Enhanced spinal cord repair using bioengineered induced pluripotent stem cell-derived exosomes loaded with miRNA.

Background: A spinal cord injury (SCI) can result in severe impairment and fatality as well as significant motor and sensory abnormalities. Exosomes produced from IPSCs have demonstrated therapeutic promise for accelerating spinal cord injury recovery, according to a recent study.

Objective: This study aims to develop engineered IPSCs-derived exosomes (iPSCs-Exo) capable of targeting and supporting neurons, and to assess their therapeutic potential in accelerating recovery from spinal cord injury (SCI).

Strategically Engineered Ru(II) Complexes with Enhanced ROS Activity Enabling Potent Sonodynamic Effect against Multidrug-Resistant Biofilms.

Sonodynamic therapy (SDT) can generate reactive oxygen species (ROS) to combat multidrug-resistant biofilms, which pose significant challenges to human health. As the key to producing ROS in SDT, the design of sonosensitizers with optimal molecular structures for sufficient ROS generation and activity in complex biofilm matrix is essential. In this study, we propose a π-expansion strategy and synthesize a series of small-molecule metal Ru(II) complexes (-) as sonosensitizers (-) to enhance the efficacy of SDT.

De Novo Designed Ru(II) Metallacycle as a Microenvironment-Adaptive Sonosensitizer and Sonocatalyst for Multidrug-Resistant Biofilms Eradication.

Albeit sonodynamic therapy (SDT) has achieved encouraging progress in microbial sterilization, the scarcity of guidelines for designing highly effective sonosensitizers and the intricate biofilm microenvironment (BME), substantially hamper the therapeutic efficacy against biofilm infections. To address the bottlenecks, we innovatively design a Ru(II) metallacycle-based sonosensitizer/sonocatalyst (named Ru-A3-TTD) to enhance the potency of sonotherapy by employing molecular engineering strategies tailored to BME. Our approach involves augmenting Ru-A3-TTD's production of ultrasonic-triggered reactive oxygen species (ROS), surpassing the performance of commercial sonosensitizers, through a straightforward but potent π-expansion approach.

Longitudinal study of mental health changes in residents affected by an initial outbreak of COVID-19 in China.

Introduction: The COVID-19 pandemic is ongoing, and the world continues to work to defeat it. We designed this study to understand the longitudinal change in the mental health of residents who experienced the initial disease outbreak in China and to explore the long-term influencing factors.

Methods: The Perceived Stress Scale (PSS), Generalized Anxiety Scale (GAD-7), and Patient Health Questionnaire-9 (PHQ-9) were administered to the same sample four times: during the initial outbreak (T1), 1 month later (T2), 18 months later (T3), and 26 months later (T4).

Aspartic acid and epidermal growth factor modified decellularized rabbit conjunctiva for conjunctival reconstruction.

Conjunctival reconstruction is an indispensable part of ocular surface regeneration. Decellularized matrix has been considered as an ideal conjunctival substitution for conjunctival reconstruction. In the present study, we report the use of a decellularized rabbit conjunctiva (DRC) for conjunctival reconstruction in the rabbit surgical trauma model.

Workplace violence against Chinese health professionals 2013-2021: A study of national criminal judgment documents.

Objectives: Patient-initiated hospital violence is a global problem which threatens the safety of health professionals and is indicative of doctor-patient tensions, impeding health system quality and access. The current study aimed to improve the understanding of medical workplace violence (WPV) in China, using authoritative and nationally representative judgment records, and to approach violence prevention strategies.

Methods: All litigation records relating to violence against health professionals between 2013 and 2021 were extracted from the China Judgment Online System.

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction.

Seeking for suitable conjunctival reconstruction substitutes to overcome the limitations of current substitutes, such as amniotic membrane, is urgent. Decellularized tissues have become a promising strategy for tissue engineering. In this study, we prepared decellularized porcine pericardium (DPP) scaffolds by the phospholipase A2 method and crosslinked them with aspartic acid (Asp) and human endothelial growth factor (hEGF) to enhance biological performance on the DPP, obtaining DPP-Asp-hEGF scaffolds.

Evaluation of Abramowitz functions in the right half of the complex plane.

A numerical scheme is developed for the evaluation of Abramowitz functions in the right half of the complex plane. For = - 1, … , 2, the scheme utilizes series expansions for ∣∣ < 1, asymptotic expansions for ∣∣ > with determined by the required precision, and least squares Laurent polynomial approximations on each sub-region in the intermediate region 1 ≤ ∣∣ ≤ . For > 2, is evaluated via a forward recurrence relation.

Theory of the Lattice Boltzmann method: Derivation of macroscopic equations via the Maxwell iteration.

We propose using the Maxwell iteration to derive the hydrodynamic equations from the lattice Boltzmann equation (LBE) with an external forcing term. The proposed methodology differs from existing approaches in several aspects. First, it need not explicitly introduce multiple-timescales or the Knudsen number, both of which are required in the Chapman-Enskog analysis.

Accuracy of the viscous stress in the lattice Boltzmann equation with simple boundary conditions.

Based on the theory of asymptotic analysis, we prove that the viscous stress tensor computed with the lattice Boltzmann equation (LBE) in a two-dimensional domain is indeed second-order accurate in space. We only consider simple bounce-back boundary conditions which can be reduced to the periodic boundary conditions by using the method of image. While the LBE with nine velocities on two-dimensional square lattice (i.

Reply to "Comment on 'Numerics of the lattice Boltzmann method: Effects of collision models on the lattice Boltzmann simulations'".

This Reply addresses two issues raised in the Comment [Phys. Rev. E 84, 068701 (2011)] by Karlin, Succi, and Chikatamarla (KSC): (1) A lattice Boltzmann (LB) model, which is claimed to have an H theorem, is not qualified to be called an entropic lattice Boltzmann equation (ELBE); and (2) the real ELBE with a variable relaxation time performs exceedingly well, as exhibited by their simulations of decaying "Kida vortex" flow in a three-dimensional periodic cube free of no-slip boundary.

Comment on "Heat transfer and fluid flow in microchannels and nanochannels at high Knudsen number using thermal lattice-Boltzmann method".

In this Comment we reveal the falsehood of the claim that the lattice Bhatnagar-Gross-Krook (BGK) model "is capable of modeling shear-driven, pressure-driven, and mixed shear-pressure-driven rarified [sic] flows and heat transfer up to Kn=1 in the transitional regime" made in a recent paper [Ghazanfarian and Abbassi, Phys. Rev. E 82, 026307 (2010)].

Numerics of the lattice Boltzmann method: effects of collision models on the lattice Boltzmann simulations.

We conduct a comparative study to evaluate several lattice Boltzmann (LB) models for solving the near incompressible Navier-Stokes equations, including the lattice Boltzmann equation with the multiple-relaxation-time (MRT), the two-relaxation-time (TRT), the single-relaxation-time (SRT) collision models, and the entropic lattice Boltzmann equation (ELBE). The lid-driven square cavity flow in two dimensions is used as a benchmark test. Our results demonstrate that the ELBE does not improve the numerical stability of the SRT or the lattice Bhatnagar-Gross-Krook (LBGK) model.

Effects of multitemperature nonequilibrium on compressible homogeneous turbulence.

We study the effects of the rotational-translational energy exchange on the compressible decaying homogeneous isotropic turbulence (DHIT) in three dimensions through direct numerical simulations. We use the gas-kinetic scheme coupled with multitemperature nonequilibrium based on the Jeans-Landau-Teller model. We investigate the effects of the relaxation time of rotational temperature, ZR, and the initial ratio of the rotational and translational temperatures, TR0/TL0, on the dynamics of various turbulence statistics including the kinetic energy K(t), the dissipation rate epsilon(t), the energy spectrum E(k,t), the root mean square of the velocity divergence theta'(t), the skewness Su(t) and the flatness Fu(t) of the velocity derivatives, and the probability distribution functions of the local Mach number Ma and the shocklet strength chi.

Gas-kinetic schemes for direct numerical simulations of compressible homogeneous turbulence.

We apply the gas-kinetic scheme (GKS) for the direct numerical simulations (DNSs) of compressible decaying homogeneous isotropic turbulence (DHIT). We intend to study the accuracy, stability, and efficiency of the gas-kinetic scheme for DNS of compressible homogeneous turbulence depending on both flow conditions and numerics. In particular, we study the GKS with multidimensional, quasi-one-dimensional, dimensional-splitting, and smooth-flow approximations.

Comment on "Alternative approach to the solution of the dispersion relation for a generalized lattice Boltzmann equation".

Recently Reis and Phillips [Phys. Rev. E 77, 026702 (2008)] proposed a perturbative method to solve the dispersion equation derived from the linearized lattice Boltzmann equation.

Theory of the lattice Boltzmann equation: symmetry properties of discrete velocity sets.

The lattice Boltzmann equation replaces continuous particle velocity space by a finite set; the velocity distribution function then varies over a finite-dimensional vector space instead of over an infinite-dimensional function space. The number of linearly independent moments of the distribution function in a lattice Boltzmann model cannot exceed the number of velocities; finite dimensionality therefore necessarily induces linear dependences among the moments that do not exist in a continuous theory. Given a finite velocity set, it is important to know which moments are free of these dependences.

Multiple-relaxation-time lattice Boltzmann models in three dimensions.

This article provides a concise exposition of the multiple-relaxation-time lattice Boltzmann equation, with examples of 15-velocity and 19-velocity models in three dimensions. Simulation of a diagonally lid-driven cavity flow in three dimensions at Re = 500 and 2000 is performed. The results clearly demonstrate the superior numerical stability of the multiple-relaxation-time lattice Boltzmann equation over the popular lattice Bhatnagar-Gross-Krook equation.

Lattice Boltzmann simulations of decaying homogeneous isotropic turbulence.

Decaying homogeneous isotropic turbulence in inertial and rotating reference frames is investigated to evaluate the capability of the lattice Boltzmann method in turbulence. In the inertial frame case, the decay exponents of kinetic energy and dissipation and the low wave-number scaling of the spectrum are studied. The results are in agreement with classical ones.

Theory of the lattice Boltzmann method: acoustic and thermal properties in two and three dimensions.

The focus of the present work is to provide an analysis for the acoustic and thermal properties of the energy-conserving lattice Boltzmann models, and a solution to the numerical defects and instability associated with these models in two and three dimensions. We discover that a spurious algebraic coupling between the shear and energy modes of the linearized evolution operator is a defect universal to the energy-conserving Boltzmann models in two and three dimensions. This spurious mode coupling is highly anisotropic and may occur at small values of wave number k along certain directions, and it is a direct consequence of the following key features of the lattice Boltzmann equation: (1) its simple spatial-temporal dynamics, (2) the linearity of the relaxation modeling for collision operator, and (3) the energy-conservation constraint.

Nonexistence of H theorems for the athermal lattice Boltzmann models with polynomial equilibria.

We prove that no H theorem exists for the athermal lattice Boltzmann equation with polynomial equilibria satisfying the conservation laws exactly and explicitly. The proof is demonstrated by using the seven-velocity model in a triangular lattice in two dimensions, and can be readily extended to other lattice Boltzmann models in two and three dimensions. Some issues pertinent to the numerical instabilities of the lattice Boltzmann method are discussed.