Gut microbiota alterations in golden snub-nosed monkeys during food shortage and parturition-nursing periods.

Adopting unique survival strategies during spring food shortages and simultaneous parturition and nursing is crucial for golden snub-nosed monkeys. Social behaviors, such as altruism within one-male units (OMUs), are decisive for family health, but the role of microbiota in regulating these behaviors remains unknown. We conducted the gut microbiota from members of 10 OMUs using 16S RNA sequencing technology.

4D printing polymeric biomaterials for adaptive tissue regeneration.

4D printing polymeric biomaterials can change their morphology or performance in response to stimuli from the external environment, compensating for the shortcomings of traditional 3D-printed static structures. This paper provides a systematic overview of 4D printing polymeric biomaterials for tissue regeneration and provides an in-depth discussion of the principles of these materials, including various smart properties, unique deformation mechanisms under stimulation conditions, and so on. A series of typical polymeric biomaterials and their composites are introduced from structural design and preparation methods, and their applications in tissue regeneration are discussed.

Adherence to rehabilitation exercise and influencing factors among people with acute stroke: a cross-sectional study.

Background: Post-stroke rehabilitation exercise is recognized as the most effective and preferred intervention to reduce disability rates in patients. However, adherence to exercise is low among stroke patients. Previous research has mainly focused on the negative psychological factors influencing adherence, while the positive psychological aspects remain underexplored.

EGFR-mediated crosstalk between vascular endothelial cells and hepatocytes promotes Piezo1-dependent liver regeneration.

Hepatocyte proliferation is essential for recovering liver function after injury. In liver surgery, the mechanical stimulation induced by hemodynamic changes triggers vascular endothelial cells (VECs) to secrete large amounts of cytokines that enhance hepatocyte proliferation and play a pivotal role in liver regeneration (LR). Piezo1, a critical mechanosensory ion channel, can detect and convert mechanical forces into chemical signals, importing external stimuli into cells and triggering downstream biological effects.

Examining the Validity of An Endoscopist-patient Co-participative Virtual Reality Method (EPC-VR) in Pain Relief during Colonoscopy.

To relieve perceived pain in patients undergoing colonoscopy, we developed an endoscopist-patient co-participative VR tool (EPC-VR) based on A Neurocognitive Model of Attention to Pain. It allows the patient to play a VR game actively and supports the endoscopist in triggering a distraction mechanism to divert the patient's attention away from the medical procedure. We performed a comparative clinical study with 40 patients.

Nonclinical safety and biodistribution evaluation of HC009 mRNA vaccine against COVID-19 in rat.

mRNA-based technology has been evaluated in a variety of clinical trials for the rapid control and prevention of emergencies and therapeutic indications. HC009 is a mRNA-based vaccine encoding the full-length of the SARS-CoV-2 spike protein and delivered by the QTsome delivery platform. Here, the immunogenicity, toxicity, and biodistribution of HC009 were investigated in rats.

Spectrum-effect relationship of the cardiovascular-protective effect of with Chrysanthemi Flos by UPLC-MS/MS and component knock-out method.

Chrysanthemi Flos (CF), as one of the important 'dual-use' plants, possesses great pharmacological research and development potential. This work aimed to find the pharmacodynamic material basis of CF in cardiovascular-protection by spectrum-effect relationship and component knock-out method. The fingerprint was established by ultra-high performance liquid chromatography and 25 peaks were picked out as common peaks.

Advances in risk prediction models for cancer-related cognitive impairment.

Cancer-related cognitive impairment (CRCI) has emerged as a significant long-term complication in cancer survivors, particularly those undergoing chemotherapy, radiotherapy, or targeted therapies. Despite advances in treatment, CRCI affects patients' quality of life, impacting their daily functioning, work capacity, and psychological well-being. In recent years, research has focused on identifying predictive factors for CRCI and developing risk prediction models to facilitate early intervention.

Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations.

Decellularized extracellular matrix (dECM) offers a three-dimensional, non-immunogenic scaffold, enriched with bioactive components, making it a suitable candidate for tissue regeneration. Although dECM-based scaffolds have been successfully implemented in preclinical and clinical settings within tissue engineering and regenerative medicine, the mechanisms of tissue remodeling and functional restoration are not fully understood. This review critically assesses the state-of-the-art in dECM scaffolds, including decellularization techniques for various tissues, quality control and cross-linking.

Enhancing Recognition of Stereotyped Movements in ASD Children Through Action Pattern Mining and Multi-Channel Fusion.

Stereotyped movements play a crucial role in diagnosing Autism Spectrum Disorder (ASD). However, recognizing them poses challenges, due to limited data availability and the movements' specificity and varying duration. To support in-depth analysis of ASD children's movements, we constructed the ACSA653 dataset, comprising 653 videos across six classes of stereotyped movements.

A pathogenic NR2F1 gene variant disrupts transcriptional activity and causes severe neurodevelopmental delay in Bosch-Boonstra-Schaaf syndrome.

Introduction: Nuclear receptor subfamily 2, group F, member 1 (NR2F1) gene variations are associated with Bosch-Boonstra-Schaaf optic atrophy syndrome. The NR2F1 genotype correlates with its phenotype; variants within the DNA-binding domain may cause severe psychomotor developmental disorders. However, the mechanisms underlying these phenotypes remain unclear.

Antibiotics in ambient fine particulate matter from two metropolitan cities in China: Characterization, source apportionment, and health risk assessment.

Excessive production and widespread application of antibiotic has led to residues in environmental matrices worldwide. There is limited knowledge of the concentrations of antibiotics bound to ambient fine particulate matter (PM) and their health risks. We investigated the occurrence, sources, environmental driving factors, and health risks of antibiotics in PM samples collected from Beijing and Shijiazhuang, China, during periods of high air pollution.

Aptamer-based and highly sensitive electrochemical label-free gliotoxin biosensor via a dual recycling signal amplification cascade strategy.

Gliotoxin (GT), a mycotoxin produced by Aspergillus fumigatus, exerts immunosuppressive and pro-apoptotic effects on mammalian cells, posing severe health threat upon human. Ultrasensitively and selectively detecting GT is therefore of great significance. In this work, on the basis of a new GT-specific aptamer, we describe construction of electrochemical label-free biosensor for GT with high sensitivity via exonuclease III (Exo III)-aided dual recycling signal amplification strategy.

Naringin Suppresses CoCl-Induced Ferroptosis in ARPE-19 Cells.

Hypoxic damage to retinal pigment epithelial (RPE) cells and subsequent neovascularization are key factors in the pathogenesis of branch retinal vein occlusion (BRVO). Naringin (NG), a naturally occurring flavanone glycoside, has demonstrated significant antioxidant and anti-neovascular activities. However, the regulatory effects and mechanisms of NG on ferroptosis in BRVO are yet to be explored.

Multiscale Dissection of Spatial Heterogeneity by Integrating Multi-Slice Spatial and Single-Cell Transcriptomics.

The spatial structure of cells is highly organized at multiscale levels from global spatial domains to local cell type heterogeneity. Existing methods for analyzing spatially resolved transcriptomics (SRT) are separately designed for either domain alignment across multiple slices or deconvoluting cell type compositions within a single slice. To this end, a novel deep learning method, SMILE, is proposed which combines graph contrastive autoencoder and multilayer perceptron with local constraints to learn multiscale and informative spot representations.

Preparation and Modification of Silicalite-2 Membranes.

Silicalite-2 membranes were successfully prepared on tubular α-AlO supports by secondary hydrothermal synthesis, and the pervaporation performance of the membrane was evaluated by separation of a 5 wt% ethanol/HO mixture at 60 °C. The effects of templating agent content, water-silicon ratio and crystallization time on the separation performance of Silicalite-2 membranes were investigated. When the TBAOH/SiO and HO/SiO molar ratios of the precursor synthesis solution were 0.

Development of a Cascade-Targeting Oral Vaccine via Glycoprotein 2 on Intestinal Microfold Cells for Cancer Immunotherapy.

Oral cancer vaccines are convenient and safe, with the presence of gut-associated lymphoid tissue (GALT) involved intestinal Peyer's patch (PPs) containing microfold (M) cells and housing abundant underneath dendritic cells (DCs). Here, we found that the endocytic receptors glycoprotein 2 (GP2) and dectin-1 are respectively expressed on M cells and DCs with high specificity. Then, we discovered a gastrointestinal hydrolysis-resistant D-peptide GPBP-2(1-8) targeting GP2 by phage display screening and optimization.

Hypoxia-Induced O-GlcNAcylation of GATA3 Leads to Excessive Testosterone Production in Preeclamptic Placentas.

The maintenance of endocrine homeostasis in the placenta is crucial for ensuring successful pregnancy. An abnormally elevated production of placental testosterone (T) has been documented in patients with early-onset preeclamptic (E-PE). However, the underlying mechanisms remain unclear.

Assessment of Knowledge and Training Needs for the Clinical Management of Warfarin Anticoagulation Among Physicians in China.

Objective: To assess the knowledge level of physicians on anticoagulation management and identify their training needs to ensure the safety and efficacy of warfarin therapy among patients.

Methods: This project adopted convenient sampling and collected survey questionnaires online to investigate physicians' management level in aspects such as basic anticoagulation knowledge, routine adjustment of warfarin dosage, and management of warfarin complications and special situations after mechanical valve replacement.

Results: Among the 232 participants in this survey, 158 were male (68.

GreenMedChem-inspired light-air mediated C(sp)-H bond oxidation: A new tool for isoquinolone synthesis.

Isoquinolone represents a significant and promising molecular scaffold for drug development. As green chemistry advances, light-mediated C(sp)-H bond oxidation has emerged as a pivotal tool for medicinal chemists in constructing isoquinolone framework and enabling late-stage functionalization. However, existing methods often rely on metal catalysts, hazardous peroxides, dye sensitizers, or high-temperature atmosphere, which pose environmental concerns.

Highly sensitive and aptamer-based electrochemical fipronil biosensor based on primer exchange reaction and catalytic strand displacement dual recycling amplifications.

Background: The indiscriminate use of pesticides in agriculture has caused substantial harm to both the environment and human health. Therefore, it is crucial to develop methods for evaluating trace amounts of pesticide residues in food products. This study presents a highly sensitive electrochemical aptasensor to detect fipronil utilizing a new primer exchange reaction (PER)/catalytic strand displacement reaction (CSDR)-integrated dual signal amplification strategy.

Advancements in cellular immunotherapy: overcoming resistance in lung and colorectal cancer.

Immunotherapy has revolutionized cancer treatment, offering hope for patients with otherwise treatment-resistant tumors. Among the most promising approaches are cellular therapies, particularly chimeric antigen receptor T-cell (CAR-T) therapy, which has shown remarkable success in hematologic malignancies. However, the application of these therapies to solid tumors, such as lung and colorectal cancers, has faced significant challenges.

Lipogenic enzyme FASN promotes mutant p53 accumulation and gain-of-function through palmitoylation.

The tumor-suppressive function of p53 is frequently disrupted by mutations in cancers. Missense mutant p53 (mutp53) protein often stabilizes and accumulates to high levels in cancers to promote tumorigenesis through the gain-of-function (GOF) mechanism. Currently, the mechanism of mutp53 accumulation and GOF is incompletely understood.

Photocatalytic degradation of methylene blue by CdS quantum dots biosynthesized by cysteine synthetase TtCsa1 from Tetrahymena thermophila.

HS is a crucial endogenous gaseous signaling molecule involved in various metabolic pathways and is associated with the biological response to heavy metal stress. The biomineralization of CdS serves as a critical mechanism for responding to cadmium stress and removing Cd in different organisms. CdS is also a widely utilized semiconductor material for the photocatalytic degradation of dyes.

Clinical Value of Hematological Inflammatory Indices in the Early Diagnosis of Neonatal Pneumonia.

Background: Neonatal pneumonia is a common and serious infectious disease in the neonatal period, particularly affecting preterm, low birth weight, and immunodeficient neonates, and poses a significant threat to their life and health. Finding convenient, reliable, and minimally invasive biomarkers is a key focus of clinical research. This study investigated the clinical value of the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic inflammatory index (SII) in the early diagnosis of neonatal pneumonia.