N-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement.

Nitric oxide (NO) is a crucial signaling molecule that regulates a wide range of metabolic pathways in different strata of organisms. In plants, nitrate reductase (NR) is a key enzyme for NO biosynthesis. There are two NR-encoding genes in Arabidopsis genome, NIA1 and NIA2, which are precisely regulated and expressed in a tissue-specific manner.

A Novel Antigen Design Strategy to Isolate Single-Domain Antibodies that Target Human Nav1.7 and Reduce Pain in Animal Models.

Genetic studies have identified the voltage-gated sodium channel 1.7 (Na1.7) as pain target.

Metal-Organic Framework Featuring Cubic Caged Structures for One-Step Ethylene Purification from Ethylene/Ethane Mixtures.

Separation of CH/CH mixtures is of significant importance in the chemical industry but remains a challenge due to the physicochemical similarities of CH and CH. Herein, a metal-organic framework (MOF), [Zn(μ-O)(PCTF)] (Zn-PCTF) (PCTF= 5-trifluoromethyl-1H-pyrazole-4-carboxylic), is provided for the removal of CH from CH/CH mixtures. Zn-PCTF displays a three-dimensional framework featuring one-dimensional pore channels with periodic bottleneck segments.

Advances in the mechanisms and applications of RNA silencing in crop protection.

RNA silencing (or RNA interference, RNAi) is a conserved mechanism for regulating gene expression in eukaryotes, which plays vital roles in plant development and response to biotic and abiotic stresses. The discovery of trans-kingdom RNAi and interspecies RNAi provides a theoretical basis for exploiting RNAi-based crop protection strategies. Here, we summarize the canonical RNAi mechanisms in plants and review representative studies associated with plant-pathogen interactions.

Metal-Organic Frameworks Possessing Suitable Pores for Xe/Kr Separation.

Adsorption separation of the Xe/Kr mixture remains a tough issue since Xe and Kr have an inert nature and similar sizes. Here we present a chlorinated metal-organic framework (MOF) [JXNU-19(Cl)] and its nonchlorinated analogue (JXNU-19) for Xe/Kr separation. The two isostructural MOFs constructed from the heptanuclear cobalt-hydroxyl clusters bridged by organic ligands are three-dimensional structures.

Clinical application of multidisciplinary team- and evidence-based practice project in gynecological patients with perioperative hypothermia.

Background: Perioperative hypothermia (PH) negatively affects the physical and mental health of patients to varying degrees. Currently, there is no effective multidisciplinary team (MDT) intervention for gynecological patients with PH.

Aim: To apply the best evidence on the prevention and management of PH in gynecological patients, improve the quality of perioperative evidence-based care based on treatment by an MDT for gynecological patients and analyze the effect of MDT- and evidence-based practice (EBP) projects on the psychological status and cognitive function of gynecological patients with PH.

Cucurbituril-Shaped Cd(triazolate) Unit-Based Metal-Organic Framework Exhibiting an CH/CO Separation Ability.

Herein, a metal-organic framework (MOF), {[(MeNH)][Cd(HO)][Cd(TrZ)(TPD)(DMF)]} (denoted as JXNU-18, TrZ = triazolate), constructed from the unique cucurbituril-shaped Cd(TrZ) secondary building units bridged by 2,5-thiophenedicarboxylic (TPD) ligands, is presented. The formation of the cucurbituril-shaped Cd(TrZ) unit is unprecedented, demonstrating the geometric compatibility of the organic linkers and the coordination configurations of the cadmium atoms. Each Cd(TrZ) unit is connected to eight neighboring Cd(TrZ) units through 30 TPD linkers, affording the three-dimensional structure of JXNU-18.

Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation.

Astrocytes are intimately linked with brain blood vessels, an essential relationship for neuronal function. However, astroglial factors driving these physical and functional associations during postnatal brain development have yet to be identified. By characterizing structural and transcriptional changes in mouse cortical astrocytes during the first two postnatal weeks, we find that high-mobility group box 1 (Hmgb1), normally upregulated with injury and involved in adult cerebrovascular repair, is highly expressed in astrocytes at birth and then decreases rapidly.

Differential Expression of Transporter Genes in Brain Vessels vs. Peripheral Tissues and Vessels from Human, Mouse and Rat.

Background: ATP-binding cassette (ABC) transporters comprise a superfamily of genes encoding membrane proteins with nucleotide-binding domains (NBD). These transporters, including drug efflux across the blood-brain barrier (BBB), carry a variety of substrates through plasma membranes against substrate gradients, fueled by hydrolyzing ATP. The expression patterns/enrichment of transporter genes in brain microvessels compared to peripheral vessels and tissues are largely uncharacterized.

Boosting the CH/CO Separation Performance of Metal-Organic Frameworks through Fluorine Substitution.

A pair of metal-organic frameworks (MOFs) of JXNU-15 (formulated as [Co(μ-OH)(BTB)(BPY)], BTB = benzene-1,3,5-tribenzoate and BPY = 4,4'-bipyridine) and its fluorinated JXNU-15(F) ([Co(μ-OH)(SFBTB)(BPY)]) based on the fluorous 1,3,5-tri(3,5-bifluoro-4-carboxyphenyl)benzene (SFBTB) ligands were presented. The detailed comparisons of the acetylene/carbon dioxide (CH/CO) separation abilities between the isostructural JXNU-15(F) and JXNU-15 were presented. In comparison with the parent JXNU-15, the higher CH uptake, larger adsorption selectivity of the CH/CO (50/50) mixture, and enhanced CH/CO separation performance endow JXNU-15(F) with highly efficient CH/CO separation performance, which is demonstrated by singe-component gas adsorptions and dynamic gas mixture breakthrough experiments.

Treatment of stellate ganglion block in diseases: Its role and application prospect.

The stellate ganglion (SG), as a type of sympathetic ganglion, consists of the sixth and seventh cervical vertebrae and the first thoracic sympathetic ganglia. SG block (SGB) is a minimally invasive injection that aims to inject low-concentration local anesthetics to induce a broad sympathetic blocking effect near the SG. There have been no changes and progress in the clinical application of SGB since the 1830s due to several potential risks, including hematoma from blood vessel injury, hoarseness from recurrent laryngeal nerve injury, and cardiopulmonary arrest.

Dicopper(II) paddle-wheel metal-organic frameworks for high propyne storage under ambient conditions.

Fluorinated dicopper(II) metal-organic framework JXNU-16F with 1,3,5-tri(3,5-bifluoro-4-carboxyphenyl)benzene ligands and nonfluorinated JXNU-16 exhibit high propyne uptakes of 443 and 496 cm g under ambient conditions, respectively. Their remarkable propyne uptakes result from suitable pore spaces and strong propyne⋯propyne interactions amongst the adsorbed propyne molecules, as revealed by computational simulations.

Enhancement of Propadiene/Propylene Separation Performance of Metal-Organic Frameworks by an Amine-Functionalized Strategy.

Here, a hexanuclear Co(μ-OH) cluster-based metal-organic framework (MOF), [Co(μ-OH)(BTB)(bpy)] (JXNU-15) (bpy = 4,4'-bipyridine), with the 1,3,5-tri(4-carboxyphenyl)benzene (BTB) ligand was synthesized for the challenging propadiene/propylene separation. The combination of a large pore volume and a suitable pore environment boosts the significantly high propadiene (CH) uptake (311 cm g at 298 K and 100 kPa) for JXNU-15. An amine-functionalized MOF of JXNU-15(NH) was further obtained with the 1,3,5-tri(4-carboxyphenyl)benzene analogue of 3,3″-diamino-5'-(3-amino-4-carboxyphenyl)-[1,1':3',1″-terphenyl]-4,4″-dicarboxylic ligand.

Dinuclear Nickel-Oxygen Cluster-Based Metal-Organic Frameworks with Octahedral Cages for Efficient Xe/Kr Separation.

Xe/Kr separation is industrially important but remains a daunting issue in chemical separations. Herein, a fluorinated metal-organic framework (MOF), [Ni(μ-O)(TFBPDC)(tpt)] (named JXNU-13-F), built from 3,3',5,5'-tetrakis(fluoro)biphenyl-4,4'-dicarboxylic (TFBPDC) and 2,4,6-tri(4-pyridinyl)-1,3,5-triazine (tpt) ligands is provided. JXNU-13-F displays a three-dimensional (3D) framework constructed from distorted octahedral cages and an impressive Xe capacity of 144 cm g at 273 K and 1 bar, ranking among top MOFs.

Novel Transcriptional and Translational Biomarkers of Tularemia Vaccine Efficacy in a Mouse Inhalation Model: Proof of Concept.

subspecies ( is extremely virulent for humans when inhaled as a small particle aerosol (<5 µm). Inhalation of ≥20 viable bacteria is sufficient to initiate infection with a mortality rate ≥30%. Consequently, in the past, became a primary candidate for biological weapons development.

[Study of the Relationship between Oral Diseases and Depression Symptoms in Middle-Aged and Older Adult Populations in China-A Retrospective Study Based on CHARLS Data].

Objective: To study the relationship between oral disease and depressive symptoms in middle-aged and older adult populations in China.

Methods: The data from the China Health and Retirement Longitudinal Study (CHARLS) done between 2013 and 2015 were analyzed. A total of 3828 middle-aged and older adults showing no depressive symptoms in an assessment with the 10-item Centre for Epidemiological Studies Depression Scale (CES-D-10) were selected as the subjects of observation, and oral disease was taken as the dependent variable.

Metal-Organic Frameworks Featuring 18-Connected Nonanuclear Rare-Earth Oxygen Clusters and Cavities for Efficient CH/CO Separation.

Two rare-earth (RE) metal-organic frameworks (MOFs) formulated as {(MeNH)[RE(μ-OH)(μ-OH)(DCPB)(HO)]} (RE = Y and Tb; termed JXNU-10) built from a triangular 3,5-di(4'-carboxylphenyl)benzoic acid (DCPB) ligand are presented. JXNU-10 features the rarely observed 18-connected nonanuclear [RE(μ-OH)(μ-OH)] clusters, one-dimensional-nanosized tubular channels, and trigonal-bipyramidal cavities. The presence of the high-nuclear RE-oxo clusters and the robust coordination bonds between the highly charged RE ions and the hard base of the carboxylate/hydroxyl oxygen atoms yielded the water-resistant JXNU-10 materials.

Octanuclear Cobalt(II) Cluster-Based Metal-Organic Framework with Caged Structure Exhibiting the Selective Adsorption of Ethane over Ethylene.

A novel metal-organic framework (MOF) of [Co(OH)(TCA)(HO)] (abbreviation: JXNU-9) based on the unique octanuclear Co(μ-OH) clusters linked by 4,4',4″-nitrilotribenzoate (TCA) ligands featuring small caged structures and one-dimensional channels was prepared and characterized. JXNU-9 shows a high CH uptake capacity of 3.60 mmol g (4.

Trans-kingdom RNAs and their fates in recipient cells: advances, utilization, and perspectives.

The phenomenon and potential mechanisms of trans-kingdom RNA silencing (or RNA interference, RNAi) are among the most exciting topics in science today. Based on trans-kingdom RNAi, host-induced gene silencing (HIGS) has been widely applied to create crops with resistance to various pests and pathogens, overcoming the limitations of resistant cultivars. However, a lack of transformation technology in many crops limits the application of HIGS.

Structural Evolution from Noninterpenetrated to Interpenetrated Thorium-Organic Frameworks Exhibiting High Propyne Storage.

Two thorium-organic frameworks of [ThO(OH)(TFBPDC)(HO)] () and [ThO(OH)(TFBPDC)(HCOO)(HO)] () constructed from the 3,3',5,5'-tetrakis(fluoro)biphenyl-4,4'-dicarboxylate (TFBPDC) ligand were obtained in a reaction. At an early stage of the reaction, the formation of the three-dimensional (3D) framework of was discovered. At a later stage of the reaction, the complete product of was obtained.

[Nutritional Knowledge and Skills of Fast-food Takeout Practitioners in Chengdu City].

Objective To investigate the current status of nutritional knowledge and skills of fast-food takeout practitioners in Chengdu City,so as to provide evidence for nutritional literacy education among takeout practitioners.Methods A questionnaire survey was conducted among 832 employees of fast-food takeout restaurants in Chengdu from April to September in 2019 through a multi-stage random sampling strategy.Results The awareness rate of nutritional knowledge of fast-food takeout practitioners in Chengdu was 77.

Metal-organic frameworks for CH/CO separation.

The separation of C2H2 and CO2 is of industrial significance but is highly challenging due to their close similarities in physical-chemical properties. Metal-organic frameworks (MOFs) with tailored pore structures and tunable pore surfaces for the separation of C2H2/CO2 mixture are described in this perspective article. In terms of adsorption selectivity, MOF is classified into C2H2-selective MOF and CO2-selective MOF, which are discussed separately.

[Young Children's Family Sex Education in Rural Areas of Sichuan Province and Its Influencing Factors].

To understand the family sex education for young children in rural areas of Sichuan province and analyze the influencing factors. A multi-stage random sampling method was used to select 2246 parents of kindergarten children from rural areas in Sichuan province for a questionnaire-based survey.The Chi-square test and Logistic regression model were used for data analysis.

Proton-Conductive Coordination Polymers Based on Diphenylsulfone-3,3'-disulfo-4,4'-dicarboxylate with Well-Defined Hydrogen Bonding Networks.

The diphenylsulfone-3,3'-disulfo-4,4'-dicarboxylic acid (H-DPSDSDC) ligand and its coordination polymers, [KZn(CHSO)(HO)] () and {[Cu(μ-OH)(CHSO)(HO)(DMF)]·3(HO)} () (CHSO = diphenylsulfone-3,3'-disulfo-4,4'-dicarboxylate), were synthesized. The Zn(HO) units in are connected by DPSDSDC ligands to generate a one-dimensional (1D) chain, which is bridged by K-O bonds associated with bridging water molecules and sulfonate groups to yield a two-dimensional (2D) layer. In , the 1D hydroxyl-bridging Cu(II) chains are connected by DPSDSDC ligands to give a 2D layer.

Differential expression of receptors mediating receptor-mediated transcytosis (RMT) in brain microvessels, brain parenchyma and peripheral tissues of the mouse and the human.

Receptor-mediated transcytosis (RMT) is a principal pathway for transport of macromolecules essential for brain function across the blood-brain barrier (BBB). Antibodies or peptide ligands which bind RMT receptors are often co-opted for brain delivery of biotherapeutics. Constitutively recycling transferrin receptor (TfR) is a prototype receptor utilized to shuttle therapeutic cargos across the BBB.