Polymorphism Is Associated With Altered Systemic Inflammation and Adverse Trauma Outcomes.

Trauma is a leading cause of morbidity and mortality. It is unclear why some trauma victims follow a complicated clinical course and die, while others, with apparently similar injury characteristics, do not. Interpatient genomic differences, in the form of single nucleotide polymorphisms (SNPs), have been associated previously with adverse outcomes after trauma.

Effects and mechanism on the removal of neurotoxin β-N-methylamino-l-alanine (BMAA) by chlorination.

β-N-Methylamino-l-alanine (BMAA), a new cyanobacterial toxin, is found in different aquatic ecosystems worldwide and is to threaten the human nervous system. Therefore, it is important for water plants to develop feasible methods to counter the effects of BMAA. In this study, the removal of BMAA by chlorine, as well as its intermediate products, at different pH values and the mechanism of pH on the removal BMAA were investigated.

Impact factors on the production of β-methylamino-L-alanine (BMAA) by cyanobacteria.

Cyanobacteria produce a series of secondary metabolites, one of which is beta-N-methylamino-l-alanine (BMAA). BMAA is considered to be the cause of human neurodegeneration. Compared with other cyanotoxins, the role of BMAA in cyanobacteria remains unclear.

Magnetically stabilized bed packed with synthesized magnetic silicone loaded with ionic liquid particles for efficient enrichment of flavonoids from tree peony petals.

In this work, synthesized magnetic silicone loaded with ionic liquid (FeO@SiO@IL) particles combined with gas-liquid-solid magnetically stabilized bed (GLS-MSB) were applied to enrich flavonoids from tree peony petal extraction solution. The magnetic core (FeO) encased in silica was conducive to its rapid and efficient separation, and the modification of silica with ionic liquids (ILs) could provide the functional groups for selective adsorption of flavonoids. Furthermore, the magnetic materials were evenly dispersed in the GLS-MSB system, realizing the adequate contact and causing the positive influence on the result.

Comparative Transcriptome Analysis Reveals the Cause for Accumulation of Reactive Oxygen Species During Pollen Abortion in Cytoplasmic Male-Sterile Kenaf Line 722HA.

Cytoplasmic male sterility (CMS) is a maternally inherited trait used for hybrid production in plants, a novel kenaf CMS line 722HA was derived from the thermo-sensitive male-sterile mutant 'HMS' by recurrent backcrossing with 722HB. The line 722HA has great potential for hybrid breeding in kenaf. However, the underlying molecular mechanism that controls pollen abortion in 722HA remains unclear, thus limiting the full utilization of this line.

A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms.

In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant's tissue (1-10 μg) to 20 μl 0.

Structural Characterization and Antioxidant Activity of Polysaccharides from (Doll.) Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway.

Unlabelled: The purpose of this study was to characterize the polysaccharides from (Doll.) Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.

Methods: A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and H nuclear magnetic resonance (H NMR) spectroscopy were carried out to characterize AMC polysaccharides.

sp. nov., isolated from mountain soil.

A Gram-stain-negative, strictly aerobic, motile, yellow, rod-shaped bacterium, designated ZDH117, was isolated from soilsampled atthe Danxialandformin Guangdong Province, PR China. The 16S rRNA gene sequence of strain ZDH117 had highest similarityvalues to DSM 7418 (97.5 %), CP1D (97.

The effects of hyperuricemia on the differentiation and proliferation of osteoblasts and vascular smooth muscle cells are implicated in the elevated risk of osteopenia and vascular calcification in gout: An in vivo and in vitro analysis.

Background: In this study, we established both an animal model and a cellular model of hyperuricemia (HUC). Subsequently, we treated these models with allopurinol (ALLO) to study the effect of uric acid (UA) and ALLO on the differentiation and proliferation of osteoblasts and vascular smooth muscle cells (VSMC).

Methods: Western Blot, immunohistochemistry assay, and real-time polymerase chain reaction were conducted to measure the changes in the expression of differentiation-related factors in osteoblasts and VSMCs in HUC and HUC+ALLO groups.

Efficient Homogenization-Ultrasound-Assisted Extraction of Anthocyanins and Flavonols from Bog Bilberry ( L.) Marc with Carnosic Acid as an Antioxidant Additive.

To explore the optimum conditions for the extraction of anthocyanins and flavonols from bog bilberry ( L.) marc on a single-factor experimental basis, a response surface methodology was adopted for this intensive study. The extraction procedure was carried out in a Waring blender and followed an ultrasonic bath, and the natural antioxidant carnosic acid was added to inhibit oxidation.

Identification of Key Genes and Candidated Pathways in Human Autosomal Dominant Polycystic Kidney Disease by Bioinformatics Analysis.

Background/aims: Autosomal dominant polycystic kidney disease (ADPKD) is the most common genetic form of kidney disease. High-throughput microarray analysis has been applied for elucidating key genes and pathways associated with ADPKD. Most genetic profiling data from ADPKD patients have been uploaded to public databases but not thoroughly analyzed.

Development of a Concise Rhodamine-Formylhydrazine Type Fluorescent Probe for Highly Specific and Ultrasensitive Tracing of Basal HOCl in Live Cells and Zebrafish.

Hypochlorous acid (HOCl) has received special attention by virtue of its pivotal antimicrobial nature, and the appropriate amount of HOCl is beneficial to innate immunity of host to cope with microbial invasion. However, the uncontrollable accumulation of HOCl is implicated in many human diseases and even cancers. Thus, to determine its deeper biological functions, it is significantly important to specifically monitor intracellular HOCl in biosystems.

Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium.

Tremella fuciformis is an edible and medicinal white jelly mushroom. It has a life cycle that interacts with its companion fungus Annulohypoxylon stygium, both in natural conditions and artificial cultivation. RNA sequencing (RNA-Seq) was used to study the interaction between T.

Stirring revealed new functions of ethylenediamine and hydrazine in the morphology control of copper nanowires.

Cu nanowires, as promising candidates in many fields because of their merits, are commonly prepared by the solution phase based synthesis which is a simple and scalable method. However, precise control of the morphology, particularly surface roughness, of Cu nanowires is still challenging; and moreover, detailed formation mechanisms of Cu nanowires, in solution phase based synthesis, are still unclear. We here show the morphology manipulation of Cu nanowires by adjusting the stirring rate and the amounts of ethylenediamine and hydrazine (N2H4), yielding Cu nanowires with either smooth or rough surface.

Application of contrast-enhanced ultrasound for scar pregnancy cases misdiagnosed as other diseases.

Objective: To demonstrate the feasibility of utilizing contrast-enhanced ultrasound (CEUS) in scar pregnancies misdiagnosed by transvaginal ultrasound (TVS).

Methods: CEUS was performed in three patients with clinically suspected abnormal pregnancy, and the diagnosis were inconclusive by TVS. Clinical manifestations and features of TVS and CEUS in these cases were analyzed.

Striatisporolide A, a butenolide metabolite from Athyrium multidentatum (Doll.) Ching, as a potential antibacterial agent.

The present study aimed to investigate the antibacterial activity of striatisporolide A (SA) against Escherichia coli (E. coli) and the underlying mechanism. Antibacterial activity was evaluated according to the inhibitory rate and zone of inhibition.

Dirty-data-based alarm prediction in self-optimizing large-scale optical networks.

Machine-learning-based solutions are showing promising results for several critical issues in large-scale optical networks. Alarm (caused by failure, disaster, etc.) prediction is an important use-case, where machine learning can assist in predicting events, ahead of time.

MicroRNA-451 inhibits vascular smooth muscle cell migration and intimal hyperplasia after vascular injury via Ywhaz/p38 MAPK pathway.

Increasing evidence has indicated that intimal hyperplasia is a common event in the pathophysiology of many vascular diseases including atherosclerosis (AS). Recently, deregulated microRNAs (miRNAs) have been reported to be associated with the pathophysiology of AS. However, the biological function and regulatory mechanisms of miRNAs in intimal hyperplasia in AS remain largely unclear.

RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A.

Background: Pollen development is an energy-consuming process that particularly occurs during meiosis. Low levels of adenosine triphosphate (ATP) may cause cell death, resulting in CMS (cytoplasmic male sterility). DNA sequence differences in ATP synthase genes have been revealed between the N- and S-cytoplasms in the cotton CMS system.

ASSOCIATION BETWEEN SERUM URIC ACID LEVEL AND BODY MASS INDEX IN SEX- AND AGE-SPECIFIC GROUPS IN SOUTHWESTERN CHINA.

To investigate the sex- and age-specific association between serum uric acid level and body mass index (BMI). A total of 144,856 subjects aged 20 to 79 years were enrolled in this cross-sectional study. Serum uric acid level, renal function, hepatic function, and lipid profile were investigated.

Coaxial differential dynamic microscopy for measurement of Brownian motion in weak optical field.

Weak optical fields cause less damage to active cells and are easier to realize than traditional and tightly focused optical fields. While these fields are promising for biomedical science and particle manipulation applications, they lack a method for precise particle diffusion measurement because the weak fields cause the small changes in particle motion caused by weak fields. In this paper, we present a coaxial differential dynamic microscopy (CDDM) technique that uses a differential dynamic microscopy system, combined with an adjustable optical field.

L-NBP, a multiple growth factor activator, attenuates ischemic neuronal impairments possibly through promoting neuritogenesis.

In China, L-3-n-butylphthalide (L-NBP) showed promising pharmacological actions in stroke treatment. Analyzing the characteristics of L-NBP might provide valuable hints for new drug design. The current study is aimed to determine the effects of L-NBP on neuritogenesis and further to elucidate the neuronal protection against stroke impairment in vitro.

Efficient Transformation of the White Jelly Mushroom Tremella fuciformis (Tremellomycetes) and Its Companion Fungus Annulohypoxylon stygium (Ascomycetes) Mediated by Agrobacterium tumefaciens.

Tremella fuciformis is an edible white jelly mushroom with medicinal qualities. The formation of T. fuciformis fruiting bodies is highly dependent on the presence of Annulohypoxylon stygium under natural conditions and during artificial cultivation.

Identification of Mineralocorticoid Receptor Modulators with Low Impact on Electrolyte Homeostasis but Maintained Organ Protection.

The mechanism-based risk for hyperkalemia has limited the use of mineralocorticoid receptor antagonists (MRAs) like eplerenone in cardio-renal diseases. Here, we describe the structure and property-driven lead generation and optimization, which resulted in identification of MR modulators ( S)-1 and ( S)-33. Both compounds were partial MRAs but still demonstrated equally efficacious organ protection as eplerenone after 4 weeks of treatment in uni-nephrectomized rats on high-salt diet and aldosterone infusion.

The ultrasonic whirlpool sign combined with plasma d-dimer level in adnexal torsion.

Objectives: The whirlpool sign (WS) and plasma d-dimer are used as a sonographic marker and laboratory index for adnexal torsion (AT), respectively. The aim of this study was to evaluate whether their combination improved diagnostic efficiency of AT in patients presenting with a benign ovarian mass and abdominal pain and clinically suspected AT.

Materials And Methods: Fifty-four patients with clinically suspected AT with benign ovarian mass and abdominal pain were analyzed and divided into an AT group (n = 28) and control group (n = 26).