, we used fiber photometry to examine the effects of δ-GABAR in freely moving mice.

Impact of miRNAs involved in the STAT3 signaling pathway on esophageal cancer (Review).

Esophageal cancer (ESCA) is a common tumor noted in the digestive tract, which is highly malignant due to unclear early symptoms and poor last‑stage treatment effects; its mortality rate is relatively high. MicroRNA (miR) and signal transducer and activator of transcription 3 (STAT3) are key components of cellular signaling pathways; their interaction forms a complex and intricate information network that controls several types of biological behaviors in the cells. In the tumor cell, these signal transduction pathways are abnormally active, indicating that the STAT3 signaling pathway mediated by miRs is involved in the progression of various cancer types.

Environmental influence on single methylation variation sites (SMVs) in the large yellow croaker (Larimichthys crocea): identification and correlation analysis.

Background: Larimichthys crocea is an important aquaculture species along the southeastern coast of China, with diverse environment and farming practices since artificial breeding, these different aquatic habitats are subject to significant variations in environmental factors that may involve modulation of gene expression through epigenetic mechanisms to enable species to survive and reproduce.

Methods And Results: This study aimed to identify methylation variation sites (SMVs) in different sequence contexts (CG, CHG, and CHH) within populations of L. crocea in different habitats.

Using Data-Science Approaches to Unravel Insights for Enhanced Transport of Lithium Ions in Single-Ion Conducting Polymer Electrolytes.

Solid polymer electrolytes have yet to achieve the desired ionic conductivity (>1 mS/cm) near room temperature required for many applications. This target implies the need to reduce the effective energy barriers for ion transport in polymer electrolytes to around 20 kJ/mol. In this work, we combine information extracted from existing experimental results with theoretical calculations to provide insights into ion transport in single-ion conductors (SICs) with a focus on lithium ion SICs.

The effect of leadership styles on employee's performance in Ethiopian sports organizations with mediating and moderating roles.

The research explored the impacts of diverse leadership styles on employee performance in Ethiopian sports organizations. It specifically examined the mediating effects of job satisfaction and the moderating impact of education level. In this study a cross-sectional survey design was employed, with 463 participants from various sports organizations.

Construction and validation of a regulatory T cells-based classification of renal cell carcinoma: an integrated bioinformatic analysis and clinical cohort study.

Purpose: Renal cell carcinoma (RCC), exhibiting remarkable heterogeneity, can be highly infiltrated by regulatory T cells (Tregs). However, the relationship between Treg and the heterogeneity of RCC remains to be explored.

Methods: We acquired single-cell RNA-seq profiles and 537 bulk RNA-seq profiles of TCGA-KIRC cohort.

Revealing long-range heterogeneous organization of nucleoproteins with N-methyladenine footprinting.

A major challenge in epigenetics is uncovering the dynamic distribution of nucleosomes and other DNA-binding proteins, which plays a crucial role in regulating cellular functions. Established approaches such as ATAC-seq, ChIP-seq, and CUT&RUN provide valuable insights but are limited by the ensemble nature of their data, masking the cellular and molecular heterogeneity that is often functionally significant. Recently, long-read sequencing technologies, particularly Single Molecule, Real-Time (SMRT/PacBio) sequencing, have introduced transformative capabilities, such as N-methyladenine (6mA) footprinting.

Self-organizing human heart assembloids with autologous and developmentally relevant cardiac neural crest-derived tissues.

Unlabelled: Neural crest cells (NCCs) are a multipotent embryonic cell population of ectodermal origin that extensively migrate during early development and contribute to the formation of multiple tissues. Cardiac NCCs play a critical role in heart development by orchestrating outflow tract septation, valve formation, aortic arch artery patterning, parasympathetic innervation, and maturation of the cardiac conduction system. Abnormal migration, proliferation, or differentiation of cardiac NCCs can lead to severe congenital cardiovascular malformations.

Advanced solar photo-Fenton-like process with directly growing nano-heterojunctions on graphite fiber felt for phenolic wastewater treatment :Synergistically expand the pH activity range and facilitate the Fe(III)/Fe(II) cycle.

Nanoscale FeWO/BiVO heterojunctions were directly grown on the graphite fiber felt (GF) with good conductivity to construct a FeWO/BiVO @GF solar photo-Fenton like wastewater treatment system. The removal effect of COD from phenolic wastewater and the mechanism of synergistic improvement of wastewater treatment efficiency by this system were investigated. The FeWO/BiVO heterojunction prepared by hydrothermal method exhibited higher photoelectric conversion efficiency and solar light utilization rate, thus endowing FeWO/BiVO with excellent solar-Fenton like reaction activity.

Evaluation of functional magnetic resonance APT and DKI imaging for breast cancer.

Objective: This study aimed to compare the performance of amide proton transfer-weighted imaging (APTWI) and diffusion kurtosis imaging (DKI) in differentiating benign from malignant breast lesions, evaluate molecular subtypes of breast cancer, and determine the diagnostic efficacy of the quantitative magnetic resonance imaging (qMRI) parameters in differentiating benign from malignant breast diseases.

Methods: The study included 168 women who underwent breast APTWI and DKI at Yunnan Cancer Hospital between December 2022 and July 2023. The APT signal intensity (SI), apparent kurtosis coefficient (Kapp), non-Gaussian diffusion coefficient (Dapp), and apparent diffusion coefficient (ADC) values were measured before surgery.

Negative pressure wound therapy for the management of deep brain stimulation-related surgical site infections: A retrospective case series.

The management of deep brain stimulation (DBS)-related surgical site infection (SSI) is challenging. This article aimed to report the efficacy of negative pressure wound therapy (NPWT) in treating DBS-related SSI while preserving all DBS devices. As a retrospective case series in a single center, localized DBS-related SSI was treated with complete debridement and NPWT, with preserving all DBS devices.

Permeability-Engineered Compartmentation System-Enabled Digital PCR (PECS-dPCR): A Digital Platform toward Multistep Biomolecular Assays.

Droplet-based digital PCR has emerged as a powerful platform for nucleic acid-based detection. However, the formation of droplet compartments and the subsequent amplification process in oil present significant drawbacks: instability under harsh thermal conditions, high background fluorescent noise inside droplets, and major difficulty in supporting multistep assays. Alternatively, droplets made of a hydrogel, or other advanced materials, have been adopted and demonstrate promising improvement over conventional droplet-based platforms.

Modification of Uniaxial Stress-Strain Model of Concrete Confined by Pitting Corroded Stirrups.

To investigate the impact of stirrup pitting corrosion on the stress-strain model of core concrete under compression, this study, based on existing corroded steel specimens, establishes a probabilistic model of the residual cross-sectional area distribution of steel bars to reasonably evaluate the effect of pitting on the mechanical performance of stirrups. Considering the tension stiffening effect in reinforced concrete, a time-dependent damage model of corroded steel bars in concrete was determined, and the existing stress-strain model of concrete confined by stirrups was ultimately modified, establishing a time-dependent constitutive model that incorporates the effects of stirrup pitting corrosion. A comparison with previous experimental results indicates that the revised model presented in this paper can appropriately reflect the changes in the mechanical performance of concrete confined by corroded stirrups.