Study on the Effect of Bee Venom and Its Main Component Melittin in Delaying Skin Aging in Mice.

Bee venom (BV) and its main compound melittin (MLT) have antioxidant, anti-inflammatory, and anti-aging activities; however, very little research has been conducted on their effects on skin aging. In this study, a mouse skin aging model induced by D-galactose was constructed via subcutaneous injection into the scruff of the neck, and different doses of BV and MLT were used as interventions. The anti-aging effects and mechanisms of BV and MLT were explored by detecting the skin morphology and structure, and anti-aging-related factors and performing non-targeted metabolomics of mice.

Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes.

Background: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents.

Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB agents.

Results: Using the combined approach, 11 bioactive compounds were isolated and identified, all exhibiting anti- BCG activity.

The synergistic antibacterial effects of allicin nanoemulsion and ε-polylysine against Escherichia coli in both planktonic and biofilm forms.

The synergistic effects of allicin nanoemulsion (AcN) and ε-polylysine (ε-PL) against Escherichia coli were investigated in this study. The combination of AcN and ε-PL synergistically inhibited the planktonic growth of E. coli, with a low fractional inhibitory concentration index of 0.

Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in breast cancer lymphatic node, bone, and brain metastases.

Breast cancer is the most common malignant tumor in the world, and its metastasis is the main cause of death in breast cancer patients. However, the differences between primary breast cancer tissue and lymphatic node, bone, and brain metastases at the single-cell level are not fully understood. We analyzed the microenvironment heterogeneity in samples of primary breast cancer (n = 4), breast cancer lymphatic node metastasis (n = 4), breast cancer brain metastasis (n = 3), and breast cancer bone metastasis (n = 2) using single-cell sequencing data from the GEO database.

Informing Risk Hotspots and Critical Mitigations for Rainstorms Using Machine Learning: Evidence from 268 Chinese Cities.

Climate change is exacerbating rainstorms, increasing the risk of flooding and threatening urban sustainability, which could undermine climate action. Here, we propose a machine learning-based framework to assess heterogeneous risks and identify critical mitigation measures for rainstorms across 268 Chinese cities. Nighttime light serves as a proxy for urban functionality, and meteorological, socio-economic, and infrastructural factors are incorporated to uncover underlying impact mechanisms.

Differences in the Microbial Composition and Function of the Rhizosphere Under Different Cultivation Conditions.

Rhizosphere microorganisms play an important role in the health and development of root systems. Investigating the microbial composition of the rhizosphere is central to understanding the inter-root microbial function of under various cultivation conditions. To complement the metagenomic study of the rhizosphere, here, an amplicon-based metagenomic survey of bacteria and fungi was selected as a practical approach to analyzing the abundance, diversity index, and community structure of rhizosphere bacteria and fungi, as well as to study the effects of different cultivation methods on rhizosphere microbial diversity.

MRI evaluation of neuroprotective effects of Astragaloside Ⅳ on rotenone-induced late-stage Parkinson's disease mice.

Astragaloside Ⅳ (AS-Ⅳ) improved the motor behavior of Parkinson's disease (PD) mouse but the alteration of imaging in the PD mice brain was unclear. PD models were established by unilateral injection of rotenone (ROT) into the substantia nigra pars compacta (SNc) of mice. AS-Ⅳ (20 mg/kg) was intraperitoneally injected once daily for 14 days.

APOM Modulates the Glycolysis Process in Liver Cancer Cells by Controlling the Expression and Activity of HK2 via the Notch Pathway.

The metabolic pathway of aerobic glycolysis in tumor cells has garnered significant attention in tumor research because of its high activation in cancer cells. Previous research conducted by our team has demonstrated that Apolipoprotein M (APOM) exhibits potential as a factor against liver cancer. However, further investigations are needed to elucidate the precise approach and mechanism that are involved in this process.

Regulating Sensing Patterns in Fluorescent Probes for Discriminative Detection of Biothiols.

Discerning and quantifying the critical biothiols cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are vital for understanding their synergistic roles in biological systems. In this study, we synthesized a series of phenylethynylcoumarin fluorescent probes with varied structures to investigate the mechanisms underlying biothiol detection. We found that different substituents (-OCH, -H, -CN) at the -position of the phenylacetylene, combined with an aldehyde group at the 3-position of the coumarin, significantly affected the probes' reactivity and produced distinct response patterns toward biothiols.

The Cross-Sectional Association Between Multimorbidity and Sleep Quality and Duration Among the Elderly Community Dwellers in Northwest China.

Background: Multimorbidity, defined as the coexistence of two or more chronic diseases, is highly prevalent among the elderly population and is associated with adverse outcomes. However, little is known about its relationship with sleep issues, particularly in this demographic. Therefore, this study aimed to investigate its association with sleep quality and duration among the elderly.

Effects of transglutaminase on the gelation properties and digestibility of pea protein isolate with resonance acoustic mixing pretreatment.

Our previous research confirmed that resonance acoustic mixing (RAM) pretreatment effectively improved the emulsification and water retention of commercial pea protein isolate (PPI), but significantly reduced its gel performance. This study aimed to investigate the effect of transglutaminase (TGase, 0.1 %, 0.

How do drivers perceive collision risk? A quantitative exploration in generalized two-dimensional scenarios.

Driving behavior is crucial in shaping traffic dynamics and serves as the foundation for safe and efficient autonomous driving. Despite the widespread interest in driving behavior modeling, existing models often focus on specific behaviors and cannot describe all types of vehicle movements, while vehicle status and driving scenarios are dynamic and infinite. That means comprehending and modeling generalized driving behavior mechanisms is essential.

Decoding the whole-genome sequence of multidrug-resistant strain Hakim RU_BHWS isolated from wastewater in Bangladesh.

This report presents the draft genome sequence of the multidrug-resistant strain Hakim RU_BHWS isolated from wastewater. The genome assembly is 4.6 Mb, with 32.

Safety, immunogenicity and immune-persistence of a lyophilized human rabies vaccine (Vero cells) under Zagreb and Essen regimens: a randomized, open-label, controlled phase III clinical trial in healthy participants aged 10-60 years in China.

Background: Rabies continues to be a significant global public health concern, particularly in the Asia region where it is associated with high mortality rate. The administration of effective vaccination is essential in preventing this potentially fatal viral infection. The objective of this study was to evaluate the immunogenicity and safety of two rabies vaccination schedules: the Zagreb (2-1-1) and Essen (1-1-1-1-1) regimens, in a cohort of healthy Chinese individuals aged 10-60 years.

A nomogram for predicting hemorrhagic transformation in acute ischemic stroke receiving intravenous thrombolysis with extended time window.

A recent randomized controlled clinical trial expanded the time window of intravenous thrombolysis (IVT) in patients with acute ischemic stroke (AIS) beyond 4.5 hours by applying neuroimaging standards, enabling more patients to benefit from IVT. However, hemorrhagic transformation (HT) after IVT is still a major concern.

Peripheral CD4 T cell phenotype and brain microglial activation associated with cognitive heterogeneity in aged rats.

Cognitive decline is a critical hallmark of brain aging. Although aging is a natural process, there is significant heterogeneity in cognition levels among individuals; however, the underlying mechanisms remain uncertain. In our study, we classified aged male Sprague‒Dawley rats into aged cognition-unimpaired (AU) group and aged cognition-impaired (AI) group by using an attentional set-shifting task.

HOXD9/APOC1 axis promotes macrophage M1 polarization to exacerbate diabetic kidney disease progression through activating NF-κB signaling pathway.

Background: Diabetic kidney disease (DKD) is a complication caused by end-stage diabetes mellitus and usually results in glomerular podocyte injury. Exosomes are important for intercellular information exchange. However, the effect of podocyte exosomes on DKD has not been elucidated.

Malassezia gallinae sp. nov., a new basidiomycetous yeast species isolated from skins of chickens.

Species of Malassezia are lipid-dependent yeasts and integral components of the skin microbiome. Most of the currently known species are isolated from mammals. However, the presence and distribution of Malassezia yeasts on the skin of avian species have not been fully understood or elucidated.

Dual-Functional Fluorescent Probe in the Diagnosis of Liver Injury and the Evaluation of Drug Therapy with Double Signal Amplification.

Viscosity and polarity are crucial microenvironmental parameters within cells, intimately linked to the physiological activities of organisms. We constructed and synthesized an innovative dual-functional fluorescent probe, DHBP. In the green channel, the fluorescence signal notably intensifies with decreasing environmental polarity, while in the red channel, fluorescence signal amplification occurs due to the collaborative effects of viscosity and polarity, resulting in more pronounced changes.

Anchoring temporal convolutional networks for epileptic seizure prediction.

. Accurate and timely prediction of epileptic seizures is crucial for empowering patients to mitigate their impact or prevent them altogether. Current studies predominantly focus on short-term seizure predictions, which causes the prediction time to be shorter than the onset of antiepileptic, thus failing to prevent seizures.