A pediatric virtual care evaluation framework and its evolution using consensus methods.

Background: The use of virtual care has increased dramatically in response to the COVID-19 pandemic, yet evidence is lacking regarding the impact of virtual care on patient outcomes, particularly in pediatrics. A standardized evaluation approach is required to support the integration of virtual care into pediatric health care delivery programs. The objective of this work was to develop a comprehensive and structured framework for pediatric virtual care evaluation.

[Plexiform neurofibroma of the upper eyelid and orbit in a patient with von Recklinghausen's disease--case report].

Neurofibromatosis type 1 is an autosomal dominant genetic disease, which belongs to the neuro-oculo-cutaneous syndromes (phakomatoses). The authors present a case of a girl with familial neurofibromatosis. In addition to typical skin lesions and Lisch nodules on the iris, the plexiform neurofibroma of the upper eyelid and orbit as well as hamartoma of the central nervous system were observed.

[Treatment results of squint and incorrect fixation in infants].

Purpose: Analysis of the results of medical examination and treatment of infants with squint and undeveloped fixation reflex, and with nystagmus.

Material And Methods: 215 babies with these distempers were classified to the treatment. The children were included into five prophylactic groups: group 1-66 babies with eccentric fixation and without squint or with periodic squint; group 2-60 babies with acentric fixation and with manifested convergent squint at a visual angle from 25 degrees to 45 degrees (average 35 degrees); group 3-55 babies with correct fixation and with manifested convergent squint at a visual angle from 25 degrees to 45 degrees (average 35 degrees), and with divergent squint at a visual angle from 5 degrees to 20 degrees (average 12.

[Treatment of infantile strabismus with eye movement disorders].

Purpose: Possibilities of application of localization method in the treatment of infantile strabismus in a very early period are presented.

Material And Methods: The authors applied treatment with hypercorrective and compensating prisms in 17 children with infantile strabismus. The age of the children ranged from 0.

Physiological suppression of superoxide dismutase deficiency in yeast Saccharomyces cerevisiae.

Deficiency in superoxide dismutases results in pleiotropic effects including hypersensitivity to oxygen and amino acid auxotrophy. Various types of physiological suppressors of deficiency in cytosolic superoxide dismutase were isolated, whereas attempts to isolate suppressors of mitochondrial enzyme deficiency proved a failure. General characteristics of isolated suppressors are presented.

[Prevention of amblyopia by active production of the macular fixation reflex in infants].

Among 1455 examined babies aged 3 months the authors found a lack of normal fixation reflex in both eyes in 155 and in 52 in one eye only. In these eyes the parents applied 3 times a day an alternate occlusion of eyes or of the master eye only showing the babies interesting toys. After 2 weeks 83 babies showed normalization of fixation and after following 2 weeks of over described occlusion only 8 of them did not reveal a normal fixation reflex.

Superoxide dismutase deficiency and the toxicity of the products of autooxidation of polyunsaturated fatty acids in yeast.

Mutants of Saccharomyces cerevisiae, deficient in cytosolic superoxide dismutase and catalase activities were used to study the role of various oxygen species in the process of lipid peroxidation in yeast cells. Lipid peroxidation does not occur normally in yeast, because this organism is unable to form fatty acids with more than one double bond, whereas under physiological conditions, only fatty acids with at least two double bonds undergo this process. The fatty acid content of cellular lipids was modified by growing the cells in anoxia in the presence of oleic or linolenic acid.

Is hydroxyl radical generated by the Fenton reaction in vivo?

Yeast mutants deficient in activities of cytosolic superoxide dismutase and catalase A and T were exposed to four different kinds of oxygen stress. The response of the cells contradicts suggestions, that hydroxyl radical is formed in vivo through the Fenton reaction. The results suggest that superoxide radicals are directly responsible for cytotoxic effects of oxygen.

The role of respiratory chain in paraquat toxicity in yeast.

Yeast respiratory deficient mutants are resistant to paraquat. Similar resistance is caused by lowering the respiration by physiological mechanisms, as well as by some inhibitors of electron transfer chain of mitochondria. Presented results suggest that the major contribution of mitochondria to overall toxicity of paraquat in yeast is a consequence of very low level of cytochrome P-450, and presumably its presumably its reductase in aerobic yeast cells.

Catalase T deficient mutants of Saccharomyces cerevisiae.

A method for the isolation of catalase T deficient mutants of Saccharomyces cerevisiae is described. Ten mutants lacking catalase T and belonging to 5 complementation groups were isolated. CTT1 locus was identified as the structural gene for catalase T.

Characterization of two mutant strains of Saccharomyces cerevisiae deficient in coproporphyrinogen III oxidase activity.

Two new haem-deficient mutants of Saccharomyces cerevisiae were isolated on the basis of their catalase deficiency. Mutant H11 accumulated and excreted coproporphyrin III and was completely deficient in haem; the cell-free extract had no coproporphyrinogen oxidase activity. Mutant H12 accumulated uroporphyrin to coproporphyrin III and excreted coproporphyrin III, and contained a small amount of haem; the cell-free extract had a residual coproporphyrinogen oxidase activity.

Primary and secondary immune response to measles vaccine strain.

The rates of primary and secondary cell-mediated immune responses and their correlation with humoral responses were estimated. Cell-mediated reactions were evaluated by the leucocyte migration inhibition test. No significant differences were found in the intensity of primary and secondary cellular responses.

Hemoprotein formation in yeast. VII. Genetic analysis of pleiotropic mutants affected in the response to glucose repression and anoxia.

Genetical analysis of three pleiotropic mutants with changed regulation of hemoprotein level was performed. It was proved that single recessive mutations cgr4, cas1 and cgh1 are responsible for simultaneous changes of catalase T, cytochrome c oxidase and of some other enzyme levels, in glucose repression conditions. Two mutations cgr4 and cas1, responsible for glucose resistance of hemoprotein formation in aerobic conditions enable also anaerobic synthesis of catalase T.

Analysis of heme biosynthesis in catalase and cytochrome deficient yeast mutants.

Mutants of Saccharomyces cerevisiae, described as catalase and cytochromes deficient (Pachecka et al., 1974), have been analyzed for heme biosynthesis ability. Some enzymatic activities involved in protoheme synthesis were measured in acellular extracts, whereas whole cells were analyzed for cytochrome spectra and for possible accumulation of porphyrin synthesis intermediates.

Haemoprotein formation in yeast. II. Isolation of catalase regulatory mutants.

A procedure was described for the isolation of mutants affected in the regulation of catalase activity. Two such mutants, cgr 1 and cgr 2 were obtained. Both of them show catalase activity that is resistant to repression by glucose, but is sensitive to anoxia to the same extent as the wild type.

Macromolecular synthesis during conjugation in yeast.

Morphological changes and synthesis of DNA, RNA and protein during conjugation of Saccharomyces cerevisiae was studied. It was found that during conjugation distinct cell expansion and increase in the dry mass of the cell takes place. DNA synthesis is inhibited during mating while synthesis of RNA and protein is not affected.