Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances.

Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising and critical method of broadening enzymatic applications in biosensing, biocatalysis, and biomedical devices; however, it is considerably hampered by limited strategies. Here, a core-shell strategy involving a soft-core hexahistidine metal assembly (HmA) is innovatively developed and characterized with encapsulated enzymes (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), and cascade enzymes (CAT+GOx)) and hard porous shells (zeolitic imidazolate framework (ZIF), ZIF-8, ZIF-67, ZIF-90, calcium carbonate, and hydroxyapatite). The enzyme-friendly environment provided by the embedded HmA proves beneficial for enhanced catalytic activity, which is particularly effective in preserving fragile enzymes that will have been deactivated without the HmA core during the mineralization of porous shells.

Enhancing the Implant Osteointegration via Supramolecular Co-Assembly Coating with Early Immunomodulation and Cell Colonization.

Osteointegration, the effective coupling between an implant and bone tissue, is a highly intricate biological process. The initial stages of bone-related immunomodulation and cellular colonization play crucial roles, but have received limited attention. Herein, a novel supramolecular co-assembled coating of strontium (Sr)-doped metal polyphenol networks (MPN) modified with c(RGDfc) is developed and well-characterized, for eliciting an early immunomodulation and cellular colonization.

Gaps in knowledge and management of iron deficiency in heart failure: a nationwide survey of cardiologists in China.

Background: Heart failure (HF) guidelines recommend routine testing for iron deficiency (ID) and, for those with ID, intravenous iron if the left ventricular ejection fraction is <50%. Guideline adherence to these recommendations by cardiologists in China is unknown.

Methods And Results: An independent academic web-based survey was designed and distributed via social networks to cardiologists across China.

Facile synthesis of 2-sulfonylbenzothiazoles cascade SO-NCS addition and S-C(sp) coupling.

A new synthetic route to 2-sulfonylbenzothiazoles (BtsR) employing cascade SO-NCS nucleophilic addition and CuI-catalysed S-C(sp) coupling was developed. Structurally diverse BtsR molecules were readily afforded in high yields from the rapid and benign reaction of 2-iodoarylisothiocyanates with sodium sulfinates in DMSO, generally without the need for further column isolation.

Damage analysis and grading control technology of surrounding rock in high geo-stress roadway.

To investigate the failure mechanism and establish stability control methods for surrounding rock in high geo-stress roadways, this study incorporated the brittle failure characteristics of the surrounding rock mass into a unified strength criterion, developing an elastic-plastic theoretical model to represent rock damage and fracture. Using this model, analytical expressions for the damage rupture radius and stress field distribution are derived, leading to the proposal of a grading control method. The analysis of calculation examples reveals that an increase in stress, damage degree, and the brittleness coefficient of the rock mass results in the expansion of both the plastic damage and damage residual zones.

ALG5 downregulation inhibits osteogenesis and promotes adipogenesis by regulating the N-glycosylation of SLC6A9 in osteoporosis.

Osteoporosis is characterized by decreased bone mass and accumulation of adipocytes in the bone marrow. The mechanism underlying the imbalance between osteoblastogenesis and adipogenesis in bone marrow mesenchymal stem cells (BMSCs) remains unclear. We found that ALG5 was significantly downregulated in BMSCs from osteoporotic specimens.

Multi-DNAzymes cascade reaction mediated aptasensors for OTA detection based on the integration of autocatalytic Mg-dependent DNAzyme cleavage and entropy-driven circuit.

Ochratoxin A (OTA) is a compound of concern due to its potential health effects on humans. Detecting OTA in food is crucial for safeguarding public health. In this study, we fabricated a multi-DNAzyme cascade reaction-mediated colorimetric aptasensors for OTA detection, integrating autocatalytic Mg-dependent DNAzyme cleavage (MNAzyme) and an entropy-driven circuit.

Cancer Cells in Sleep Mode: Wake Them to Eliminate or Keep Them Asleep Forever?

Cancer cell dormancy is a critical phase in cancer development, wherein cancer cells exist in a latent state marked by temporary but reversible growth arrest. This dormancy phase contributes to anticancer drug resistance, cancer recurrence, and metastasis. Treatment strategies aimed at cancer dormancy can be categorized into two contradictory approaches: inducing cancer cells into a dormant state or eliminating dormant cells.

Achieving theranostic probes targeting BRD3/BRD4 for imaging and therapy of tumor.

The BET family proteins play pleiotropic roles in the tumorigenesis and growth of various human malignancies that have aroused great interests as the cancer therapeutic targets. Therefore, it's significant to develop labeling toolkits for the dynamic monitoring of BET family proteins in living tumor cells and tissue slices. In particular, there are few small-molecule fluorescent probes based on BET family proteins developed for real-time imaging of these proteins in tumor cells and tissues and treatment of breast cancer.

Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling.

Background: Endometrial cancer (EC) represents a serious health concern among women globally. Excessive activation of the protooncogene c-Myc (c-Myc) is associated with the proliferation and stemness of EC cells. Phosphocholine (PC), which is synthesized by choline kinase alpha (CHKA) catalysis, is upregulated in EC tumor tissues.

Proteinase K impact on anaerobic co-digestion of modified biodegradable plastic and food waste: Step-by-step analysis with microorganism.

This study was designed to explore the key impact of Proteinase K (PK) on every step of anaerobic co-digestion. The results of step-by-step experiments indicated that PK promoted the hydrolysis of biodegradable plastic by initiating self-hydrolysis reactions, directly promoting the hydrolysis step of anaerobic co-digestion. Subsequently, PK indirectly promoted the acidogenesis and acetogenesis steps by impacting the proliferation of acid-producing bacteria.

Ketogenic diet in treating sepsis-related acquired weakness: is it friend or foe?

Background: Sepsis is the body's extreme response to an infection leading to organ dysfunction. Sepsis-related acquired weakness (SAW), a critical illness closely related to metabolic disorders, is characterized by generalized sepsis-induced skeletal muscle weakness, mainly manifesting as symmetrical atrophy of respiratory and limb muscles. Muscle accounts for 40% of the body's total mass and is one of the major sites of glucose and energy absorption.

NcBRI1 positively regulate vascular development and promote biomass production in Neolamarckia cadamba.

Brassinosteroids (BRs) are essential phytohormones that play a crucial role in plant growth and development. However, our understanding of BR receptors and their functions in tree species is currently limited. In this study, we looked for potential BR receptor genes in the burflower-tree (Neolamarckia cadamba) genome.

A green, versatile, and facile strategy for anti-biofouling surface with ultra-high graft density polyethylene glycol.

Implantable catheters are susceptible to severe complications due to non-specific protein adhesion on their surfaces. Polyethylene glycol (PEG) coatings, the gold standard for resistance to non-specific protein adhesion, present a challenge in achieving high-density grafting, which significantly restricts their use as anti-biofouling coatings. Herein, we exploited the strong interaction between polyphenols (PCs) and polycations (K6-PEG) to graft PEG onto the surface of PC-Cu (A network of metal polyphenols composed of proanthocyanidins and metal copper ions, with expectation for the coating with excellent resistance to non-specific protein adhesion (PC-Cu@K6-PEG).

Green Synthesis of 2-Mercapto 5,6-Dihydro-4-1,3-Thiazines via Sequential C-S Couplings.

The six-membered N,S-heterocyclic 1,3-thiazines and their derivatives are widely acknowledged as pharmaceutical molecules with a wide range of biological activities. In this study, we developed a unique thiol-involved cascade reaction that enables the efficient construction of the 5,6-dihydro-4-1,3-thiazine scaffold through consecutive intermolecular thiol-isothiocyanate and intramolecular thiol-halogen click reactions. Structurally diverse 2-mercapto dihydrothiazines including three antitumour candidates of -dihydrothiazines were readily obtained in high yields from the readily available thiols and 3-chloroisothiocyanate in the green solvent EtOH/HO (1:1) using KCO (0.

Modulating the Interactions of Peptide-Polyphenol for Supramolecular Assembly Coatings with Controllable Kinetics and Multifunctionalities.

Polyphenols and peptides represent two fundamental building blocks in the kingdom of supramolecular assembly (SA) coatings, which have recently attracted considerable interest. Regulating the assembly kinetics of SA coatings is critical to controlling the performance of SA coatings, but this area is still in its infancy, especially in the SA coating of peptide-polyphenol. Herein, a library of oligopeptides with rich diversity, numerous polyphenols, and modulators are explored to reveal their roles in the formation and regulation of SA coating.

WAC Facilitates Mitophagy-mediated MSC Osteogenesis and New Bone Formation via Protecting PINK1 from Ubiquitination-Dependent Degradation.

Osteogenic differentiation of mesenchymal stem cells (MSCs) plays a pivotal role in the pathogenesis and treatment of bone-related conditions such as osteoporosis and bone regeneration. While the WW domain-containing coiled-coil adaptor (WAC) protein is primarily associated with transcriptional regulation and autophagy, its involvement in MSC osteogenesis remains unclear. Here, the data reveal that the levels of WAC are diminished in both osteoporosis patients and osteoporosis mouse models.

Versatile Mechanochemical Reactions Via Tailored Force Transmission in Mechanophores.

In polymer mechanochemistry, regulating the intrinsic mechanical reactivity of a mechanophore offers extensive opportunities in material science, enabling the development of hierarchical and multifunctional polymer-based materials. Recent advances have focused on innovating various types of mechanophores with inherent reactivity (e.g.

Deep joint learning diagnosis of Alzheimer's disease based on multimodal feature fusion.

Alzheimer's disease (AD) is an advanced and incurable neurodegenerative disease. Genetic variations are intrinsic etiological factors contributing to the abnormal expression of brain function and structure in AD patients. A new multimodal feature fusion called "magnetic resonance imaging (MRI)-p value" was proposed to construct 3D fusion images by introducing genes as a priori knowledge.

Five-Component [2 + 2 + 1 + 1] Tandem Benzannulation Leading to Multifunctionalized Aromatic Amines.

An unprecedented five-component [2 + 2 + 1 + 1] benzannulation strategy for regioselective assembly of densely functionalized aromatic amines from two ynals, two malononitriles, and sodium sulfinates is established. The benzannulation protocol enables the efficient installation of five substituents on a benzene ring via the formation of multiple chemical bonds in a single operation, providing various multifunctionalized aromatic primary amines in moderate to good yields. Additionally, three-component [3 + 2 + 1] cycloaddition of malononitriles, ynals, and NHSCN was also achieved to produce 2-amnopyridine derivatives with NHSCN serving as an ammonia surrogate.

Bortezomib induces cell apoptosis and increases the efficacy of αPD-1 in BCR::ABL T315I mutation CML by targeting UBE2Q1.

The BCR:ABL T315I mutation presents a significant challenge in the current management of Chronic Myeloid Leukemia (CML), highlighting the need to identify novel targets and drugs. In our study, we observed the elevated expression of UBE2Q1 in KBM5-T315I cells compared to KBM5 cells, where it interacted with DDX3, regulating its ubiquitination. Furthermore, we found that Bortezomib (BTZ) targeted UBE2Q1, reducing its protein level expression.

Environmental Factors Drive the Biogeographic Pattern of Root Endophytic Fungal Diversity in the Arid Regions of Northwest China.

subsp. Rousi (Abbrev. ) stands as a vital botanical asset in ameliorating the ecological landscape of the arid regions in Northwest China, where its rhizospheric microorganisms serve as linchpins in its growth and developmental dynamics.

Impact of age at HBsAg seroclearance on hepatic outcomes and life expectancy in men with chronic HBV infection based on multi-state modeling of the natural history.

Background: The effects of age at HBsAg seroclearance on clinical outcomes and survival in chronic hepatitis B (CHB) have not been adequately assessed. We evaluated the impact of age at HBsAg seroclearance on long-term outcomes, along with how coexisting factors modified risks and life expectancy in CHB patients.

Methods: We used multi-state modeling approach to examine transitions through the CHB continuum in a longitudinal cohort study of male civil servants recruited in 1989-1992.

The detrimental effect of rainforest conversion to rubber plantations on soil dissolved organic carbon and C: N stoichiometry, mediated by altered soil biogeochemistry.

Rainforest conversion into rubber (Hevea brasiliensis) plantations (RP) alters global carbon cycling and contributes to climate change. However, the impact of this widespread tropical land use change on various elements of the carbon cycle is poorly understood. Here, we aimed to investigate the impact of rainforest conversion into RP on soil-dissolved organic carbon (DOC), one of the most mobile organic matter (OM) in the terrestrial ecosystem that causes the transformation and migration of C.