Effectiveness of Total Hip Arthroplasty Combined With Hip Braces for Hip Charcot Arthropathy.

Objective: Charcot arthropathy is characterized by varying degrees of sensory loss and rapidly progressive joint destruction. Historically, limited studies indicated that Charcot arthropathy of the hip joint may be a contraindication for total hip arthroplasty (THA). Yet, some recent studies have shown that good clinical results and acceptable survival rate.

Inhibition of autophagy attenuates cognitive decline and mitochondrial dysfunction in an Alzheimer's disease mouse model with chronic cerebral hypoperfusion.

This study aimed to investigate the impact of chronic cerebral hypoperfusion (CCH) on cognitive function, amyloid-β (Aβ) deposition, cellular autophagy, and mitochondrial dynamics in an Alzheimer's disease (AD) mouse model, and to evaluate the intervention effects of autophagy modulation on these outcomes. Utilizing the APP/PS1 mouse model combined with CCH, we assessed cognitive function, Aβ deposition, and the expression levels of relevant proteins through behavioral tests and immunohistochemical analysis. Our findings revealed pronounced cognitive deficits and increased Aβ deposition in the AD + CCH group mice, along with upregulation of mitochondrial fission proteins (Drp1, Fis1) and downregulation of mitochondrial fusion proteins (Opa1, Mfn1), indicating a shift towards mitochondrial fission and promoting cell apoptosis.

177Lu Radiolabeled Polydopamine Decorated with Fibroblast Activation Protein Inhibitor for Locoregional Treatment of Glioma.

Radionuclide therapy is expected to be a powerful tool for glioma treatment. Here, we introduce a novel nuclear nanomedicine based on polydopamine (PDA), incorporating fibroblast activation protein inhibitor (FAPI) and macrocyclic chelator (DOTA) for specific cancer targeting and 177Lu labeling. The synthesized nanoradiopharmaceutical, 177Lu-DOTA-PEG-PDA-FAPI, exhibits good stability in serum, saline and PBS over 5 days.

Genome-Wide Identification and Expression Analysis of /s in L.

Pepper ( L.) is a popular vegetable in people's daily lives. During pepper growth, calcium (Ca) is an essential macronutrient, and calcium-transporting ATPase (ACA/ECA) is a vital protein for calcium transport.

Isolation and manipulation of meiotic spindles from mouse oocytes reveals migration regulated by pulling force during asymmetric division.

Spindles are essential for accurate chromosome segregation in all eukaryotic cells. This study presents a novel approach for isolating fresh mammalian spindles from mouse oocytes, establishing it as a valuable model system for a wide range of possible studies. Our method enables the investigation of the physical properties and migration force of meiotic spindles in oocytes.

Construction of Pt─O Sites on Pt Nanoclusters in Silicalite-1 Zeolite for Efficient Catalytic Oxidation of Hydrogen Isotope Gases.

The construction, use, and maintenance of tritium-related equipment will inevitably produce tritium-containing radioactive waste gas, and the production of efficient catalysts for tritium removal remains a difficult problem. Herein, silicalite-1 zeolite with entrapped Pt nanoclusters is skillfully post-oxidized at an appropriate temperature, building highly active Pt─O sites on the nanoclusters to achieve efficient oxidation of hydrogen isotopes at low temperatures. The designed Pt─O sites can directly participate in the oxidation reaction of hydrogen isotopes.

A novel protein corona-induced aggregation-ECL strategy based on poly-l-cys/Cu NCs for detecting microplastics in water.

To address the health risks posed by microplastics (MPs), this work developed a poly-l-cysteine (poly-L-cys)-based electrochemiluminescence (ECL) sensor for detecting MPs in water environments. The porous structure of poly-L-cys film can regulate the generation of copper nanoclusters (Cu NCs) in the pores, effectively limiting the migration and aggregation of nanopaticles. In addition, poly-L-cys film also acted as co-reactant promoters, promoting electron transfer and effectively enhancing ECL signal of Cu NCs.

Exosomes derived from umbilical cord mesenchymal stem cells ameliorate ischemic brain injury in mice by regulating AAK1 via miR-664a-5p.

Objective: To identify the molecular targets of mesenchymal stem cell (MSC)-derived exosomes in treating cerebral ischemia and elucidate their therapeutic mechanisms.

Methods: We utilized a mouse model of middle cerebral artery occlusion and treated mice with umbilical cord mesenchymal stem cells derived exosomes. Proteomic analysis identified AAK1(AP2 associated kinase 1) as a key target protein.

Regulation of AUF1 alternative splicing by hnRNPA1 and SRSF2 modulate the sensitivity of ovarian cancer cells to cisplatin.

Purpose: Clarification of cisplatin resistance may provide new targets for therapy in cisplatin resistant ovarian cancer. The current study aims to explore involvement of isoforms of AU-rich element RNA-binding protein 1 (AUF1) in cisplatin resistance in ovarian cancer.

Methods: The cancer stem cell-like features were analyzed using colony formation assay, tumor sphere formation assay and nude mouse xenograft experiments.

Development of Novel Silicon-Based Hydrophobic Tags (SiHyT) for Targeted Proteins Degradation.

Recent advances in targeted protein degradation (TPD) have propelled it to the forefront of small molecular drug discovery. Among these, hydrophobic tagging (HyT) strategies have garnered significant interest. Carbon-based hydrophobic tags have been recognized as effective Hyts for degrading a variety of target proteins.

A diffusion tensor imaging-based multidimensional study of brain structural changes after long-term high-altitude exposure and their relationships with cognitive function.

Background: Brain structure changes after long-term adaptation to the high-altitude environment; however, related studies are few, results are in consistent, and long-term effects on cognitive function and pathophysiological mechanisms are unclear. Therefore, diffusion tensor imaging (DTI) was used to investigate the damage to white matter fiber tracts and correlations between brain structural abnormalities and cognitive function.

Methods: Forty healthy Han people living on the high-altitude and 40 healthy Han people living on the plains were enrolled in this study and underwent magnetic resonance imaging, emotional state assessment, and cognitive function tests.

Gentiopicroside Attenuates Lithium/Pilocarpine-Induced Epilepsy Seizures by Down-Regulating NR2B/CaMKII/CREB and TLR4/NF-κB Signaling Pathways in the Hippocampus of Mice.

Epilepsy is a prevalent and disabling neurological condition characterized by recurrent seizures. Approximately 50% of adults with active epilepsy have at least one comorbidity and they are at a greater risk of premature death than the general population. Gentiopicroside (Gent) is a primary component of Pall.

ZnO NPs Impair the Viability and Function of Porcine Granulosa Cells Through Autophagy Regulated by ROS Production.

The zinc oxide nanoparticles (ZnO NPs) is one of the most extensively utilized metal oxide nanoparticles in biomedicine, human food, cosmetics and livestock farming. However, growing evidence suggests that there is a potential risk for humans and animals because of the accumulation of ZnO NPs in cells, which leads to cell death through several different pathways. Nevertheless, the effects of ZnO NPs on porcine granulosa cells (PGCs) and how ZnO NPs regulate the follicular cells are unknown.

Neoadjuvant PD-(L)1 blockade with or without chemotherapy versus chemotherapy alone in mismatch repair-deficient, potentially resectable stage III-IV gastric cancer patients: a single-center retrospective study.

Background: Currently, PD-(L)1 blockade-based neoadjuvant treatment has shown promising outcomes in patients with potentially resectable gastric cancer. In this real-world study, we aimed to retrospectively observe the efficacy including tumor response and event-free survival (EFS), and safety of PD-(L)1 blockade-based neoadjuvant treatment versus chemotherapy alone in potentially resectable gastric cancer patients with microsatellite instability-high (MSI-H) or mismatch-repair deficient (dMMR) status.

Methods: We retrospectively collected the clinical data of patients with potentially resectable gastric cancer and MSI-H/dMMR status who received neoadjuvant treatment followed by D2 gastrectomy at the Affiliated Hospital of Qingdao University from January 2019 to June 2023.

Animal experimental study of 3D-printed titanium implants based on magnesium-zinc ion surface modification to promote oral soft-tissue closure.

Objective: The study aimed to investigate whether 3D-printed titanium implants modified with magnesium and zinc ion surfaces can promote oral soft-tissue closure.

Method: New Zealand Great White rabbits were selected as experimental animals, and the left and right side mandibular teeth of each animal were randomly divided into an experimental group and control group, each with 18 cases, and the bilateral first premolar teeth were extracted after general anesthesia, and implants were implanted into the magnesium/zinc ionized surface-treated and the surface-untreated groups, respectively.

Results: Under naked-eye observation, the combination of implant material and surrounding soft tissue in the experimental group was significantly better than that in the control group; fluorescence staining showed that the fluorescence density value of the experimental group was significantly higher than that of the control group (p < 0.

Curcumin ameliorates astrocyte inflammation through AXL in cuprizone-induced mice.

Curcumin has gained global attention owning to its anti-inflammatory, antioxidant, anticancer, and antimicrobial activities. Curcumin has recently been shown to have well-documented effects on neuroinflammation in multiple sclerosis (MS). Astrocytes, the most widely distributed glial cells in the brain, have a significant influence on the regulation of neuroinflammation in MS.

SnS QDs@MXene Ohmic Junction-Based Surface Plasmon Coupling ECL Sensor to Detect Saliva Exosome for the Diagnosis of Childhood Asthma.

This study represents a novel surface plasmon coupling electrochemiluminescence (SPC-ECL) method for detecting salivary exosomes and the diagnosis of childhood asthma. First, SnS QDs@MXene Ohmic junctions was developed as efficient ECL emitters. The Ohmic junction provided a low resistance to reduce the contact resistance and improve charge injection efficiency, which enhanced the ECL signal by 2.

Mechanism of effect and therapeutic potential of NLRP3 inflammasome in spinal cord injury.

Spinal cord injury (SCI) is a serious and disabling central nervous system injury that can trigger various neuropathological conditions, resulting in neuronal damage and release of various pro-inflammatory mediators, leading to neurological dysfunction. Currently, surgical decompression, drugs and rehabilitation are primarily used to relieve symptoms and improve endogenous repair mechanisms; however, they cannot directly promote nerve regeneration and functional recovery. SCI can be divided into primary and secondary injuries.

Unraveling Ion Migration Mechanisms under Operating Conditions in Perovskite Solar Cells by Variable-Load Transient Photoelectric Technique.

Ion migration in perovskite solar cells (PSCs) significantly impacts their photoelectric performance and physicochemical stability. Existing research has predominantly focused on inhibiting ion migration through chemical strategies or observing it under open-circuit or short-circuit conditions. This focus has led to a limited theoretical understanding and control of ion migration under practical conditions, constraining advances in long-term stability.

Updated breast cancer costs for women by disease stage and phase of care using population-based databases.

Background: This study assessed health care system costs and resource utilization for adult women with breast cancer in Ontario, Canada. The goal was to update costs by stage, age, and phase of care from a health care system perspective.

Data And Methods: A retrospective analysis was conducted using linked population-based administrative data.