Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity.

Cancer cell proliferation requires precise control of E2F1 activity; excess activity promotes apoptosis. Here, we developed cell-permeable and bioavailable macrocycles that selectively kill small cell lung cancer (SCLC) cells with inherent high E2F1 activity by blocking RxL-mediated interactions of cyclin A and cyclin B with select substrates. Genome-wide CRISPR/Cas9 knockout and random mutagenesis screens found that cyclin A/B RxL macrocyclic inhibitors (cyclin A/Bi) induced apoptosis paradoxically by cyclin B- and Cdk2-dependent spindle assembly checkpoint activation (SAC).

The impact of antenatal cluster management on maternal delivery and postpartum rehabilitation.

Objective: Pregnancy care can improve maternal pregnancy outcomes. Cluster nursing, an evidence-based, patient-centered model, enhances pregnancy care, can provide patients with high-quality nursing services, has been widely used in clinical practice in recent years. However, most previous studies evaluated cluster nursing program only for a single clinical scenario.

Preparation, Characterization, and Antioxidant Properties of Self-Assembled Nanomicelles of Curcumin-Loaded Amphiphilic Modified Chitosan.

Curcumin (Cur) is a phytochemical with various beneficial properties, including antioxidant, anti-inflammatory, and anticancer activities. However, its hydrophobicity, poor bioavailability, and stability limit its application in many biological approaches. In this study, a novel amphiphilic chitosan wall material was synthesized.

Effects of Aloe Gel on Lactating Women with Nipple Trauma.

To investigate the efficacy of aloe gel in reducing pain and promoting wound healing in postpartum women with nipple trauma. There were 80 postpartum women who took part in this study having developed nipple trauma during breastfeeding in the obstetrics department of a tertiary grade A hospital in Suzhou from January to December 2021. Postpartum women with nipple trauma whose hospital bed numbers ranged between 15 and 33 were included in the test group, whereas those whose hospital bed numbers ranged between 35 and 53 were included in the control group.

TET3-mediated DNA demethylation modification activates SHP2 expression to promote endometrial cancer progression through the EGFR/ERK pathway.

Objective: Src homology phosphotyrosin phosphatase 2 (SHP2) has been implicated in the progression of several cancer types. However, its function in endometrial cancer (EC) remains unclear. Here, we report that the ten-eleven translocation 3 (TET3)-mediated DNA demethylation modification is responsible for the oncogenic role of SHP2 in EC and explore the detailed mechanism.

Advance nitrogen removal from anaerobic sludge digestion liquor using partial nitrification and denitrification coupled with simultaneous partial nitrification, anammox, and denitrification process.

A novel two-stage continuous-flow partial nitrification and denitrification coupled with simultaneous partial nitrification, anammox, and denitrification (PND-SNAD) process was developed to treat anaerobic sludge digestion liquor. During the stable phase, the total nitrogen and chemical oxygen demand (COD) removal efficiencies were 93 ± 3 % and 59 ± 7 %, respectively. Free ammonia suppression (26.

Self-Assembled Amphiphilic Chitosan Nanomicelles: Synthesis, Characterization and Antibacterial Activity.

Although amphiphilic chitosan has been widely studied as a drug carrier for drug delivery, fewer studies have been conducted on the antimicrobial activity of amphiphilic chitosan. In this study, we successfully synthesized deoxycholic acid-modified chitosan (CS-DA) by grafting deoxycholic acid (DA) onto chitosan C-NH, followed by grafting succinic anhydride, to prepare a novel amphiphilic chitosan (CS-DA-SA). The substitution degree was 23.

The role of segmental bioelectrical impedance technique in the assessment of intraperitoneal ultrafiltration volume with peritoneal dialysis patients.

Objective: To investigate the role of segmental bioelectrical impedance technique (SBIA) in the assessment of intraperitoneal ultrafiltration volume with peritoneal dialysis patients.

Method: We selected the patients at the Department of Nephrology of the First Affiliated Hospital of Zhengzhou University and measured the segmental bioelectrical impedance by a German Fresenius body composition analyzer (the Fresenius whole body composition measurement (BCM) machine was used as a segmental machine in this study). An alternating current (5 kHz, 0.

The potential role of GSK-3β signaling pathway for amelioration actions of ketamine on the PTSD rodent model.

Rationale: Post-traumatic stress disorder (PTSD) is a complex, chronic psychiatric disorder typically triggered by life-threatening events and, as yet, lacks a specialized pharmacological treatment. The potential therapeutic role of ketamine, an N-methyl-D-aspartate receptor antagonist, in mitigating PTSD has been the subject of investigation.

Objective: The aim of this study was to elucidate alterations in the glycogen synthase kinase-3β (GSK-3β) signaling pathway in response to ketamine intervention, using the single prolonged stress (SPS) model of PTSD at a molecular level.

Hyperoxidation active species produced by seawater electro membrane reactor assisted electrolytic cell system for simultaneous decyanation and carbon removal.

Herein, a novel triple-layered heterojunction catalytic cathode membrane (PVDF/rGO/TFe/MnO, TMOHccm) was reported and applied in seawater electro membrane reactor assisted electrolytic cell system (SEMR-EC), achieving increased properties for cyanide wastewater treatment. Hydrophilic TMOHccm exhibits higher electrochemical activity (q* 1.11 C cm, q* 0.

Critical role of Photo-electrode with Ce-g-CN in multi-stage microbial fuel cells cascade reactor treating diluted hyper-saline industrial wastewater rich in amines.

Treatment of chemical industrial wastewater often faces problems of large volume occupation, high cost, and long processing time. In this study, low-content Ce-modified g-CN was prepared and used as a catalyst on stainless steel mesh photo-cathode in constructing a multi-stage cascade microbial fuel cell system to reduce treatment costs in an energy-saving way. The large specific surface area (332.

TSPTFBS 2.0: trans-species prediction of transcription factor binding sites and identification of their core motifs in plants.

Introduction: An emerging approach using promoter tiling deletion via genome editing is beginning to become popular in plants. Identifying the precise positions of core motifs within plant gene promoter is of great demand but they are still largely unknown. We previously developed TSPTFBS of 265 transcription factor binding sites (TFBSs) prediction models, which now cannot meet the above demand of identifying the core motif.

Neuroplastin 65 deficiency reduces amyloid plaque formation and cognitive deficits in an Alzheimer's disease mouse model.

Introduction: Alzheimer's disease (AD) is characterized by increasing cognitive dysfunction, progressive cerebral amyloid beta (Aβ) deposition, and neurofibrillary tangle aggregation. However, the molecular mechanisms of AD pathologies have not been completely understood. As synaptic glycoprotein neuroplastin 65 (NP65) is related with synaptic plasticity and complex molecular events underlying learning and memory, we hypothesized that NP65 would be involved in cognitive dysfunction and Aβ plaque formation of AD.

Inhibiting 5-hydroxytryptamine receptor 3 alleviates pathological changes of a mouse model of Alzheimer's disease.

Extracellular amyloid beta (Aβ) plaques are main pathological feature of Alzheimer's disease. However, the specific type of neurons that produce Aβ peptides in the initial stage of Alzheimer's disease are unknown. In this study, we found that 5-hydroxytryptamin receptor 3A subunit (HTR3A) was highly expressed in the brain tissue of transgenic amyloid precursor protein and presenilin-1 mice (an Alzheimer's disease model) and patients with Alzheimer's disease.

ncRNA-mediated low expression of P2RY14 correlates with poor prognosis and tumor immune infiltration in ovarian carcinoma.

Background: Ovarian cancer (OV) has been puzzling clinicians because of its poor prognosis. More and more evidence show that the G protein coupled receptor P2RY14 plays a key role in the initiation and progression of various types of human cancer. The purpose of our study is to explore the correlation between P2RY14 and the prognosis of ovarian cancer patients and the relevant mechanism.

Systemic investigation of inetetamab in combination with small molecules to treat HER2-overexpressing breast and gastric cancers.

Most patients with metastatic breast cancer or gastric cancer who are treated with trastuzumab, an anti-HER2 monoclonal antibody, become refractory to the drug within a year after the initiation of treatment. Although the combination of trastuzumab with pertuzumab produced synergetic effects in the treatment of HER2-overexpressing cancers, not all patients with HER2 overexpression benefited from the trastuzumab plus pertuzumab combination. To improve the clinical benefits of trastuzumab, we systemically investigated the combination of inetetamab (Cipterbin), an analog of trastuzumab, with a variety of small molecules, including tyrosine kinase inhibitors (TKIs) and chemotherapeutic agents .

Interspecific competition between the bloom-causing dinoflagellates Hetrocapsa bohaiensis and the local species Chlorella pyrenoidosa.

Harmful algal blooms caused by Heterocapsa bohaiensis have broken out in aquaculture areas near Liaodong Bay, China, since 2012, resulting in mass mortality of Eriocheir sinensis larvae and substantial economic loss. Chlorella pyrenoidosa is a local phytoplankton species that is found in aquaculture ponds. However, the reason why H.

Removal of Pharmaceuticals and Personal Care Products (PPCPs) by Free Radicals in Advanced Oxidation Processes.

As emerging pollutants, pharmaceutical and personal care products (PPCPs) have received extensive attention due to their high detection frequency (with concentrations ranging from ng/L to μg/L) and potential risk to aqueous environments and human health. Advanced oxidation processes (AOPs) are effective techniques for the removal of PPCPs from water environments. In AOPs, different types of free radicals (HO·, SO·, O·, etc.

The downregulation of LINC00273 inhibits the proliferation, invasion, and migration of ovarian cancer cells and .

Background: This study sought to analyze long non-coding ribonucleic acid (lncRNA) LINC00273 expression in ovarian cancer tissues, and to preliminarily explore its effect on the growth and invasion of ovarian cancer cells and its influencing mechanism.

Methods: Quantitative real-time polymerase chain reaction was performed to detect the LINC00273 expression levels of cancerous ovarian tissues and their related cell lines. The ovarian cancer cells with the highest expression of LINC00273 were transfected with a knockdown lentiviral vector targeting the LINC00273 sequence and a negative control plasmid.

The relationships among gut microbiota, hypoxia-inducible factor and anaemia with chronic kidney disease.

Gut microbiota plays a vital role in human intestinal homeostasis, correlating strongly with the progression of numerous diseases. Recent researches provide powerful evidence that the connections exist between gut microbiota and renal anaemia. Gut microbiota may have an impact on renal anaemia by regulating the hypoxia-inducible factor (HIF) signalling, iron metabolism and inflammatory state.

Enhanced removal of tetracycline from synthetic wastewater using an optimal ratio of co-culture of Desmodesmus sp. and Klebsiella pneumoniae.

A sustainable approach of Desmodesmus sp. GIEC-179: Klebsiella pneumoniae (DUT-XJR-t-1.2) co-culture ratios were optimized to remove tetracycline (TET) from synthetic wastewater.

Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer.

Ovarian cancer (OC) is a lethal disease occurring in women worldwide. Due to the lack of obvious clinical symptoms and sensitivity biomarkers, OC patients are often diagnosed in advanced stages and suffer a poor prognosis. Circulating tumor cells (CTCs), released from tumor sites into the peripheral blood, have been recognized as promising biomarkers in cancer prognosis, treatment monitoring, and metastasis diagnosis.