Development and Application of a High Throughput Protein Unfolding Kinetic Assay.

The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plate reader that is applicable to the studies of the majority of 2-state folding proteins. We validate the assay by measuring kinetic unfolding data for the SH3 (Src Homology 3) domain from Actin Binding Protein 1 (AbpSH3) and its stabilized mutants.

Humanizing the homeless: does contact erode stereotypes?

This paper employs a field experiment to assess whether interpersonal contact changes domiciled individuals' attitudes of the homeless. Volunteers for Project Homeless Connect-a one-day event that provides social services to the homeless-were asked to complete a pre- and post-survey. The results provide mixed support for the contact hypothesis.