Sulfated Polysaccharides Reduce the Adhesion of Nano-COM Crystals to Renal Epithelial Cells by Inhibiting Oxidative and Endoplasmic Reticulum Stress.

Adhesion between calcium oxalate crystals and renal tubular epithelial cells is a vital cause of renal stone formation; however, the drugs that inhibit crystal adhesion and the mechanism of inhibition have yet to be explored. The cell injury model was constructed using nano-COM crystals, and changes in oxidative stress levels, endoplasmic reticulum (ER) stress levels, downstream p38 MAPK protein expression, apoptosis, adhesion protein osteopontin expression, and cell-crystal adhesion were examined in the presence of polysaccharide (DLP) and sulfated DLP (SDLP) under protected and unprotected conditions. Both DLP and SDLP inhibited nano-COM damage to human kidney proximal tubular epithelial cell (HK-2), increased cell viability, decreased ROS levels, reduced the opening of mitochondrial membrane permeability transition pore, markedly reduced ER Ca ion concentration and adhesion molecule OPN expression, down-regulated the expression of ER stress signature proteins including CHOP, Caspase 12, and p38 MAPK, and decreased the apoptosis rate of cells.

Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals.

Purpose: The crystal adhesion caused by the damage of renal tubular epithelial cells (HK-2) is the key to the formation of kidney stones. However, no effective preventive drug has been found. This study aims to explore the recovery effects of four Laminaria polysaccharides (SLPs) with different sulfate (-OSO) contents on damaged HK-2 cells and the difference in the adhesion of damaged cells to nanometer calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) before and after recovery.

Antioxidant Activity of Polysaccharides with Different Molecular Weights and Cytotoxicity Difference of Polysaccharides Regulated CaOx to HK-2 Cells.

Objective: This study aimed to investigate the growth of calcium oxalate (CaOx) crystals regulated by polysaccharides (AAPs) with different viscosity-average molecular weights (), the toxicity of AAP-regulated CaOx crystals toward HK-2 cells, and the prevention and treatment capabilities of AAPs for CaOx stones.

Methods: The scavenging capability and reducing capacity of four kinds of AAPs ( of 31.52, 11.

Quantification of the renal sinus fat and exploration of its relationship with ectopic fat deposition in normal subjects using MRI fat fraction mapping.

Purpose: To determine the renal sinus fat (RSF) volume and fat fraction (FF) in normal Chinese subjects using MRI fat fraction mapping and to explore their associations with age, gender, body mass index (BMI) and ectopic fat deposition.

Methods: A total of 126 subjects were included in the analysis. RSF volume and FF, visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) area, and hepatic and pancreatic FFs were measured for each subject.

Synergistic inhibition of calcium oxalate crystal formation and synergistic protection of HK-2 cells from crystal damage by sulfated polysaccharide and potassium citrate.

: The first objective is to study the synergistic inhibition of calcium oxalate (CaOx) formation by polysaccharides (DLP and SDLP, before and after sulfation) and potassium citrate (Kcit) and determine the synergistic protection of renal epithelial cells (HK-2 cells) caused by CaOx crystal damage. The second objective is to explore new ways to prevent and treat kidney stones. : The CaOx crystals regulated by five additives (Kcit group, DLP group, SDLP group, DLP-Kcit synergistic group and SDLP-Kcit synergistic group) were characterized by FT-IR, XRD, SEM, zeta potential, ICP, and TGA.

Antioxidant Activities and Cytotoxicity of the Regulated Calcium Oxalate Crystals on HK-2 Cells of Polysaccharides from with Different Molecular Weights.

The antioxidant activities of seven degraded products (GLPs) with different molecular weights () of polysaccharides from were compared. The of GLP1-GLP7 were 106, 49.6, 10.

Carboxymethylation of Polysaccharide and Its Repair Effect on Damaged HK-2 Cells.

Objective: is the best traditional medicine for treating kidney calculi in China. This study is aimed at increasing the carboxyl (-COOH) content of polysaccharide (DSP0) and further increasing its antistone activity.

Methods: DSP0 was carboxymethylated with chloroacetic acid at varying degrees.

Magnetic Resonance Imaging Assessment of Abdominal Ectopic Fat Deposition in Correlation With Cardiometabolic Risk Factors.

Purpose: Ectopic fat accumulation and abdominal fat distribution may have different cardiometabolic risk profiles. This study aimed to assess the associations between various magnetic resonance imaging (MRI)-acquired fat depots and cardiometabolic risk factors.

Methods: A total of 320 subjects with median age of 59 years, 148 men and 172 women, were enrolled in the study.

Evaluation of the value of water retention service in the middle and upper reaches of Hunhe River based on SWAT Model.

The upper and middle reaches of the Hunhe River, with forest coverage rate up to 68%, is an important water source protection area in Liaoning Province. Based on the SWAT model and shadow engineering method, we aimed to estimate the function and service value of water retention in the upper and middle Hunhe River basin from 2000 to 2019, to analyze the spatial heterogeneity, and to provide a scientific basis for the ecological compensation and ecological restoration of the region. Our results showed that the function and service value of water retention in the upper and middle reaches of the Hunhe increased first, and then decreased from 2000 to 2019.

Longitudinal association between sleep and 5-year incident metabolic syndrome in older Chinese adults: a community-based cohort study.

Purpose: Our aim was to investigate the association between sleep and the development of metabolic syndrome (MetS) in Chinese older adults and to accumulate evidence for the prevention of MetS through sleep management.

Methods: This prospective study followed 3005 participants aged over 60 derived from the Weitang Geriatric Diseases Study who were without MetS at baseline. MetS was defined according to the Adult Treatment Panel III (ATP III) criteria.

White blood cell count as a mediator of the relationship between depressive symptoms and all-cause mortality: A community-based cohort study.

Background And Objectives: One possible pathway by which depressive symptoms influence all-cause mortality is through their relationship with markers of inflammatory response. We aimed to examine whether white blood cell (WBC) count mediated the relationship between the presence of depressive symptoms and all-cause mortality among older Chinese people.

Methods: This community-based cohort study targeted 4053 individuals aged 60 years or over.

Metabolic Syndrome and 5-Year Incident Hyperuricemia Among Older Chinese Adults: A Community-Based Cohort Study.

Background: There was a lack of studies focusing on older adults about the longitudinal association between metabolic syndrome (MetS) and hyperuricemia (HUA). We aimed to assess the association of baseline MetS and incident HUA among older Chinese adults, with a special focus on the associations between different combinations of MetS components and HUA.

Methods: Data of 3247 Chinese adults aged 60 years or older included in a community-based longitudinal cohort study were analyzed.

Elevated blood neutrophil to lymphocyte ratio in older adults with cognitive impairment.

Background: Previous studies have assessed the association of neutrophil to lymphocyte ratio (NLR) with cognitive impairment (COI) in clinical settings. Whether NLR is associated with COI among free-living seniors at population level remains unknown.

Objectives: We aimed to assess the relationship between NLR and COI among community-dwelling older adults and the predictive value of NLR for COI screening in the community.

Depressive symptoms and metabolic syndrome components among older Chinese adults.

Background: Few studies examined associations between depressive symptoms and metabolic syndrome (MetS) among older Chinese adults. Considering that the prevalence of depressive symptoms is high in older Chinese adults, we aimed to examine associations of depressive symptoms with MetS and its components in older Chinese adults.

Methods: Data from a community-based cross-sectional study of 4579 Chinese adults aged 60 years or older were analyzed.

Depressive symptom as a mediator of the influence of self-reported sleep quality on falls: a mediation analysis.

It is well known that sleep quality was associated with falls. This study aimed to examine whether the presence of depressive symptoms mediate the association of self-reported sleep quality with falls. Data of community-based study including 4,579 adults aged 60 years or older were analyzed.

Associations of sleep durations and sleep-related parameters with metabolic syndrome among older Chinese adults.

Purpose: There is a lack of data on the role of sleep in the development of metabolic syndrome (MetS) in older adults. We aimed to examine the associations of sleep durations at night and other sleep-related parameters with the presence of MetS in older Chinese adults.

Methods: Data of 4579 individuals aged 60 years or older from the Weitang Geriatric Disease Study were analyzed.

Disordered sleep and myopia among adolescents: a propensity score matching analysis.

Purpose: Observational studies have suggested a possible relationship between disordered sleep and myopia, but the association may be subject to selection biases. We aimed to minimize selection biases and assess the association by applying a propensity score matching (PSM) approach.

Methods: The study was designed as a school-based cross-sectional study on a Chinese cohort aged 13-14 years in rural areas of China.

Computed tomography imaging features of hepatic perivascular epithelioid cell tumor: A case report and literature review.

Rationale: Perivascular epithelioid cell tumor (PEComa) is a rare tumor which is most frequently found in uterus. The tumor arising from liver is extremely uncommon.

Patient Concerns: A 36-year-old female with abdominal distention, cramps, and low-grade fever for over 15 days.

Anticancer and Reversing Multidrug Resistance Activities of Natural Isoquinoline Alkaloids and their Structure-activity Relationship.

The severe anticancer situation as well as the emergence of multidrug-resistant (MDR) cancer cells has created an urgent need for the development of novel anticancer drugs with different mechanisms of action. A large number of natural alkaloids, such as paclitaxel, vinblastine and camptothecin have already been successfully developed into chemotherapy agents. Following the success of these natural products, in this review, twenty-six types of isoquinoline alkaloids (a total of 379 alkaloids), including benzyltetrahydroisoquinoline, aporphine, oxoaporphine, isooxoaporphine, dimeric aporphine, bisbenzylisoquinoline, tetrahydroprotoberberine, protoberberine, protopine, dihydrobenzophenanthridine, benzophenanthridine, benzophenanthridine dimer, ipecac, simple isoquinoline, pavine, montanine, erythrina, chelidonine, tropoloisoquinoline, azafluoranthene, phthalideisoquinoline, naphthylisoquinoline, lycorine, crinane, narciclasine, and phenanthridone, were summarized based on their cytotoxic and MDR reversing activities against various cancer cells.

Mass spectrometry-guided isolation of two new dihydrobenzophenanthridine alkaloids from Macleaya cordata.

Two new alkaloids 6-hydroxyethyldihydrochelerythrine (1) and 6-hydroxymethyl-7,8-demethylenedihydrochelerythrine (2) together with two analogues named maclekarpine E (3) and 6-hydroxymethyldihydrosanguinarine (4) were detected primarily from the leaves of Macleaya cordata by their characteristic mass spectrometry (MS) and tandem mass spectrometry (MS/MS). And then isolation of four targeted-compounds was performed by column chromatography and preparation HPLC under the guiding of MS. Finally, their structures were determined by spectrum analysis.

Detection of gallbladder stones by dual-energy spectral computed tomography imaging.

Aim: To evaluate the detectability of gallbladder stones by dual-energy spectral computed tomography (CT) imaging.

Methods: Totally 217 patients with surgically confirmed gallbladder stones were retrospectively analyzed who underwent single-source dual-energy CT scanning from August 2011 to December 2013. Polychromatic images were acquired.

Diagnostic Significance of Diffusion-Weighted MRI in Renal Cancer.

Background: This study aimed to investigate whether diffusion-weighted imaging (DWI) could contribute to the discrimination between benign and malignant renal cancer.

Methods: We searched the PubMed electronic database for eligible studies. STATA 12.

Apparent diffusion coefficient measurement by diffusion weighted magnetic resonance imaging is a useful tool in differentiating renal tumors.

Background: To determine the clinical value of apparent diffusion coefficient (ADC) measurement by diffusion weighted magnetic resonance imaging (DW-MRI) in differentiating renal tumors.

Methods: Electronic databases were searched using combinations of keywords and free words relating to renal tumor, ADC and DW-MRI. Based on carefully selected inclusion and exclusion criteria, relevant case-control studies were identified and the related clinical data was acquired.

Virtual non-contrast computer tomography (CT) with spectral CT as an alternative to conventional unenhanced CT in the assessment of gastric cancer.

Objective: The purpose of this study was to evaluate computed tomography (CT) virtual non-contrast (VNC) spectral imaging for gastric carcinoma.

Materials And Methods: Fifty-two patients with histologically proven gastric carcinomas underwent gemstone spectral imaging (GSI) including non-contrast and contrast-enhanced hepatic arterial, portal venous, and equilibrium phase acquisitions prior to surgery. VNC arterial phase (VNCa), VNC venous phase (VNCv), and VNC equilibrium phase (VNCe) images were obtained by subtracting iodine from iodine/water images.