Study on the correlation of electron spin resonance with pigment gallstones and trace Cu, Fe in diet.

Through the analysis of electron paramagnetic resonance (EPR) spectra from patients with pigment gallstones, it was found that both Fe and Cu signals were detected in the gallstone powder, exhibiting relatively high intensity. The EPR signal for Fe(III) showed g = 4.18 and ΔHpp = 4.

Preparation and application of chitosan/nano-TiO₂/daisy essential oil composite films in the preservation of .

Chitosan, a natural polysaccharide, is widely recognized for its biocompatibility, biodegradability, and film-forming properties, making it an ideal candidate for food preservation applications. This study focuses on the development of chitosan-based nanocomposite films incorporating nano-TiO and Daisy Essential Oil for the preservation of . The composite films were synthesized and characterized using FT-IR, XRD, SEM, TGA, UV-Visible spectroscopy, and contact angle measurements to evaluate their structural, thermal, and physical properties.

Ionic Liquid-Based Polarity-Adjustable Deep Eutectic Solvent Extraction Followed by High-Performance Liquid Chromatography-Diode-Array Detection for the Determination of Liposoluble Anthraquinones in Salvia miltiorrhiza Bge. Root.

A simple, green, and efficient ultrasound-assisted deep eutectic solvents (DESs) extraction followed by high-performance liquid chromatography-diode array detector was established to detect four liposoluble anthraquinones in Salvia miltiorrhiza Bge. root. In this study, a series of polarity-adjustable DESs were prepared using n-hexanol and tetrabutylammonium chloride, and their structure, density, viscosity, pH, and thermal stability were determined.

Structural Optimization of Microfluidic Chips for Enhancing Droplet Manipulation and Observation via Electrodynamics Simulation.

Digital microfluidic chips (DMCs) have shown huge potential for biochemical analysis applications due to their excellent droplet manipulation capabilities. The driving force is a critical factor for characterizing and optimizing the performance of droplet manipulation. Conducting numerical analysis of the driving force is essential for DMC design, as it helps optimize the structural parameters.

Enhanced antitumor efficacy of bispecific antibody blocking PD-L1 and LAG-3 with doxorubicin: mechanism and safety evaluation.

Purpose: Combination therapy has emerged as a leading trend in cancer treatment, having had a significant impact on the management of advanced-stage breast cancer. This approach, which relies on immune checkpoint modulation, has revolutionized the therapeutic landscape. However, the precise mechanisms underlying its therapeutic effects remain unclear.

Physiological resistance of Dendrocalamus brandisii culms to alcohol stress during living bamboo wine production process.

Background: Currently, living bamboo wine has gained immense popularity in China. However, the effects of alcohol on the physiological processes of bamboo during the production of living bamboo wine have not yet been investigated.

Methods: To investigate the resistance of bamboo culms to the alcohol-induced stress, we conducted a comprehensive analysis of various factors, including changes in cell wall chemical components, antioxidant defense systems, endogenous plant hormones and transcriptomics, both before and after the alcohol injection.

Genome-wide CRISPR screening identifies PHF8 as an effective therapeutic target for KRAS- or BRAF-mutant colorectal cancers.

Background: Mutations in KRAS and BRAF genes are prevalent in colorectal cancer (CRC), which strikingly promote tumorigenesis and lead to poor response to a variety of treatments including immunotherapy by activating the MAPK/ERK pathway. Thus, there is an urgent need to discover effective therapeutic targets and strategies.

Methods: CRISPR-Cas9 lentiviral knockout library was used to screen the suppressors of anti-PD1 immunotherapy.

Responsive hydrogel modulator with self-regulated polyphenol release for accelerating diabetic wound healing precise immunoregulation.

Nonhealing chronic wounds are intractable clinical complications of diabetes and are characterized by high protease activity, severe oxidative stress and sustained inflammatory response. In this case, the development of functional hydrogel dressings to modulate the immune microenvironment is a well-known strategy, where the precise stimuli-responsive and spatiotemporally controlled release of bioactive molecules remains a huge challenge. Herein, we developed responsive hydrogels with self-regulated bioactive molecule release based on the protease activity in diabetic wound sites, to serve as a smart immune microenvironment modulator for accelerating wound healing.

The modified combo i3 + 3 design for novel-novel combination dose-finding trials in oncology.

We consider a modified Ci3 + 3 (MCi3 + 3) design for dual-agent dose-finding trials in which both agents are tested on multiple doses. This usually happens when the agents are novel therapies. The MCi3 + 3 design offers a two-stage or three-stage version, depending on the practical need.

Urinary concentrations of phthalate metabolites and the survival of high-grade serous ovarian cancer with advanced stage.

Phthalates have been reported to increase the risk of various hormone-dependent cancers. However, there is still a lack of evidence regarding the association between phthalates and overall survival (OS) in advanced high-grade serous ovarian cancer (HGSOC). This study investigated the relationship between urinary phthalate metabolites and OS in patients with HGSOC using a nested case-control study within the Ovarian Cancer Follow-Up Study.

Associations between pre- and post-diagnosis dietary inflammatory patterns and ovarian cancer survival: results from the Ovarian Cancer Follow-Up Study.

Background: Dietary factors impact systemic inflammation, which not only correlates with poorer outcomes in patients with ovarian cancer (OC) but also promotes cancer development through increased cell division, genetic alterations, and malignant transformation of epithelial cells at inflammatory sites. However, evidence between dietary inflammatory patterns and OC survival remains sparse.

Objective: The aim of this study was to examine associations between pre- and post-diagnosis dietary inflammatory patterns, including their changes, and overall survival (OS).

Application of platelet-rich plasma lysate combined with hyaluronic acid microneedles for the treatment of alopecia.

Androgenetic alopecia (AGA) continues to pose a significant challenge due to the paucity of effective therapeutic options. Upon lysis, platelet-rich plasma (PRP) releases numerous growth factors (GFs), which facilitate tissue reconstruction and hair regeneration. However, concerns such as infection, bleeding, local erythema, and patient anxiety associated with injections have substantially diminished patient acceptance.

The influence of survival conditions on farmer willingness to participate in non-grain conversions of cultivated land based on the SOR model.

Non-grain conversions (NGC) of cultivated land pose a great threat to national food security, and farmers are the direct actors in the NGC of cultivated land. Exploring the influence of farmer' survival situation on their willingness to convert cultivated land to non-grain land can provide theoretical support for formulating control policies for NGC of cultivated land. Based on the data obtained from 650 questionnaires in Henan Province and using the SOR model as the theoretical basis, this study explored the impact mechanism of the survival situations of two types of farmers, namely, those with agricultural livelihoods (AL) and those with nonagricultural livelihoods (NL), on their willingness to convert farmland into non-grain land through the use of structural equation models.

Quality decline of prepared dishes stored at 4 °C: Microbial regulation of nitrite and biogenic amine formation.

Prepared dishes have high water content and complex nutritional composition, making them highly susceptible to microbial contamination and oxidative spoilage during storage. To elucidate the effects of microorganisms on the quality of prepared dishes and the formation of contaminants, the dishes "stewed pork with cabbage" were stored at 4 °C for 0, 1, 3, 5, and 7 days. Techniques such as high-performance liquid chromatography (HPLC), ion chromatography (IC), and 16S rRNA sequencing were used to explore changes in quality of the prepared dishes.

Efficient degradation of neomycin by and isolated from mangrove soil and pharmaceutical wastewater.

Neomycin, an aminoglycoside antibiotic, is widely utilized for veterinary medicine in disease prevention. Biodegradation is a key pathway for the removal of neomycin from the environment. To date, only the white-rot fungus and the ericoid mycorrhizal fungus have been documented to efficiently degrade neomycin.

Theaflavins Are Improved by the Oxidation of Catechins in Tannase Treatment During Black Tea Fermentation.

The treatment of black tea fermentation with different exogenous tannases was investigated, and processing parameters during black tea fermentation, including fermentation time, fermentation temperature, and exoenzyme amounts, were optimized, while the consumption and transformation pathways of catechins were analyzed. The results showed that tannase from was ultimately selected as the optimal enzyme to effectively increase the content of theaflavins by promoting the hydrolysis reaction and benzoylation reaction of catechins, resulting in a greater theaflavin (TF) content of 1.41%.

Characterization of the Gene Family in Blueberry ( L.) and the Role of VcSWEET6 Related to Sugar Accumulation in Fruit Development.

Sugars will eventually be exported transporters (SWEETs) are essential transmembrane proteins involved in plant growth, stress responses, and plant-pathogen interactions. Despite their importance, systematic studies on SWEETs in blueberries ( L.) are limited.

Application of chitosan/Nano-TiO₂/Daisy essential oil composite film for the preservation of Actinidia arguta: Inhibition of spoilage microorganisms and induction of resistance.

This study explores the inhibitory effects of a chitosan/nano-TiO₂/Daisy Essential Oil (CSTD) composite film on spoilage microorganisms affecting Actinidia arguta. Owing to its high nutritional value and water content, Actinidia arguta is highly susceptible to microbial spoilage, leading to a significantly shortened shelf life. Traditional chemical preservation methods are ineffective against microbial spoilage and raise concerns about safety and environmental sustainability, highlighting the demand for natural, effective alternatives.

Single-nucleus RNA sequencing decodes abnormal cell-collagen communication in a sheep endometrial fibrosis model.

Endometrial fibrosis in sheep reduces reproductive performance with elusive therapeutic targets. The fibrotic endometrium is a complex ecosystem with heterogeneous cells and their interactions. The molecular characterization of key cells and the mechanisms of these interactions are unclear.

Dysregulated Pathways During Pregnancy Predict Drug Candidates in Neurodevelopmental Disorders.

Maternal health during pregnancy has a direct impact on the risk and severity of neurodevelopmental disorders (NDDs) in the offspring, especially in the case of drug exposure. However, little progress has been made to assess the risk of drug exposure during pregnancy due to ethical constraints and drug use factors. We collected and manually curated sub-pathways and pathways (sub-/pathways) and drug information to propose an analytical framework for predicting drug candidates.

Association of pre- and post-diagnosis dietary total antioxidant capacity (TAC) and composite dietary antioxidant index (CDAI) with overall survival in patients with ovarian cancer: a prospective cohort study.

Background: The evidence on the relationship of dietary antioxidant nutrients with the survival of ovarian cancer (OC) remains scarce.

Objective: This study aimed to investigate these associations in a prospective cohort of Chinese patients with OC.

Methods: In this prospective cohort study, patients with epithelial OC completed a food frequency questionnaire at diagnosis and 12 months post-diagnosis, and were followed from 2015 to 2023.

Association between plasma perfluoroalkyl substances and high-grade serous ovarian cancer overall survival: A nested case-control study.

Background: Although evidence suggests that perfluoroalkyl and polyfluoroalkyl substances (PFASs) are positively correlated to several disease risks, no studies have proven if plasma PFASs are related to ovarian cancer survival.

Objective: To explore the association between plasma PFASs and high-grade serous ovarian cancer (HGSOC) overall survival (OS) in the population who did not smoke.

Methods: We conducted a nested case-control study within the Ovarian Cancer Follow-Up Study, matching 159 dead patients and 159 survival ones based on body mass index, sample date, and age at diagnosis.

Modification of black soybean (Glycine max(L.)merr.) residue insoluble dietary fiber with ultrasonic, microwave, high temperature and high-pressure, and extrusion.

Recent studies have emphasized the modification of Insoluble Dietary Fiber (IDF) to enhance its physicochemical properties and functional performance. This study systematically examined the effects of ultrasonic treatment, microwave irradiation, high-temperature and high-pressure processing, and screw extrusion on the physicochemical characteristics, in vitro antioxidant activity, and adsorption capacities of High-Purity Insoluble Dietary Fiber (HPIDF) derived from black bean residues. Although these physical modifications did not alter the functional group composition or crystalline structure of HPIDF, they significantly enhanced its porosity, water-holding capacity (WHC), oil-holding capacity (OHC), and adsorption capacities for glucose, cholesterol, bile salts, and metal ions.

Polydopamine nanoparticles loaded with sodium ferulate for targeted therapy of myocardial infarction in endothelial cells.

Myocardial infarction (MI) is a leading cause of heart failure and death in cardiovascular diseases. Most drug trials currently fail due to inadequate local drug activity and side effects. In this study, we developed a novel polydopamine (PDA) nano delivery system that carries sodium ferulate (SF) and is modified with RGD peptides (SF/RGD-PDA NPs) for precise targeted delivery.