Preclinical validation and kinetic modelling of the SV2A PET ligand [F]UCB-J in mice.

Synaptic vesicle protein 2A (SV2A) is ubiquitously expressed in presynaptic terminals where it functions as a neurotransmission regulator protein. Synaptopathy has been reported during healthy ageing and in a variety of neurodegenerative diseases. Positron emission tomography (PET) imaging of SV2A can be used to evaluate synaptic density.

S1 Vertebral Bone Quality Score Independently Predicts Proximal Junctional Kyphosis After Posterior Hemivertebra Resection With Transpedicular Instrumentation.

Objective: Proximal junctional kyphosis (PJK) is a prevalent postoperative complication after posterior hemivertebra (HV) resection. Previous research has demonstrated a strong correlation between the development of PJK and reduced bone mineral density. The vertebral bone quality (VBQ) score, derived from magnetic resonance imaging, is considered a predictive and evaluative tool for bone mineral density.

[Analysis of Carbon Emission Reduction Potential and Discussion on the Green Development Path in Gansu-Qinghai Regions].

The energy resources are rich, and the ecological environment is fragile in Gansu-Qinghai regions, which are facing problems in the coordinated development of green as well as low carbon transformation and high-quality economy. Based on the reality of Gansu-Qinghai regions, this study deeply analyzed the characteristics of regional carbon emissions； constructed the system dynamics model between carbon emissions and population, economy, energy, and policy； clarified the relationship between them； and probed into the future green development path. The results showed that： ① In recent years, the total and per capita carbon emissions in Gansu-Qinghai regions have been on the rise.

Diet Quality Scores and Central Precocious Puberty Risk in Chinese Girls: A Case-Control Study.

Objective: Diet is an important factor influencing central precocious puberty (CPP). This study aimed to investigate the relationship among diet quality, pro-inflammatory diets, and CPP in Chinese girls.

Design, Patients And Measurements: This case-control study enroled 112 Chinese girls with CPP and 131 healthy controls.

An enhancer-promoter-transcription factor module orchestrates plant immune homeostasis by constraining camalexin biosynthesis.

Effective plant defense against pathogens relies on highly coordinated regulation of immune gene expression. Enhancers, as cis-regulatory elements, are indispensable determinants of dynamic gene regulation, but the molecular functions in plant immunity are not well understood. In this study, we identified a novel enhancer, CORE PATTERN-INDUCED ENHANCER 35 (CPIE35), which is rapidly activated upon pathogenic elicitation and negatively regulates anti-fungal resistance through modulating WRKY15 expression.

Machine learning based on multiplatform tests assists in subtype classification of mature B-cell neoplasms.

Mature B-cell neoplasms (MBNs) are clonal proliferative diseases encompassing over 40 subtypes. The WHO classification (morphology, immunology, cytogenetics and molecular biology) provides comprehensive diagnostic understandings. However, MBN subtyping relies heavily on the expertise of clinicians and pathologists, and differences in clinical experience can lead to variations in subtyping efficiency and consistency.

Dissection of major QTLs and candidate genes for seedling stage salt/drought tolerance in tomato.

Background: As two of the most impactful abiotic stresses, salt and drought strongly affect tomato growth and development, especially at the seedling stage. However, dissection of the genetic basis underlying salt/drought tolerance at seedling stage in tomato remains limited in scope.

Results: Here, we reported an analysis of major quantitative trait locus (QTL) and potential causal genetic variations in seedling stage salt/drought tolerance in recombinant inbred lines (n = 201) of S.

Integrated Whole-Life Cycle Accuracy Valvular Heart Disease Epidemiology Cohort Study (iWAVE): protocol for a prospective cohort study.

Introduction: Despite the rapid advancements in cardiovascular surgery in China, the prevalence of valvular heart disease (VHD) continues to rise, particularly among the elderly population. In the resource-constrained western regions, the lack of an integrated care management system significantly contributes to the burden of cardiovascular disease. Consequently, a comprehensive cohort data platform that encompasses the entire lifespan of patients with VHD is essential.

Colchicine inhibits monosodium urate crystal-mediated inflammation by influencing F-actin formation.

Objectives: To understand the mechanism by which colchicine inhibits the inflammatory properties of monosodium urate (MSU) crystal deposits and tophi.

Methods: We investigated the effects of colchicine on the inflammatory properties of monosodium urate (MSU) crystal deposits in several models: (i) In vitro tophus formation by MSU and neutrophils; (ii) MSU-induced peritonitis model; (iii) Alpha-1-antitrypsin-induced peritoneal MSU flare model; (iv) MSU-induced arthritis model. We measured neutrophil numbers, NET formation, IL-1β production and F-actin generation by MSU crystals.

Efficient VOCs photodegradation through local ion substitution in TiO-C composites derived from MIL-125(Ti).

Volatile organic compounds (VOCs) are the main cause of environmental pollution and numerous respiratory diseases. Most VOCs molecules can be entirely converted into HO and CO by photocatalytic technology without the demand for additional oxidants, making it an ideal choice for air purification. However, for conventional photocatalysts, a smaller specific surface area is not conducive to enriching VOCs molecules on the surface of catalyst.

V-domain immunoglobulin suppressor of T-cell activation and programmed death receptor 1 dual checkpoint blockade enhances antitumour immunity and survival in glioblastoma.

Background And Purpose: The current therapy cannot meet the needs of glioblastoma (GBM). V-domain immunoglobulin suppressor of T-cell activation (VISTA) is significantly up-regulated in GBM patients; however, its therapeutic potential in GBM is still unclear.

Experimental Approach: Flow cytometry was used to detect the expression of VISTA and the co-expression pattern of VISTA and programmed death receptor 1 (PD-1) on brain infiltrating lymphocytes of GBM mice.

Correction: An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system.

[This corrects the article DOI: 10.1371/journal.pone.

Research on the relationship between virtual social interaction and the degree of loneliness based on algorithm matching technologies: A quantitative analysis on the SOUL APP-A virtual social software for strangers.

This study examines the relationship between virtual social interaction and people's social behaviors and psychology using algorithm matching technologies and questionnaire surveys. The focus is on interpersonal communication on virtual social platforms. The findings indicate that engaging in virtual social networking is often accompanied by a high level of loneliness.

Juvenile toxicity of atropine sulfate eye drops in young rats.

Objectives: This study was to investigate the effects of atropine sulphate eye drops (ASED)on the development of partial systems in young rats and their toxic reactions following repeated eye-drop administration over a period of 40 days.

Methods: SD rats of 20 days old were randomly assigned to control group, 0.01, 0.

The Mitochondrial Fusion Promoter M1 Mitigates Cigarette Smoke-Induced Airway Inflammation and Oxidative Stress via the PI3K-AKT Signaling Pathway.

Purpose: This study investigated the efficacy and underlying mechanism of the mitochondrial fusion promoter M1 in mitigating cigarette smoking (CS)-induced airway inflammation and oxidative stress both in vitro and in vivo models.

Methods: Cigarette smoke extract (CSE)-treated airway epithelial cells (BEAS-2B) and CS-exposed mice were pretreated with M1, followed by the measurement of proinflammatory cytokines, oxidative stress, mitochondrial fusion proteins (MFN2 and OPA1) and fission proteins (DRP1 and MFF). Molecular pathways were elucidated through transcriptomic analysis and Western blotting.

A Low-Cost Microfluidic Device For the On-Line Counting of Microparticle/Bacteria.

On-line counting of the microparticle/bacteria in the liquid medium has great potential in the food safety and biomedical fields. A new low-cost microfluidic device is proposed for the on-line counting of the microparticles/bacteria in the liquid medium. The gradually contracted microchannel and the viscoelastic fluid are combined to achieve the efficient elastic focusing of the particle/bacteria, which significantly improves the counting accuracy by aligning all particles/bacteria in a single position at the center of the microchannel.

Construction of rhodamine-based fluorescent sensor for fast, on-site quantitative detection of hazardous salicylic acid in practical sample analysis.

Salicylic acid (SA) is widely used in food storage, preservatives, additives, healthcare, and the pharmaceutical industry. However, various poisoning symptoms are frequently reported upon ingestion of a large amount of SA. Therefore, discovering new tools for sensing SA with fast, simple, and portable performance is imperative.

Inhibition of EGFR Pathway Suppresses M1 Macrophage Polarization and Osteoclastogenesis, Mitigating Titanium Particle-Induced Bone Resorption.

Purpose: The polarization of macrophages towards the pro-inflammatory M1 phenotype and osteoclast overactivation play a significant role in the pathogenesis of aseptic loosening of orthopedic implants. This study sought to examine the expression and activation of macrophages and osteoclasts in implant biopsies with respect to epidermal growth factor receptor (EGFR) signaling and to assess the potential of EGFR inhibition in mitigating titanium particle-induced bone resorption in a cranial resorption murine model.

Methods: Bone marrow-derived macrophages (BMDMs) were stimulated with Tumor Necrosis Factor-alpha (TNF-α) and Interferon-gamma (IFN-γ) initially.

Ionizable cationic lipid nanoparticles loaded with miRNA-125b/BLZ945 for pancreatic cancer treatment.

In prior research, both miRNA-125b and BLZ945 have shown potential in effectively inhibiting M2 macrophage polarization and producing antitumor effects. Nevertheless, their physicochemical characteristics present significant challenges for efficient in vivo delivery. Ionizable cationic lipid nanoparticles (LNPs), recognized for their superior biocompatibility and drug-loading capacity, serve as a novel carrier for nucleic acid-based therapeutics.

Effect of emodin on acute lung injury: a meta-analysis of preclinical trials.

Background: Emodin has protective effects on acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). This meta-analysis intended to illustrate the efficacy of emodin on ALI/ARDS animal models.

Methods: Relevant preclinical studies were searched on PubMed, EMBASE, and Web of Science.

TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A.

Background: Tongue squamous cell carcinoma (TSCC) is one of the most common malignant tumors with high mortality and poor prognosis. Its incidence rate is increasing gradually. Tumor necrosis factor receptor-associated factor interacting protein (TRAIP), as a factor related to several tumors, reveals that its gene expression is different between normal tissue and primary tumor of head and neck squamous cell carcinoma using bioinformatics analysis.