Power-to-vitamins: producing folate (vitamin B) from renewable electric power and CO with a microbial protein system.

We recently proposed a two-stage Power-to-Protein technology to produce microbial protein from renewable electric power and CO. Two stages were operated in series: Clostridium ljungdahlii in Stage A to reduce CO with H into acetate, and Saccharomyces cerevisiae in Stage B to utilize O and produce microbial protein from acetate. Renewable energy can be used to power water electrolysis to produce H and O.

Larval description of Theloderma albopunctatum (Liu & Hu, 1962) (Anura: Rhacophoridae) from Northern Vietnam, with comparison between the North-Vietnamese and North-eastern Thai clades.

The mossy frogs of the genus Theloderma Tschudi comprise 28 described taxa (Sivongxay et al. 2016; Frost 2022), which are distributed from north-eastern India and Myanmar to southern China, across the peninsula of Indochina and Malaysia, to Indonesia (Poyarkov et al. 2015; Frost 2022).

Recombinant expression and characterization of novel P450s from Actinosynnema mirum.

Cytochrome P450 monooxygenases (P450s) are the major contributor in the metabolism of xenobiotics, including therapeutic agents. Thus, P450s find broad application in the pharmaceutical industry to synthesize metabolites of new active pharmaceutical ingredients in order to evaluate toxicity and pharmacokinetics. As an alternative to human hepatic P450s, microbial P450s offer several advantages, such as an easier and more efficient heterologous expression as well as higher stability under process conditions.

Investigation of Vitamin D and Vitamin D Hydroxylation by Kutzneria albida.

The active vitamin D metabolites 25-OH-D and 1α,25-(OH) -D play an essential role in controlling several cellular processes in the human body and are potentially effective in the treatment of several diseases, such as autoimmune diseases, cardiovascular diseases and cancer. The microbial synthesis of vitamin D (VD ) and vitamin D (VD ) metabolites has emerged as a suitable alternative to established complex chemical syntheses. In this study, a novel strain, Kutzneria albida, with the ability to form 25-OH-D and 25-OH-D was identified.

Recent advances in heme biocatalysis engineering.

Heme enzymes have the potential to be widely used as biocatalysts due to their capability to perform a vast variety of oxidation reactions. In spite of their versatility, the application of heme enzymes was long time-limited for the industry due to their low activity and stability in large scale processes. The identification of novel natural biocatalysts and recent advances in protein engineering have led to new reactions with a high application potential.

Promoters from the itaconate cluster of are induced by nitrogen depletion.

Background: is known for its natural potential to produce a broad range of valuable chemicals, such as itaconate, from both industrial carbon waste streams and renewable biomass. Production of itaconate, and many other secondary metabolites, is induced by nitrogen limitation in . The clustered genes responsible for itaconate production have recently been identified, enabling the development of new expression tools that are compatible with biotechnological processes.

Enzyme-Based Electrobiotechnological Synthesis.

Oxidoreductases are enzymes with a high potential for organic synthesis, as their selectivity often exceeds comparable chemical syntheses. The biochemical cofactors of these enzymes need regeneration during synthesis. Several regeneration methods are available but the electrochemical approach offers an efficient and quasi mass-free method for providing the required redox equivalents.