Publications by authors named "Lisa Fourniol"
Article Synopsis
- Tick-borne encephalitis (TBE) outbreaks in Europe have been linked to the consumption of raw milk products, highlighting a need for better detection methods of the virus in these foods.
- Researchers developed a sensitive cell culture-based method for detecting infectious TBEV in artificially contaminated raw goat milk and cheese, achieving varying levels of viral detection.
- The study showed that this method successfully identified infectious TBEV in both milk and cheese samples, supporting its potential use in future foodborne outbreak investigations.
Human norovirus and hepatitis viruses (hepatitis A (HAV) and hepatitis E (HEV)) are leading causes of foodborne disease worldwide. Among the various food products, different types of dairy products can be implicated in viral foodborne outbreaks and contamination can occur at different stages, such as preparation, contact with contaminated equipment or via other foods. The aim of this study was to characterise a proteinase K method adapted from the ISO 15216 method for the detection of HAV, HEV and norovirus in artificially contaminated dairy products, based on the recent international standard of ISO 16140-4.
View Article and Find Full Text PDFThe transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4.
View Article and Find Full Text PDFMosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito.
View Article and Find Full Text PDFArticle Synopsis
- - The study focused on creating synthetic peptide vaccines aimed at neuropeptides in salivary glands and hindguts of ticks to protect mice and sheep from tick infestations.
- - Vaccination generated a strong IgG antibody response against SIFamide (SIFa) and myoinhibitory peptide (MIP) neuropeptides, but did not show significant negative effects on nymphs in mice or clear conclusions in sheep due to low nymph numbers.
- - While overall tick feeding and growth were not notably affected, a negative link was found between antibody levels against SIFa and MIP and the success of larva molting, suggesting potential for using MAPs to target tick immunity, despite limited protection results
To identify potential vaccine candidates against and tick-borne pathogen transmission, we have previously sequenced the salivary gland transcriptomes of female ticks infected or not with The hypothesized potential of both IrSPI ( serine protease inhibitor) and IrLip1 ( lipocalin 1) as protective antigens decreasing tick feeding and/or the transmission of tick-borne pathogens was based on their presumed involvement in dampening the host immune response to tick feeding. Vaccine endpoints included tick larval and nymphal mortality, feeding, and molting in mice and sheep. Whether the antigens were administered individually or in combination, the vaccination of mice or sheep elicited a potent antigen-specific antibody response.
View Article and Find Full Text PDFArticle Synopsis
- A study established a sheep infection model for the tick-borne diseases transmitted by the European Norway variant 2 ovine strain (NV2Os), showing successful infection through tick cells.
- Both inoculated sheep exhibited clinical signs of infection such as fever, the presence of specific bacteria in blood tests, and antibody development.
- The research confirmed that ticks can effectively transmit the bacteria between sheep, demonstrating the potential for further studies on transmission and control strategies for this emerging disease in Europe.