Publications by authors named "Lisha Zhu"

Constructed wetlands (CWs), a cost-effective and eco-friendly wastewater treatment technology, are extensively applied in various types of wastewater treatment. There is a series of strong impacts on CWs performance by the accumulation of clogging matters which attribute to physical, chemical, and biological processes after the long-term operation. This paper summarizes the mechanism of clogging formation, which can be classified into physical, chemical, and biological clogging.

View Article and Find Full Text PDF

Tendon and ligament injuries are the most common musculoskeletal injuries, and their regeneration is a complex process due to the poor natural healing ability of these tissues. The current therapies for tendon repair are limited in efficacy and their cellular and molecular mechanisms remain unclear. In this study, we identified AMP-activated protein kinase (AMPK) as a markedly upregulated factor in newborn tendons with high regenerative capacity.

View Article and Find Full Text PDF

DNA methylation is an epigenetic modification process that can alter the functionality of a genome. It has been reported to be a key regulator of fruit ripening. In this study, the DNA methylation changes of CpG islands of ethylene signaling genes regulated by 1-methylcyclopropene (1-MCP) during ripening and senescence of tomato fruit were detected.

View Article and Find Full Text PDF

The fallopian tube undergoes extensive molecular changes during the menstrual cycle and menopause. We use single-cell RNA and ATAC sequencing to construct a comprehensive cell atlas of healthy human fallopian tubes during the menstrual cycle and menopause. Our scRNA-seq comparison of 85,107 pre- and 46,111 post-menopausal fallopian tube cells reveals substantial shifts in cell type frequencies, gene expression, transcription factor activity, and cell-to-cell communications during menopause and menstrual cycle.

View Article and Find Full Text PDF
Article Synopsis
  • Anthracnose, caused by the fungus Colletotrichum gloeosporioides, significantly leads to postharvest losses in mango fruits.
  • The study showed that applying melatonin (MT) at a concentration of 1 mmol L can limit the spread of anthracnose while boosting plant defense mechanisms, including several enzyme activities and the production of phenolic compounds.
  • MiWRKY45, a transcription factor found to increase expression in response to MT and the fungal infection, plays a key role in activating the phenylpropanoid pathway, thus enhancing mango resistance to anthracnose.
View Article and Find Full Text PDF

The vast potential of medical big data to enhance healthcare outcomes remains underutilized due to privacy concerns, which restrict cross-center data sharing and the construction of diverse, large-scale datasets. To address this challenge, we developed a deep generative model aimed at synthesizing medical data to overcome data sharing barriers, with a focus on breast ultrasound (US) image synthesis. Specifically, we introduce CoLDiT, a conditional latent diffusion model with a transformer backbone, to generate US images of breast lesions across various Breast Imaging Reporting and Data System (BI-RADS) categories.

View Article and Find Full Text PDF

Transcriptome-wide association studies (TWAS) help identify disease causing genes, but often fail to pinpoint disease mechanisms at the cellular level because of the limited sample sizes and sparsity of cell-type-specific expression data. Here we propose scPrediXcan which integrates state-of-the-art deep learning approaches that predict epigenetic features from DNA sequences with the canonical TWAS framework. Our prediction approach, ctPred, predicts cell-type-specific expression with high accuracy and captures complex gene regulatory grammar that linear models overlook.

View Article and Find Full Text PDF

Aging impairs bone marrow mesenchymal stem cell (BMSC) functions as well as associated angiogenesis which is critical for bone regeneration and repair. Hence, repairing bone defects in elderly patients poses a formidable challenge in regenerative medicine. Here, the engineered dental pulp stem cell-derived exosomes loaded with the natural derivative of adenosine Cordycepin (CY@D-exos) are fabricated by means of the intermittent ultrasonic shock, which dually rejuvenates both senescent BMSCs and endothelial cells and significantly improve bone regeneration and repair in aged animals.

View Article and Find Full Text PDF

Rapid postharvest senescence and quality deterioration severely limit logistics of cowpeas. Melatonin (MEL) is a pivotal bioactive molecule that can modulate multiple physiological attributes in plants. In this study, physiological, transcriptomic and metabolomic analyses were conducted to explore the effects of exogenous MEL on cowpea senescence and its underlying mechanisms.

View Article and Find Full Text PDF

High susceptibility of mangoes to low temperature leads to ripening failure that restricts the marketability of products. This study investigated the effect of methyl jasmonate (MeJA) on ripening disorder and mechanism involved in mangoes during refrigeration. Results showed that 50 μM MeJA ameliorated ripening disorder, as indicated by accelerated advancement of ripening-related parameters.

View Article and Find Full Text PDF

Selenium-enriched (SL) is a kind of edible fungi rich in organic selenium and nutrients. with high monacolin K (MK) production and were selected as the fermentation strains. A single-factor experiment and response surface methodology were conducted to optimize the production conditions for MK with higher contents from selenium-enriched fermentation (SLF).

View Article and Find Full Text PDF
Article Synopsis
  • Disease risk prediction can be enhanced by analyzing genomic sequences and transcriptional profiles, though identifying harmful non-coding DNA variations remains challenging for common diseases.
  • This study explores the interactions between P53 and cMYC proteins in regulation of target genes, revealing co-occupancy patterns in DNA regions that are conserved through evolution.
  • Results indicate that doxorubicin treatment leads to changes in protein levels and gene expression, suggesting a method to connect non-coding variations to gene regulation and disease risk screening.
View Article and Find Full Text PDF

Background: Early and appropriate antibiotic treatment improves the clinical outcome of patients with sepsis. There is an urgent need for rapid identification (ID) and antimicrobial susceptibility testing (AST) of bacteria that cause bloodstream infection (BSI). Rapid ID and AST can be achieved by short-term incubation on solid medium of positive blood cultures using MALDI-TOF mass spectrometry (MS) and the BD M50 system.

View Article and Find Full Text PDF
Article Synopsis
  • Monascus ruber M7, a type of fermentation fungus, shows significant potential for increasing the production of orange and red pigments used in red yeast rice through selenium bio-fortification, with an increase of 38.52% and 36.57%, respectively, under specific conditions.
  • The addition of selenium reduced the levels of harmful mycotoxin citrinin in the yeast from 244.47 μg/g to 175.01 μg/g, which is a positive outcome for food safety.
  • Transcriptome analysis showed that certain genes responsible for pigment production were upregulated while those linked to mycotoxin production were downregulated, indicating a shift in metabolic processes facilitated by selenium.
View Article and Find Full Text PDF

Recent findings indicate that air pollution contributes to the onset and advancement of chronic obstructive pulmonary disease (COPD). Nevertheless, there is insufficient research indicating that air pollution is linked to COPD in the region of inland northwest China. Daily hospital admission records for COPD, air pollutant levels, and meteorological factor information were collected in Jiuquan for this study between 1 January 2018 and 31 December 2019.

View Article and Find Full Text PDF

The regenerative treatment of infectious vertical bone defects remains difficult and challenging today. Current clinical treatments are limited in their ability to control bacteria and infection, which is unfavorable for new bone formation and calls for a new type of material with excellent osteogenic and antibacterial properties. Here a multifunctional scaffold is synthesized that mimics natural bone nanostructures by incorporating silver nanowires into a hierarchical, intrafibrillar mineralized collagen matrix (IMC/AgNWs), to achieve the therapeutic goals of inhibiting bacterial activity and promoting infectious alveolar bone augmentation in rats and beagle dogs.

View Article and Find Full Text PDF

Background: This case involves a 28-year-old pregnant woman (39w+2) who was admitted to obstetrics due to abdominal tightness and bacteremia with Gardnerella vaginalis which developed after caesarean section and vaginal myomectomy.

Methods: A blood culture was performed, and the bacteria were identified through mass spectrometry.

Results: Mass spectrometry data indicated that the infection bacteria were Gardnerella vaginalis.

View Article and Find Full Text PDF

Pyroptosis, an inflammatory caspase-dependent programmed cell death, plays a vital role in maintaining tissue homeostasis and activating inflammatory responses. Orthodontic tooth movement (OTM) is an aseptic force-induced inflammatory bone remodeling process mediated by the activation of periodontal ligament (PDL) progenitor cells. However, whether and how force induces PDL progenitor cell pyroptosis, thereby influencing OTM and alveolar bone remodeling remains unknown.

View Article and Find Full Text PDF

Citrinin (CIT), a secondary metabolite produced by the filamentous fungi species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably restricting the utilization of -derived products. CIT synthesis is mediated through the gene and modified by multiple genetic factors. Here, the regulatory effects of two transcripts, α, and β, generated via pre-mRNA alternative splicing (AS), were investigated using hairpin RNA (ihpRNA) interference, and their impact on CIT biosynthesis and the underlying mechanisms were assessed through chemical biology and transcriptome analyses.

View Article and Find Full Text PDF

Bone infection poses a major clinical challenge that can hinder patient recovery and exacerbate postoperative complications. This study has developed a bioactive composite scaffold through the co-assembly and intrafibrillar mineralization of collagen fibrils and zinc oxide (ZnO) nanowires (IMC/ZnO). The IMC/ZnO exhibits bone-like hierarchical structures and enhances capabilities for osteogenesis, antibacterial activity, and bacteria-infected bone healing.

View Article and Find Full Text PDF

Infected bone defects are a major challenge in orthopedic treatment. Native bone tissue possesses an endogenous electroactive interface that induces stem cell differentiation and inhibits bacterial adhesion and activity. However, traditional bone substitutes have difficulty in reconstructing the electrical environment of bone.

View Article and Find Full Text PDF

Maintaining the stemness of bone marrow mesenchymal stem cells (BMMSCs) is crucial for bone homeostasis and regeneration. However, expansion and bone diseases impair BMMSC stemness, limiting its functionality in bone tissue engineering. Using a deep learning-based efficacy prediction system and bone tissue sequencing, we identify a natural small-molecule compound, dihydroartemisinin (DHA), that maintains BMMSC stemness and enhances bone regeneration.

View Article and Find Full Text PDF

Adult tendon stem/progenitor cells (TSPCs) are essential for tendon maintenance, regeneration, and repair, yet they become susceptible to senescence with age, impairing the self-healing capacity of tendons. In this study, we employ a recently developed deep-learning-based efficacy prediction system to screen potential stemness-promoting and senescence-inhibiting drugs from natural products using the transcriptional signatures of stemness. The top-ranked candidate, prim-O-glucosylcimifugin (POG), a saposhnikovia root extract, could ameliorate TPSC senescent phenotypes caused by long-term passage and natural aging in rats and humans, as well as restore the self-renewal and proliferative capacities and tenogenic potential of aged TSPCs.

View Article and Find Full Text PDF

Bast fiber textiles have become increasingly popular as a sustainable alternative in recent years. Although the carbon emissions of bast fiber textiles have been studied using life cycle assessment method, there is a lack of comprehensive literature analyzing and summarizing the results. This study reviews the current state of research on the carbon emissions of bast fiber textiles.

View Article and Find Full Text PDF
Article Synopsis
  • * The study focused on the co-occupancy and competitive interactions between the transcription factors P53 and cMYC in regulating gene expression, identifying that approximately 344-366 genomic regions were co-occupied by both factors in human and mouse embryonic stem cells.
  • * Treatment with doxorubicin altered protein levels of P53 and cMYC in certain cell types and resulted in significant changes in the expression of nearby genes, indicating that variations in non-coding DNA influence the binding affinity of these transcription factors, which in turn affects gene regulation
View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessioniemv297ucptmd1armhosog9ib7op3cp7): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once