Publications by authors named "Lisa J Calton"
Article Synopsis
- The UK's Department of Health initiated the Moonshot programme in 2020 to enhance SARS-CoV-2 testing, exploring various diagnostic technologies, including mass spectrometry.
- A targeted protein assay was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) to detect specific peptides from the virus in nasopharyngeal swabs, and its effectiveness was compared with the standard RT-qPCR method.
- The LC-MS/MS method showed high sensitivity (92.4%) and specificity (97.4%) for detecting SARS-CoV-2, validating its potential for faster clinical application and setting a model for future diagnostic innovations.
The introduction of tandem mass spectrometry (MS/MS) to clinical laboratories and the advent of expanded newborn screening (NBS) were crucial changes to public health programs worldwide. Speed, robustness, accuracy, selectivity, and specificity of analysis are all requirements of expanded NBS and are needed to minimize false positive results risks, to possibly eliminate false negatives, and to improve the positive predictive value of NBS. In this study, we firstly evaluated the analytical performances of the RenataDX Screening System, a fully integrated flow-injection MS/MS (FIA-MS/MS) IVD system for high-throughput dried blood spot (DBS) analysis in a routine NBS laboratory.
View Article and Find Full Text PDFBackground: LC-MS is increasingly used for therapeutic drug monitoring of tacrolimus. A recent summary from an international proficiency-testing scheme demonstrated that the mass spectrometry respondents were the largest method group. However, these methods lack standardization, which may explain the relatively poor interlaboratory agreement for such methods.
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