Adoptive transfer of natural killer cells promotes the anti-tumor efficacy of T cells.

The density of NK cells in tumors correlates positively with prognosis in many types of cancers. The average number of infiltrating NK cells is, however, quite modest (approximately 30 NK cells/sq.mm), even in tumors deemed to have a "high" density of infiltrating NK cells.

Secretory TRAIL-Armed Natural Killer Cell-Based Therapy: In Vitro and In Vivo Colorectal Peritoneal Carcinomatosis Xenograft.

Since its discovery in 1995, TNF-related apoptosis-inducing ligand (TRAIL) has sparked growing interest among oncologists due to its remarkable ability to induce apoptosis in malignant human cells, but not in most normal cells. However, one major drawback is its fast clearance rate in vivo Thus, the development of an alternative means of delivery may increase the effectiveness of TRAIL-based therapy. In this study, we developed a secretory TRAIL-armed natural killer (NK) cell-based therapy and assessed its cytotoxic effects on colorectal cancer cells and its tumoricidal efficacy on colorectal peritoneal carcinomatosis xenograft.

Meta-Analysis of Soybean-based Biodiesel.

Biofuel policy changes in the United States have renewed interest in soybean [ (L.) Merr.] biodiesel.

The impact of calcium assay change on a local adjusted calcium equation.

Background: Deriving and validating local adjusted calcium equations is important for ensuring appropriate calcium status classification. We investigated the impact on our local adjusted calcium equation of a change in calcium method by the manufacturer from cresolphthalein complexone to NM-BAPTA.

Methods: Calcium and albumin results from general practice requests were extracted from the Laboratory Information Management system for a three-month period.

Measurement of teicoplanin by liquid chromatography-tandem mass spectrometry: development of a novel method.

Background: Teicoplanin is an antibiotic used for the treatment of endocarditis, osteomyelitis, septic arthritis and methicillin-resistant Staphylococcus aureus. Teicoplanin is emerging as a suitable alternative antibiotic to vancomycin, where their trough serum levels are monitored by immunoassay routinely. This is the first report detailing the development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring teicoplanin in patients' serum.

A novel molecular mechanism to explain biotin-unresponsive holocarboxylase synthetase deficiency.

Biotin (vitamins H and B7) is an important micronutrient as defects in its availability, metabolism or adsorption can cause serious illnesses, especially in the young. A key molecule in the biotin cycle is holocarboxylase synthetase (HLCS), which attaches biotin onto the biotin-dependent enzymes. Patients with congenital HLCS deficiency are prescribed oral biotin supplements that, in most cases, reverse the clinical symptoms.

IL-4 suppresses very late antigen-4 expression which is required for therapeutic Th1 T-cell trafficking into tumors.

Murine CD4 T cells cultured under type 1 polarizing conditions selectively express significantly higher levels of the very late antigen (VLA)-4 and VLA-6 integrins when compared with T cells cultured under type 2 or nonpolarizing (type 0) conditions. This difference appears due to the action of interleukin (IL)-4, as loss of VLA-4/-6 expression on Th cells was prevented by inclusion of neutralizing anti-IL-4 mAb during the initial culture period. We also observed that CD4 T cells deficient in Stat6, a critical component of the IL-4R signaling cascade, retained high levels of VLA-4 and VLA-6 expression, regardless of IL-4 status in the culture conditions.

Biotin protein ligase from Candida albicans: expression, purification and development of a novel assay.

Biotin protein ligase (BPL) is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin. In yeast, disruption of BPL affects important metabolic pathways such as fatty acid biosynthesis and gluconeogenesis. This makes BPL an attractive drug target for new antifungal agents.

Microbial biotin protein ligases aid in understanding holocarboxylase synthetase deficiency.

The attachment of biotin onto the biotin-dependent enzymes is catalysed by biotin protein ligase (BPL), also known as holocarboxylase synthase HCS in mammals. Mammals contain five biotin-enzymes that participate in a number of important metabolic pathways such as fatty acid biogenesis, gluconeogenesis and amino acid catabolism. All mammalian biotin-enzymes are post-translationally biotinylated, and therefore activated, through the action of a single HCS.

Reduced half-life of holocarboxylase synthetase from patients with severe multiple carboxylase deficiency.

Multiple carboxylase deficiency is a clinical condition caused by defects in the enzymes involved in biotin metabolism, holocarboxylase synthetase (HLCS) or biotinidase. HLCS deficiency is a potentially fatal condition if left untreated, although the majority of patients respond to oral supplementation of 10-20 mg/day of biotin. Patients who display incomplete responsiveness to this therapy have a poor long-term prognosis.

The association of circulating ferritin with serum concentrations of fibroblast growth factor-23 measured by three commercial assays.

Background: The measurement of the serum concentration of fibroblast growth factor-23 (FGF-23) is beginning to be used as a diagnostic tool in renal phosphate wasting disorders. Having observed an increased serum FGF-23 in three subjects with low circulating ferritin concentrations we investigated the association between low ferritin and raised serum FGF-23.

Methods: We measured FGF-23 in 150 random anonymized serum samples with ferritin concentrations between <5 and 50 microg/L using three commercially available enzyme-linked immunosorbent assay (ELISA) kits.

A large-scale study of handedness and pregnancy/birth risk events: implications for genetic theories of handedness.

Whether left-handedness is due to genetic factors or to pregnancy and birth stress events is an important question for models that attempt to explain the origins and distribution of human handedness. Major genetic theories of handedness, such as those of Annett (1985) and McManus (1981), allow for a nondetermination of some left- or right-handedness by "chance", but they hold that "pathological left-handedness", specifically, is of minimal influence. On the other hand, theorists such as Bakan (1971) and Coren (1995) take the view that right-handedness is a universal human characteristic, presumably due to a polygenic influence, and that left-handedness is essentially always the product of pregnancy and birth risk factors.

Strength of religious faith and psychological adjustment in intercollegiate athletes.

In light of recent research examining the distress buffering properties of religion in intercollegiate athletes' lives, the present study investigated associations among religious faith and depressive symptoms, trait anxiety, and loneliness. Using self-report questionnaires, religious faith was not correlated with depressive symptoms, trait anxiety, and loneliness in 57 intercollegiate athletes.