Location-specific net water uptake and malignant cerebral edema in acute anterior circulation occlusion ischemic stroke.

Background And Purpose: Early identification of malignant cerebral edema (MCE) in patients with acute ischemic stroke is crucial for timely interventions. We aimed to identify regions critically associated with MCE using the Alberta Stroke Program Early Computed Tomography Score (ASPECTS) to evaluate the association between location-specific-net water uptake (NWU) and MCE.

Materials And Methods: This multicentre, retrospective cohort study included patients with acute ischemic stroke following large anterior circulation occlusion.

A carrier-free ultrasound-responsive polyphenol nanonetworks with enhanced sonodynamic-immunotherapy for synergistic therapy of breast cancer.

Sonodynamic therapy (SDT) is an efficient non-invasive strategy for treating breast cancer. However, the therapeutic efficacy of SDT is greatly limited by various defense mechanisms in the tumor microenvironment, particularly the overexpression of B-cell lymphoma-2 (Bcl-2). In this study, based on drug self-delivery systems, a carrier-free ultrasound-responsive polyphenol nanonetwork (GTC) was developed to enhance SDT by inhibiting Bcl-2.

A study on the variation of cytokine and electrolyte levels in rhesus macaques, cynomolgus monkeys, and Assamese macaques.

Background: Non-human primates (NPHs), such as rhesus macaques, cynomolgus monkeys, and Assamese macaques, play a crucial role in biomedical research. However, baseline cytokine and electrolyte data for these three species, particularly data stratified by age and sex, are limited. Therefore, the aim of this study was to establish and analyze age- and sex-specific cytokine and electrolyte profiles in these three species.

Effects of on Metabolites and Flavors in Synthetic Microbiota During Baijiu Fermentation.

The distinctive flavor and aroma of Chinese baijiu are closely linked to the microorganisms involved in the fermentation process. , a dominant species in the fermentation of Chinese baijiu, has become a prominent research focus. In this study, we selected well-characterized pure cultures of microorganisms to construct diverse chassis microflora.

Quantitative analysis of basal bone width as an index for immediate implant placement in the aesthetic zone: A retrospective study.

Purpose: The basal bone of the anterior maxilla plays a crucial role in maintaining primary stability of immediate implants. However, no consensus exists on the relationship between basal bone width and immediate implant success, unlike for basal bone length. This study presents a clinical scheme to predict the primary stability of immediate implants using the basal bone width.

Targeting ClpP: Unlocking a novel therapeutic approach of isochlorogenic acid A for methicillin-resistant Staphylococcus aureus-infected osteomyelitis.

A medical predicament has led to extensive drug resistance in methicillin-resistant Staphylococcus aureus (MRSA), and the complexity of treatment has increased exponentially with the induction of osteomyelitis. In view of the severe situation and the potential of bacterial antivirulence strategies, this study focused on the key virulence factor caseinolytic protease (ClpP) of S. aureus to identify new strategies against MRSA-induced osteomyelitis.

Room-temperature creation and conversion of individual skyrmion bags in magnetic multilayered disks.

Skyrmion bags, with arbitrary topological charge Q, have recently attracted much interest, since such high-Q topological systems could open a way for topological magnetism research and are promising for spintronic applications with high flexibility for information encoding. Investigation on room-temperature skyrmion bags in magnetic multilayered structures is essential for applications and remains unexplored so far. Here, we demonstrate room-temperature creation and manipulation of individual skyrmion bags in magnetic multilayered disks.

"Cation-Recognition" Effect of 2D Nanochannels in Graphene Oxide Membranes Intercalated with Ionic Liquid for High Desalination Performance.

Water and ion transport in nanochannels is crucial for membrane-based technology in biological systems. 2D materials, especially graphene oxide (GO), the most frequently used as the starting material, are ideal building blocks for developing synthetic membranes. However, the selective exclusion of small ions while maintaining in a pressured filtration process remains a challenge for GO membranes.

Exogenous Trehalose Assists in Resisting High-Temperature Stress Mainly by Activating Genes Rather than Entering Metabolism.

is a typical aroma-producing yeast in food brewing, but it has low heat resistance and poor proliferation ability at high temperature. Trehalose is generally considered to be a protective agent that helps stable yeast cells resist heat shock stress, but its functional mechanism for yeast cells in the adaptation period under heat stress is unclear. In this study, the physiological metabolism changes, specific gene transcription expression characteristics, and transcriptome differences of under different carbon sources under high-temperature stress (40 °C) were compared to explore the mechanism of trehalose inducing to recover and proliferate under high-temperature stress during the adaptation period.

Precision autofocus in optical microscopy with liquid lenses controlled by deep reinforcement learning.

Microscopic imaging is a critical tool in scientific research, biomedical studies, and engineering applications, with an urgent need for system miniaturization and rapid, precision autofocus techniques. However, traditional microscopes and autofocus methods face hardware limitations and slow software speeds in achieving this goal. In response, this paper proposes the implementation of an adaptive Liquid Lens Microscope System utilizing Deep Reinforcement Learning-based Autofocus (DRLAF).

Fabrication of Polydopamine-Coated High-Entropy MXene Nanosheets for Targeted Photothermal Anticancer Therapy.

Transition metal carbides, nitrides, and carbonitrides (MXenes) have emerged as a promising class of 2D materials that can be used for various applications. Recently, a new form of high-entropy MXenes has been reported, which contains an increased number of elemental species that can increase the configurational entropy and reduce the Gibbs free energy. The unique structure and composition lead to a range of intriguing and tunable characteristics.

Development of a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Beiji nairovirus.

Beiji nairovirus (BJNV) is a recently discovered tick-borne RNA virus associated with human febrile illness. This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the precise detection of BJNV, with a specific focus on assessing its effectiveness with clinical samples. The optimal molecular target was identified as the BJNV small (S) segment gene, and the ideal reaction conditions were established at 65 °C for 50 min.

Pathogenic spectrum of infective endocarditis and analysis of prognostic risk factors following surgical treatment in a tertiary hospital in China.

Background: This study aimed to summarize the pathogenic spectrum of infective endocarditis (IE) and analyze the risk factors for poor prognosis in surgical patients in a tertiary hospital in China.

Methods: We retrospectively included patients diagnosed with IE between January 2013 and January 2022. The pathogenic spectrum was summarized; the risk factors for early postoperative mortality and embolic events were analyzed using multivariate logistic regression.

The segmented flavivirus Alongshan virus reduces mitochondrial mass by degrading STAT2 to suppress the innate immune response.

Alongshan virus (ALSV) is a newly discovered pathogen in the family, characterized by a unique multi-segmented genome that is distantly related to the canonical flaviviruses. Understanding the pathogenic mechanism of this emerging segmented flavivirus is crucial for the development of effective intervention strategies. In this study, we demonstrate that ALSV can infect various mammalian cells and induce the expression of antiviral genes.

Causal effects of circulating inflammatory proteins on knee and hip osteoarthritis: A two sample Mendelian randomization study.

Introduction: Numerous circulating proteins are linked to the presence or severity of joint inflammation. However, traditional studies could not explain whether these protein biomarkers are proximate to disease progression.

Method: We conducted a study to explore the causal effects of 91 circulating inflammation-related proteins (CIPs) on knee osteoarthritis (KOA) and hip osteoarthritis (HOA), using two-sample Mendelian randomization (MR).

Reinforcement learning-based optimal tracking control for uncertain multi-agent systems with uncertain topological networks.

Recent decades, extensive applications exemplified in intelligent connected vehicles (ICVs) and unmanned aerial vehicles (UAVs) have emerged with the rapidly development of multi-agent systems (MASs). Inspired by these applications, the optimal tracking control problem for uncertain MASs under uncertain topological networks is addressed based on the theory of observer design and reinforcement learning (RL). Thus, an adaptive extended observer based on concurrent learning (CL) technique is designed to simultaneously estimate system states and unknown parameters, where unknown parameters estimated convergence is guaranteed in a relaxed persistence of excitation condition.

Risk factors for mortality in patients with sepsis on extracorporeal membrane oxygenation and/or continuous renal replacement therapy: a retrospective cohort study based on MIMIC-IV database.

Objective: This study aimed to identify risk factors for mortality in septic patients undergoing extracorporeal membrane oxygenation (ECMO) and/or continuous renal replacement therapy (CRRT).

Methods: Data from the MIMIC-IV database were retrospectively reviewed for 24,502 septic patients treated with ECMO or CRRT between 2008 and 2019. After applying inclusion and exclusion criteria, 70 patients receiving ECMO, 513 receiving CRRT, and 22 receiving both were included in the final analysis.

Nerve-Inspired Optical Waveguide Stretchable Sensor Fusing Wireless Transmission and AI Enabling Smart Tele-Healthcare.

Flexible strain monitoring of hand and joint muscle movement is recognized as an effective method for the diagnosis and rehabilitation of neurological diseases such as stroke and Parkinson's disease. However, balancing high sensitivity and large strain, improving wearing comfort, and solving the separation of diagnosis and treatment are important challenges for further building tele-healthcare systems. Herein, a hydrogel-based optical waveguide stretchable (HOWS) sensor is proposed in this paper.

Multi-protomics analysis identified host cellular pathways perturbed by tick-borne encephalitis virus infection.

Tick-borne encephalitis virus (TBEV) represents a pivotal tick-transmitted flavivirus responsible for severe neurological consequences in Europe and Asia. The emergence of TBEV genetic mutations and vaccine-breakthrough infections, along with the absence of effective vaccines and specific drugs for other tick-borne flaviviruses associated with severe encephalitis or hemorrhagic fever, underscores the urgent need for progress in understanding the pathogenesis and intervention strategies for TBEV and related flaviviruses. Here we elucidate cellular alterations in the proteome, phosphoproteome, and acetylproteome upon TBEV infection.

Enhancing Mechanical Durability and Long-Term Stability in Organic Solar Cells via Flexible Linker-Sequential Block Copolymerized Donors.

Multi-component copolymerized donors (MCDs) hold great promise for improving both the efficiency and mechanical robustness of flexible organic solar cells (f-OSCs) owing to their facile molecular tunability and advantageous one-pot copolymerization. However, despite the excellent crystallinity imparted by their highly conjugated polymer backbone, MCDs often struggle to retain photovoltaic performance under large external deformations, limiting their applicability in wearable devices. Herein, we developed a novel series of flexible linker-sequential block MCDs (Fs-MCDs), specifically PM6-Cl-b-D18-Cl-BTB, PM6-Cl-b-D18-Cl-BTH, and PM6-Cl-b-D18-Cl-BTD, by precisely incorporating flexible functional groups into the conjugated polymer skeleton.

Development of Human Monoclonal Antibodies With Broad Reactivity for Rabies Postexposure Prophylaxis.

Rabies is an acute lethal disease causing by the neurotropic virus rabies virus (RABV). Rabies immune globulin (RIG) as an indispensable component of rabies postexposure prophylaxis (PEP) always faces with great challenges in terms of costs, stability and safety. Our objective is to develop a novel and potential fully human monoclonal antibodies (mAbs) cocktail for the improvement of rabies PEP.

The Design and Adaptive Control of a Parallel Chambered Pneumatic Muscle-Driven Soft Hand Robot for Grasping Rehabilitation.

The widespread application of exoskeletons driven by soft actuators in motion assistance and medical rehabilitation has proven effective for patients who struggle with precise object grasping and suffer from insufficient hand strength due to strokes or other conditions. Repetitive passive flexion/extension exercises and active grasp training are known to aid in the restoration of motor nerve function. However, conventional pneumatic artificial muscles (PAMs) used for hand rehabilitation typically allow for bending in only one direction, thereby limiting multi-degree-of-freedom movements.

Task Allocation and Sequence Planning for Human-Robot Collaborative Disassembly of End-of-Life Products Using the Bees Algorithm.

Remanufacturing, which benefits the environment and saves resources, is attracting increasing attention. Disassembly is arguably the most critical step in the remanufacturing of end-of-life (EoL) products. Human-robot collaborative disassembly as a flexible semi-automated approach can increase productivity and relieve people of tedious, laborious, and sometimes hazardous jobs.

Multi-proteomics and interactome dataset of tick-borne encephalitis virus infected host cells.

Tick-borne encephalitis virus (TBEV) is a significant viral pathogen transmitted by ticks, causing severe neurological complications in humans across Europe and Asia, highlighting the urgent need for an in-depth understanding of molecular functions of viral proteins and their interactions with the host proteome. Multi-omics analysis of how TBEV hijack cellular processes provides information about their replication and pathogenic mechanisms. Here, we focused on the proteome, phosphoproteome, and acetylproteome of Vero cells infected by TBEV, revealing the host perturbations triggered by TBEV infection.