Photoprotective carbon redistribution in mixotrophic Haematococcus pluvialis under high light stress.

Mixotrophy of Haematococcus pluvialis is a potential strategy for producing astaxanthin. However, this strategy has not been extensively commercialized because the mixotrophic mechanisms by which H. pluvialis overcomes high light stress are unclear.

A microfluidic-based SERS biosensor with multifunctional nanosurface immobilized nanoparticles for sensitive detection of MicroRNA.

Developing sensitive and miniaturized biosensors for the detection of microRNAs (miRNAs) is highly desirable due to their association with early cancer diagnosis and prognosis. Here, a new microfluidic-based biosensor, combined with multifunctional nanosurface and DSN-assisted target recycle amplification strategy, is designed for the detection of miRNA-21. The design of nanosurface includes gold nanoparticles on porous anodic aluminum oxide (AAO) for surface enhanced Raman scattering (SERS) substrate, Au@Ag core-shell nanoparticles for SERS nanotags and single-stranded DNA (ssDNA) in between for miRNA capture and nanotags immobilization.

Changes in physicochemical and leachate characteristics of microplastics during hydrothermal treatment of sewage sludge.

Sewage sludge is an important source for microplastics (MPs) entering into environment. Hydrothermal treatment has been considered a promising method for reducing MPs in sewage sludge. However, MPs degradation characteristics and mechanism during sludge hydrothermal treatment are not fully understood.

A Landscape Study on COVID-19 Immunity at the Single-Cell Level.

Since 2019, the coronavirus (COVID-19) has outbroken continuously, spreading internationally and threatening the public health. However, it was unknown how the disorder at the single-cell level was associated with the pathogenesis of COVID-19. This study presented the disorders of macrophages, epithelial cells, CD8 T cells, and natural killer (NK) cells at the single-cell level in the courses of COVID-19 and analyzed the immune response to cytokine storm.

Enhancing the Antifouling Ability of a Polyamide Nanofiltration Membrane by Narrowing the Pore Size Distribution via One-Step Multiple Interfacial Polymerization.

Application of nanofiltration membranes in industries still has to contend with membrane fouling that causes a significant loss of separation performance. Herein, an innovative approach to design antifouling membranes with a narrowed pore size distribution by interfacial polymerization (IP) assisted by silane coupling agents is reported. An aqueous solution of piperazine anhydrous (PIP) and γ-(2,3-epoxypropoxy) propytrimethoxysilane (KH560) is employed to perform IP with an organic solution of trimesoyl chloride and tetraethyl orthosilicate (TEOS) on a porous support.

The value of diffusion weighted imaging in predicting the clinical progression of perforator artery cerebral infarction.

Objectives: To investigate the value of diffusion weighted imaging (DWI) in predicting the clinical progression of perforator artery cerebral infarction.

Methods: The magnetic resonance imaging (MRI) data of patients with perforator artery cerebral infarction hospitalized in our hospital from October 2015 to February 2022 were analyzed retrospectively. Then we compared the differences of apparent diffusion coefficient (ADC) value, maximal size, location of cerebral infarction, clinical data and treatment plan between the two groups.

Development and validation of a meta-learning-based multi-modal deep learning algorithm for detection of peritoneal metastasis.

Purpose: The existing medical imaging tools have a detection accuracy of 97% for peritoneal metastasis(PM) bigger than 0.5 cm, but only 29% for that smaller than 0.5 cm, the early detection of PM is still a difficult problem.

Effects of four endophytic bacteria on cadmium speciation and remediation efficiency of Sedum plumbizincicola in farmland soil.

Cadmium (Cd) pollution in farmland soils severely affects agricultural production safety, thereby threatening human health. Sedum plumbizincicola is a Cd and Zn hyperaccumulator commonly used for the phytoremediation of Cd-contaminated soil. This study was aimed to improve the remediation effect of S.

ARNI or ARB Treats Residual Left Ventricular Remodelling after Surgery for Valvular Regurgitation: ReReRe study protocol.

Aims: Patients with persistent or de novo left ventricular (LV) dilation and/or reduced ejection fraction (EF) after correction for primary aortic (AR) or mitral (MR) regurgitation (i.e. residual LV remodelling) have not been well studied with regard to guideline-directed medical therapy after successful aetiology-reversing surgery.

Transcatheter Mitral Valve-in-Valve Implantations Using Inverted J-Valve.

Background: As bioprosthetic valves are being widely used, the incidence of structural valve deterioration increases, as well as the need for reoperation. Transcatheter mitral valve-in-valve implantations are being increasingly adopted as an alternative to redo-surgical mitral replacement for patients with high surgical risks. This study reports a series of transcatheter mitral valve-in-valve implantations using inverted J-valves.

Two Innovative Fumaric Acid Bridging Lanthanide-Encapsulated Hexameric Selenotungstates Containing Mixed Building Units and Electrochemical Performance for Detecting Mycotoxin.

Two particular fumaric acid bridging lanthanide-encapsulated selenotungstates [HN(CH)]Na[Ln(HO)] [WO(CHO) (CHO)][SeWO][B-α-SeWO]·46HO [Ln = Ce (), La ()] were acquired by the deliberately designed step-by-step synthetic strategy, which are composed of four trilacunary Keggin [B-α-SeWO] and two original [SeWO] building units together with one fumaric acid bridging heterometallic [Ln(HO)][WO(CHO) (CHO)] entity. Particularly, this heterometallic cluster contains four fumaric acid ligands, which play two different roles: one works as the pendant decorating the cluster and the other acts as the linker connecting the whole structure. In addition, the hybrid material was produced through the cation exchange of and dimethyl distearylammonium chloride (DDA·Cl) and its beehive-shaped film of was prepared by the breath figure method, which can be further used to establish an electrochemical biosensor for detecting a kind of mycotoxin-ochratoxin A (OX-A).

Oxidative C-O bond cleavage of dihydroxybenzenes and conversion of coordinated cyanide to carbon monoxide using a luminescent Os(VI) cyanonitrido complex.

The photoreactions of a luminescent osmium(VI) nitrido complex, [Os(N)(L)(CN)] (OsN, HL = 2-(2-hydroxy-5-nitrophenyl)benzoxazole), with catechol (HCat) and hydroquinone (HQ) lead to the cleavage of strong C-OH bonds ( 120 kcal mol) of the dihydroxybenzenes with concomitant conversion of the coordinated cyanide to carbon monoxide.

Conformational changes of tyrosinase caused by pentagalloylglucose binding: Implications for inhibitory effect and underlying mechanism.

Tyrosinase is a critical enzyme related to various pigmentation disorders and browning of fruits and vegetables. In this study, a novel inhibitor pentagalloylglucose (PGG) against tyrosinase was prepared from tannic acid with the chemical structure elucidated using HPLC, ESI-MS, H- and C NMR. Its inhibitory effect and the underlying mechanism on tyrosinase were explored by enzyme kinetics, UV-scanning, copper-ion chelation, fluorescence, circular dichroism, fourier transform infrared spectroscopy and molecular docking simulation.

Quantitative analysis of enhanced CT in differentiating well-differentiated pancreatic neuroendocrine tumors and poorly differentiated neuroendocrine carcinomas.

Objective: To identify quantitative CT features for distinguishing well-differentiated pancreatic neuroendocrine tumors (PNETs) from poorly differentiated pancreatic neuroendocrine carcinomas (PNECs).

Materials And Methods: Seventeen patients with PNECs and 131 patients with PNETs confirmed by biopsy or surgery were retrospectively included. General demographic (sex, age) and CT quantitative parameters (arterial/portal absolute enhancement, arterial/portal relative enhancement ratio, arterial/portal enhancement ratio) were collected.

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2.

Soybean cyst nematode (SCN, Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean ( L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean.

Towards Deep Radar Perception for Autonomous Driving: Datasets, Methods, and Challenges.

With recent developments, the performance of automotive radar has improved significantly. The next generation of 4D radar can achieve imaging capability in the form of high-resolution point clouds. In this context, we believe that the era of deep learning for radar perception has arrived.

Rhabdomyolysis happened after the start of dabigatran etexilate treatment: A case report.

There are few reports of rhabdomyolysis caused by anticoagulants, and it is extremely rare for it to be caused by dabigatran etexilate. An 86-year-old female experienced sudden muscle weakness and pain, a significant increase in Creatine kinase, and renal impairment after oral administration of dabigatran etexilate for 3 weeks. The enhanced thigh MRI showed abnormal signal in multiple thigh muscle groups, indicating that the lesions should be considered inflammatory diseases.

Radiologic Analysis of Causes of Early Recurrence After Percutaneous Endoscopic Transforaminal Discectomy.

Study Design: Retrospective study.

Objective: To analyze the causes of and factors influencing early recurrence after TELD performed in the treatment of lumbar disc herniation.

Methods: We included 285 patients with single-segment lumbar disc herniation treated using transforaminal endoscopy lumbar discectomy TELD from January 2017 to December 2019 at the First Affiliated Hospital of the University of South China.

Assessment of the prognostic value of in clear cell renal cell carcinoma: a bioinformatics analysis.

Background: Clear cell renal cell carcinoma (ccRCC) is one of the most common urological malignancies, and once metastasis occurs, it often has a poor prognosis and lacks effective treatment. Therefore, there is an urgent need to screen some new biomarkers and explore their molecular mechanisms to improve the early clinical diagnosis and targeted therapy of ccRCC. (SPARC/osteonectin, CWCV and Kazal-like domains proteoglycan 1) is a conserved multi-domain proteoglycan that plays an important role in the development of multiple cancer types; however, its prognostic value in ccRCC has not been investigated.

Mitochondrial base editor induces substantial nuclear off-target mutations.

DddA-derived cytosine base editors (DdCBEs)-which are fusions of split DddA halves and transcription activator-like effector (TALE) array proteins from bacteria-enable targeted C•G-to-T•A conversions in mitochondrial DNA. However, their genome-wide specificity is poorly understood. Here we show that the mitochondrial base editor induces extensive off-target editing in the nuclear genome.

Dysregulated behaviour of hair follicle stem cells triggers alopecia and provides potential therapeutic targets.

Due to a steady increase in the number of individuals suffering from alopecia, this condition has recently received increasing attention. Alopecia can be caused by various pathological, environmental or psychological factors, eventually resulting in abnormalities in hair follicle (HF) structures or HF regeneration disorders, especially dysregulated hair follicle stem cell (HFSC) behaviour. HFSC behaviour includes activation, proliferation and differentiation.

Wrist-Ankle Acupuncture as Additional Therapy for Postoperative Multimodal Analgesia in Orthopedic Surgery: Systematic Review and Meta-Analysis.

Background: The present meta-analysis analyzed the efficacy and safety of wrist-ankle acupuncture (WAA) as an additional therapy for postoperative multimodal analgesia after orthopedic surgery.

Methods: Electronic databases, including Cochrane Library, PubMed, EMBASE, Web of Science, CNKI, SinoMed, Wanfang, and VIP, were searched to identify randomized controlled trials and cohort studies that reported details of WAA as an additional therapy for postoperative multiple analgesia in orthopedic surgery before October 1, 2021. Analyzed outcomes included time points of the visual analog scale, use of patient-controlled intravenous analgesia (PCIA), and postoperative adverse events.

Influence of Association Network Properties and Ecological Assembly of the Foliar Fugal Community on Crop Quality.

Revealing community assembly and their impacts on ecosystem service is a core issue in microbial ecology. However, what ecological factors play dominant roles in phyllosphere fungal community assembly and how they link to crop quality are largely unknown. Here, we applied internal transcriptional spacer high-throughput sequencing to investigate foliar fungal community assembly across three cultivars of a Solanaceae crop (tobacco) and two planting regions with different climatic conditions.

Chemical reprogramming of human somatic cells to pluripotent stem cells.

Cellular reprogramming can manipulate the identity of cells to generate the desired cell types. The use of cell intrinsic components, including oocyte cytoplasm and transcription factors, can enforce somatic cell reprogramming to pluripotent stem cells. By contrast, chemical stimulation by exposure to small molecules offers an alternative approach that can manipulate cell fate in a simple and highly controllable manner.

NLRP3, NLRC4 and NLRC5 Gene Polymorphisms Associate with Susceptibility of Pulmonary Aspergillosis in Non-Neutropenic Patients.

Background: Non-neutropenic pulmonary aspergillosis is one of the most common and serious fungal infections. Previous studies have shown that single nucleotide polymorphisms (SNPs) of pattern recognition receptors genes are associated with susceptibility to aspergillosis. NOD-like receptors (NLRs) play an important role in the immunological response against fungal infection.