SiCLAT: simultaneous imaging of chromatin loops and active transcription in living cells.

We present SiCLAT, which introduces a dCas9-dCas13d cassette into the mouse genome. This model enables the stable expression of both dCas9 and dCas13 proteins in diverse cell populations, facilitating concurrent labeling of DNA and RNA across various cell types. Using SiCLAT, we accurately labeled chromatin loop anchor interactions and associated gene transcription during myogenic differentiation.

Manipulation of Surface Potential Distribution Enhances Osteogenesis by Promoting Pro-Angiogenic Macrophage Polarization via Activation of the PI3K-Akt Signaling Pathway.

Regulation of the immune response is key to promoting bone regeneration by electroactive biomaterials. However, how electrical signals at the micro- and nanoscale regulate the immune response and subsequent angiogenesis during bone regeneration remains to be elucidated. Here, the distinctly different surface potential distributions on charged poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)) matrix surfaces are established by altering the dimensions of ferroelectric nanofillers from 0D BaTiO nanoparticles (homogeneous surface potential distribution, HOPD) to 1D BaTiO nanofibers (heterogeneous surface potential distribution, HEPD).

Characterization of the key aroma compounds in cigar filler tobacco leaves from different production regions.

Cigar tobacco leaves exhibited distinct regional characteristics, and aroma compounds were the key substances determining the different style features of cigars. However, the differences in aroma characteristics and the mechanisms of key aroma compound synthesis have not been fully elucidated. This study collected filler tobacco leaves (FTLs) from 5 representative domestic and international production regions.

Collaborative Reduction-Induced Nickel-Catalytic Selective C-S Coupling of Aryl Di/Trithiosulfonates with Aryl Halides.

Metal-catalytic conversion of polysulfide reagents is a major challenge in organic synthesis due to its challenging activation modes of multiple S-S bonds. The utilization of aryl di- and trithiosulfonates in nickel-catalyzed reductive coupling with aryl halides has been unexplored. Herein, we unprecedentedly describe PPh and Zn-collaborative reduction-induced nickel-catalytic selective C-S coupling of aryl di/trithiosulfonates with aryl halides to access sulfides over common disulfides or trisulfides.

Comparative Analysis of Human Brain RNA-seq Reveals the Combined Effects of Ferroptosis and Autophagy on Alzheimer's Disease in Multiple Brain Regions.

Ferroptosis and autophagy are closely associated with Alzheimer's disease (AD). Elevated ferric ion levels can induce oxidative stress and chronic inflammatory responses, resulting in brain tissue damage and further neurological cell damage. Autophagy in Alzheimer's has a dual role.

Electric-Field-Induced Switchable Two-Dimensional Altermagnets.

Altermagnetism, as a recently discovered unconventional antiferromagnetism, allows the lifting of spin degeneracy without net magnetization. The spin splitting of the intrinsic altermagnets is protected by the spin space group symmetry and is therefore difficult to control externally. Here, we propose an extrinsic altermagnet as a complement to the intrinsic altermagnet, whose spin splitting is induced by and can be significantly modulated by the electric field.

Epigenetic activation of JAG1 by AID contributes to metastasis of hepatocellular carcinoma.

Metastasis is a major cause of fatality in hepatocellular carcinoma (HCC), although the precise mechanisms driving the metastatic process remain incompletely understood. In this study, we have made several important findings. Firstly, we have discovered that elevated activation-induced cytidine deaminase (AID) expression is positively correlated with Jagged 1 (JAG1) levels in clinically metastatic HCC patients.

Transcriptional responses of three slc39a/zip members (zip4, zip5 and zip9) and their roles in Zn metabolism in grass carp (Ctenopharyngodon idella).

In order to explore the regulatory mechanism of zip4, zip5 and zip9 in zinc metabolism of grass carp (Ctenopharyngodon idella), the effects of zinc (Zn) on the mRNA expression of zip4, zip5 and zip9 were investigated. Compared to the control, the mRNA levels of zip4 and zip9 were significantly reduced under low and high zinc in L8824 cells; the mRNA expression level of zip5 was significantly increased under low and high zinc incubation. Then, their promoter sequences were cloned, which were 2361 bp, 2004 bp and 2186 bp sequences for zip4, zip5 and zip9 promoters, respectively.

monitoring of quorum sensing signalling molecules using a SERS chip with a micro -chamber array.

The monitoring of quorum sensing (QS) signal molecules in bacterial biofilms is crucial for the diagnosis and treatment of bacterial biofilms. In this work, a surface-enhanced Raman scattering (SERS) chip integrated with functional units of bacterial biofilms and SERS detection is proposed for the monitoring of pyocyanine, an important signal molecule and biomarker secreted by . The SERS chip was composed of a top self-assembled nano-silver SERS substrate layer, a PDMS layer containing a micro-chamber array and bottom quartz layer.

Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from for glucose, mannose, and galactose.

This research identified four amino acid residues (Leu174, Asn297, Tyr301, and Gln291) that contribute to substrate recognition by the high-affinity glucose transporter Xltr1p from . Potential hotspots affecting substrate specificity were selected through homology modeling, evolutionary conservation analyses, and substrate-docking modeling of Xltr1p. Variants carrying mutations at these hotspots were subsequently obtained via in silico screening.

Integrated Whole-Life Cycle Accuracy Valvular Heart Disease Epidemiology Cohort Study (iWAVE): protocol for a prospective cohort study.

Introduction: Despite the rapid advancements in cardiovascular surgery in China, the prevalence of valvular heart disease (VHD) continues to rise, particularly among the elderly population. In the resource-constrained western regions, the lack of an integrated care management system significantly contributes to the burden of cardiovascular disease. Consequently, a comprehensive cohort data platform that encompasses the entire lifespan of patients with VHD is essential.

Roles of macrophages in lupus nephritis.

LN is a serious complication of systemic lupus erythematosus (SLE), affecting up to 60% of patients with SLE and may lead to end-stage renal disease (ESRD). Macrophages play multifaceted roles in the pathogenesis of LN, including clearance of immune complexes, antigen presentation, regulation of inflammation, and tissue repair. Macrophages are abundant in the glomeruli and tubulointerstitium of LN patients and are positively correlated with serum creatinine levels and the severity of renal pathology.

BaTiO Doping Enhances Ultrasound-Driven Piezoelectric Bactericidal Effects of Fibrous Poly(L-Lactic Acid) Dressings to Accelerate Septic Wound Healing.

Bacterial invasion in infected skin wounds triggers inflammation and impedes healing. Current therapeutic strategies incorporating drug interventions within wound dressings often result in drug resistance and delayed healing. Here, we developed a comprehensive therapeutic modality integrating piezoelectric fibrous dressing with controlled ultrasound stimulation for efficient healing in an infected wound model.

Association between cardiorenal syndrome and depressive symptoms among the US population: a mediation analysis via lipid indices.

Background: Cardiovascular diseases (CVD), chronic kidney disease (CKD), and lipids are positively correlated with the presence of depressive symptoms. However, investigation into the complex link that exists between cardiorenal syndrome (CRS) and lipid indices and depression remains scarce.

Methods: This study analyzed data from 11, 729 adults in the National Health and Nutritional Examination Surveys from 2005 to 2018.

Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses.

• Five broad-spectrum mAbs identified from COVID-19 convalescents and vaccinees possessed the ability to recognize spike S2 of pan-coronaviruses. • Five mAbs targeted distinct but conserved epitopes on spike S2 of pan-coronaviruses. • Three of these five mAbs were competitively bound to the fusion peptide epitopes of coronavirus spike protein.

A Chinese CADASIL family with a rare heterozygous mutation in exon 2 of NOTCH3: A case report.

Rationale: Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is an inherited cerebrovascular disease caused by the neurogenic locus notch homolog protein 3 (NOTCH3) gene mutation. In recent years, most of the newly reported mutations of CADASIL patients mainly occur in exon 3 to 24, while the cases related to exon 2 mutation are rare, and clinical research data are relatively insufficient. In this study, we have reported a case of a rare heterozygous mutation c.

Zwitterionic Nanochannels in Covalent Organic Framework Membranes for Improved Flux and Antifouling Property.

Zwitterionic membranes demonstrate excellent antifouling property in water purification. The covalent organic frameworks (COFs), due to the ordered channels and abundant organic functional groups, have distinct superiority in constructing zwitterionic surfaces.Here, the zwitterionic COF membrane is prepared with precise framework structures and uniform charge distribution.

Efficient removal of aqueous ciprofloxacin antibiotic by ZnO/CuO-bentonite composites synthesized via carbon-bed pyrolysis of bentonite and metal co-precipitation.

Antibiotics are of emerging concern due to their widespread use, lack of adequate treatment, and for their potential to threaten human health and the environment. Here, a facile fabrication approach for synthesizing ZnO/CuO-bentonite composites was investigated via carbon-bed pyrolysis of bentonite followed by ZnO/CuO co-precipitation. Sorbents were synthesized using a range of bentonite pyrolysis temperatures, metal oxide contents, and ZnO:CuO mass ratios.

Nicotinate-Modified Lanthanide-Substituted Selenotungstate and Its Catalase-like Activity for the Detection of HO and Ascorbic Acid.

A rigid nicotinate-modified lanthanide-substituted selenotungstate [HN(CH)]NaH[LaSeW(HO)(nica)O][SeWO]·32HO (, Hnica = nicotinic acid) was synthesized and consists of two trivacant Keggin [B-α-SeWO] fragments and one unusual [SeWO] fragment bridged by a heterometallic [LaW(HO)(nica)O] cluster. In the heterometallic cluster, two carboxyl O atoms in two nicotinate ligands directly coordinate with two W atoms in a stable C-O-W-O-W-O six-membered ring fashion. According to its catalase-like activity, was utilized to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce blue oxidized TMB (ox-TMB), which can be used to establish a colorimetric sensing method for the detection of ascorbic acid.

TRβ activation confers AT2-to-AT1 cell differentiation and anti-fibrosis during lung repair via KLF2 and CEBPA.

Aberrant repair underlies the pathogenesis of pulmonary fibrosis while effective strategies to convert fibrosis to normal regeneration are scarce. Here, we found that thyroid hormone is decreased in multiple models of lung injury but is essential for lung regeneration. Moreover, thyroid hormone receptor α (TRα) promotes cell proliferation, while TRβ fuels cell maturation in lung regeneration.

Making an inverted Keggin ion lacunary.

The century-old inverted Keggin ion has been revisited in an effort to unleash its potential in the structural engineering and functional development of polyoxomolybdates (POMos). Over the past hundred years, attempts to program the metal-oxo scaffold of inverted Keggins have been conducted continually but without any success. In this work, a structurally inert, inverted Keggin-type POMo could finally be altered by means of a binary heterogroup-templated approach, resulting in the successful isolation of two lacunary species.

Bidirectional Causal Association Between Chronic Obstructive Pulmonary Disease and Cardiovascular Diseases: A Mendelian Randomization Study.

Background: A large number of studies have demonstrated links between chronic obstructive pulmonary disease (COPD) and cardiovascular diseases (CVDs). However, the causal relationship between COPD and CVDs and the reverse causality remains divergent.

Methods: Exposure and outcome data from the largest available genome-wide association studies were extracted for Mendelian randomization (MR) studies.