Molecular and biological characteristics of two rare bloodstream Candida isolates: Candida nonsorbophila and Candida sonorensis.

Background: The incidence of new infections caused by rare Candida species has been steadily increasing, particularly in immunocompromised patients. This study investigates two rare Candida species responsible for Candida bloodstream infections and explores their molecular characteristics.

Methods: Clinical Candida strains were continuously isolated from the lower respiratory tract and blood specimens of a patient.

Investigation of the Impact Factors and Efficacy of N-Butylphthalide (NBP) on Functional Outcomes Following Mechanical Thrombectomy in Stroke Patients.

Background: Ischemic stroke was a major cause of mortality and disability worldwide. Mechanical thrombectomy (MT) has improved acute ischemic stroke treatment by restoring blood flow in large vessel occlusions. Yet, reperfusion injury remains a challenge, necessitating adjunctive neuroprotective strategies to enhance recovery.

Thrombelastography and Serum Homer1 to Assess Hemorrhagic Transformation After Thrombolysis in Acute Ischemic Stroke.

Background: This study aimed to investigate the correlation between early thrombelastography (TEG) and serum Homer1 with hemorrhagic transformation (HT) after intravenous thrombolysis (IVH) in acute ischemic stroke (AIS).

Methods: This prospective cohort study was conducted from January 2021 to December 2023. TEG parameters and serum Homer1 levels were measured after IVH treatment.

Dynamic changes in 3D chromatin structure during male gametogenesis in Arabidopsis thaliana.

Background: Chromatin higher-order structure plays an important role in genome stability maintenance and gene transcriptional regulation; however, the dynamics of the three-dimensional (3D) chromatin in male gametophytes during the two rounds of mitosis remains elusive.

Results: Here, we use the optimized single-nucleus and low-input Hi-C methods to investigate changes in 3D chromatin structure in four types of male gametophyte nucleus at different stages. The reconstructed genome structures show that microspore nuclei develop towards two different directions.

Effects of probiotic supplementation on intestinal flora, brain-gut peptides and clinical outcomes in children with anorexia nervosa.

This study aims to assess the therapeutic effects of probiotic oral therapy in paediatric patients with anorexia nervosa (AN) and to investigate its impact on intestinal flora composition, brain-gut peptide levels and overall clinical outcomes. A retrospective study was conducted involving 100 children diagnosed with AN at Xingtang County People's Hospital between January 2023 and June 2024. Patients were divided into two groups: a control group ( 50) receiving zinc gluconate oral solution alone and an observation group ( 50) receiving zinc gluconate plus probiotics.

Molecular insights into the interplay between type 2 diabetes mellitus and osteoporosis: implications for endocrine health.

Background: Type 2 diabetes mellitus (T2DM) and osteoporosis are prevalent, interconnected chronic diseases that significantly impact global health. Understanding their complex biological relationship is crucial for improving patient outcomes and treatment strategies.

Method: This review examines recent research on the mechanisms linking T2DM with osteoporosis.

Exploring the therapeutic potential of small extracellular vesicles derived from induced pluripotent stem cell in periodontal regeneration.

Objectives: To investigate the role of small extracellular vesicles derived from induced pluripotent stem cells (iPSC-sEVs) in periodontal tissue regeneration, elucidate their potential molecular mechanisms, and provide theoretical guidance for the clinical application of iPSC-sEVs as a cell-free therapeutic strategy for periodontal tissue regeneration.

Methods: We investigated the effects of iPSC-sEVs on the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. The regenerative potential of iPSC-sEVs was evaluated in vivo, using a periodontal defect model.

Engineered Strategies to Interfere with Macrophage Fate in Myocardial Infarction.

Myocardial infarction (MI), a severe cardiovascular condition, is typically triggered by coronary artery disease, resulting in ischemic damage and the subsequent necrosis of the myocardium. Macrophages, known for their remarkable plasticity, are capable of exhibiting a range of phenotypes and functions as they react to diverse stimuli within their local microenvironment. In recent years, there has been an increasing number of studies on the regulation of macrophage behavior based on tissue engineering strategies, and its regulatory mechanisms deserve further investigation.

Association of long-term weight management pharmacotherapy with multiple health outcomes: an umbrella review and evidence map.

Background: Multiple meta-analyses (MAs) have demonstrated that six pharmacotherapies, including orlistat, liraglutide, phentermine/topiramate, naltrexone/bupropion, semaglutide, and tirzepatide, improve weight loss and weight maintenance. However, few studies have synthesized and evaluated the quality of this evidence.

Objective: To identify the relevant MAs of randomized clinical trials (RCTs) that explored the association between the six pharmacotherapies and obesity-related health outcomes and adverse events (AEs).

Transcriptome Analysis Reveals the Mechanism of Y0-C10-HSL on Biofilm Formation and Motility of .

() is a type of pathogen that takes advantage of opportunities to infect and form biofilm during infection. Inhibiting biofilm formation is a promising approach for the treatment of biofilm-related infections. Here, Y0-C10-HSL (N-cyclopentyl-n-decanamide) was designed, synthesized, and tested for its effect on biofilm formation, motility, and the () survival assay.

Altered zygotic gene expression caused by sperm with variants disrupts early embryonic development.

The precise mechanisms behind early embryonic arrest due to sperm-related factors and the most effective strategies are not yet fully understood. Here, we present two cases of male infertility linked to novel variants, associated with oligoasthenoteratozoospermia (OAT) and early embryonic arrest. To investigate the underlying mechanisms and promising therapeutic approaches, knock-in and knock-out mice were generated.

Exploring the Molecular Mechanisms of Fisetin in Treating Periodontitis Through Multiomics and Network Pharmacology.

Background: Periodontitis (PD) is a common chronic inflammatory oral disease that severely affects patients' quality of life. Fisetin has been shown to possess antioxidant and anti-inflammatory properties in various biological systems.

Methods: This study first identified the molecular targets of fisetin for PD through network pharmacology analysis.

Ga-DOTA-FAPI-04 and F-FDG PET/CT: a head-to-head comparison for peritoneal carcinomatosis diagnostic accuracy.

Purpose: The study aimed to compare the diagnostic accuracy of Ga-DOTA-FAPI-04 (Ga-FAPI) and F-FDG PET/CT for peritoneal carcinomatosis (PC) in patients with various types of cancer.

Methods: The study enrolled 113 patients with suspected peritoneal malignancy, each of whom underwent Ga-FAPI and F-FDG PET/CT scans. Lesions in all patients were confirmed through pathology or radiological follow-up.

Reproductive toxicity and molecular mechanisms of benzo[a]pyrene exposure on ovary, testis, and brood pouch of sex-role-reversed seahorses (Hippocampus erectus).

As a flagship taxa for marine ecosystems, seahorses possess a unique reproductive strategy of "male pregnancy". They are severely threatened by marine petroleum-based pollution but the molecular mechanism involved remains unclear. We evaluated the toxic effects and mechanisms of sub-acute exposure to benzo[a]pyrene (BaP), a representative polycyclic aromatic hydrocarbon (PAH), at three environmental related dosages (0.

Anisotropic conductive scaffolds for post-infarction cardiac repair.

Myocardial infarction (MI) remains one of the most common and lethal cardiovascular diseases (CVDs), leading to the deterioration of cardiac function due to myocardial cell necrosis and fibrous scar tissue formation. Myocardial infarction (MI) remains one of the most common and lethal cardiovascular diseases (CVDs), leading to the deterioration of cardiac function due to myocardial cell necrosis and fibrous scar tissue formation. After MI, the anisotropic structural properties of myocardial tissue are destroyed, and its mechanical and electrical microenvironment also undergoes a series of pathological changes, such as ventricular wall stiffness, abnormal contraction, conduction network disruption, and irregular electrical signal propagation, which may further induce myocardial remodeling and even lead to heart failure.

Hepatic TM6SF2 activates antitumour immunity to suppress metabolic dysfunction-associated steatotic liver disease-related hepatocellular carcinoma and boosts immunotherapy.

Background: Transmembrane 6 superfamily member 2 (TM6SF2) has a protective role against metabolic dysfunction-associated steatotic liver disease (MASLD).

Objective: We aim to investigate the mechanistic role and therapeutic potential of hepatic TM6SF2 in MASLD-related hepatocellular carcinoma (HCC).

Design: Hepatocyte-specific knockout ( ) mice were fed with high-fat/high-cholesterol (HFHC) diet or diethylnitrosamine plus HFHC diet to induce MASLD-HCC.

The increasing burden of group B from 2013 to 2023: a retrospective cohort study in Beijing, China.

Group B (GBS) is a leading pathogen responsible for fatal infections in newborns primarily due to vertical transmission from colonized mothers. Cases of invasive GBS infections in adults have also increased and attracted attention recently. To comprehensively understand the evolving burden of vaginal GBS carriage in pregnant and non-pregnant women, as well as the trends in invasive GBS diseases and antibiotic resistance in China, we conducted a retrospective study using data from a large tertiary hospital in Beijing from 2013 to 2023.

Effect of curcumin regulated memory Th7 cells in mice with DSS-induced colitis.

Abnormal activation or dysfunction of memory helper T (mTh) cells is closely associated with the development of ulcerative colitis (UC). Curcumin (Cur), the main component of turmeric, plays a critical role in the treatment of UC due to its favorable anti-inflammatory and immunomodulatory bioactivities. However, whether Cur modulates mTh7 cells to alleviate UC is unknown.

Adaptation for to hosts via insertion mutation in the accessory gene regulator gene: decreased virulence and enhanced persistence capacity.

Unlabelled: is an important human pathogen due to its vast array of virulence factors regulated by multiple regulatory mechanisms, including the accessory gene regulator. In this study, two . strains were simultaneously isolated from the blood of a febrile patient, belonging to the same clone, designated as 23H with a complete hemolytic phenotype, and 23B, exhibiting an incomplete hemolytic phenotype.

Identification and Functional Analysis of Three Genes in .

The Cleavage Stimulation Factor (CstF) complex, consisting of three subunits, is essential for the 3' end processing of precursor messenger RNA (pre-mRNA). In mammals, this complex includes CstF50, CstF64, and CstF77, named according to their molecular weights, and these proteins are conserved across many organisms. However, the functional roles of the three CstF genes (, , and ) in , a major rice pest, have not been fully explored.

Functional Analysis of CPSF30 in Using RNA Interference Reveals Its Essential Role in Development and Survival.

The brown planthopper () is a major pest threatening global rice production, significantly reducing yields annually. As increasingly develops resistance to conventional control methods, such as chemical pesticides, there is an urgent need for innovative and sustainable pest management strategies. Cleavage and Polyadenylation Specificity Factor 30 (CPSF30) is a key protein involved in mRNA 3' end processing, yet its function in remains poorly understood.

Macrophage-specific κ-OR knockout exacerbates inflammation in hypoxic pulmonary hypertension.

Hypoxic pulmonary hypertension (HPH), a prevalent subtype of pulmonary arterial hypertension, is characterized by pulmonary vasoconstriction (HPV) and vascular remodeling, accompanied by inflammatory responses. Recent in vivo studies have shown a critical role of the κ-opioid receptor (κ-OR) in modulating the aforementioned pathological processes. Specifically, macrophage-specific κ-OR-knockout models have shown inflammatory response exacerbation with pulmonary hypertension and vascular remodeling.

Integrating progressive screening strategy-based continuous wavelet transform with EfficientNetV2 for enhanced near-infrared spectroscopy.

Near-infrared (NIR) spectroscopy has gained wide acceptance across various fields as a result of advances in portable equipment that can record spectra on site or at production lines. Continuous wavelet transform (CWT) can transform traditional one-dimensional (1D) NIR spectra into more informative two-dimensional (2D) spectrograms, thus enhancing the analysis and interpretation of spectral information. This study introduces a high-efficiency 2D CWT-EfficientNetV2 regression model to optimize NIR spectroscopy applications.

The efficacy and active compounds of Chaihuang Qingyi Huoxue granule to Ameliorate intestinal mucosal barrier injury in rats with severe acute pancreatitis by suppressing the HMGB1/TLR4/NF-κB signaling pathway.

Intestinal mucosal barrier injury represents a critical complication of severe acute pancreatitis (SAP) without effective treatment. This study investigated the efficacy, underlying mechanism, and responsible active compounds of the traditional Chinese medicinal prescription Chaihuang Qingyi Huoxue granule (CHQY) in treating SAP-induced intestinal mucosal barrier injury. SAP was established in Sprague-Dawley rats via intra-pancreaticobiliary duct infusion of sodium taurocholate, followed by oral CHQY administration (3.

The Predictive Value of NLR, PLR and SII for SSNHL in Children and Young Adults.

Unlabelled: Sudden Sensorineural Hearing Loss is a common emergency in otolaryngology, with an increasing incidence among younger age groups. This study retrospectively analyzes peripheral blood inflammatory markers in SSNHL patients under 24 years-old to explore the potential prognostic value of the immune inflammatory response in young SSNHL patients. This retrospective analysis included 53 SSNHL patients who received treatment from January 2016 to December 2023.